Mice heterozygous for this semi-dominant spontaneous mutation of the calcium channel, voltage-dependent, P/Q type, alpha 1A subunit gene have a shaky gait, but do not exhibit dyskinesia or absence seizures. The Tottering-5J mutation causes a shift in voltage activation and inactivation to lower voltages. This strain may be useful for studies of epilepsy and voltage-dependent calcium channels.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Cacna1a | calcium channel, voltage-dependent, P/Q type, alpha 1A subunit |
Mice heterozygous for the semi-dominant Tottering 5 Jackson mutation exhibit a shaky gait with hind limbs splayed laterally from the body in contrast to the other tottering mutants . Also, unlike tottering mice, Tg-5J heterozygotes do not exhibit dyskinesia or absence seizures. Axonal swelling is observed in Purkinje cells from homozygotes as well as a decrease in branching. The Tg-5J mutation causes a shift in voltage activation and inactivation to lower voltages. This strain may be useful for studies of epilepsy and voltage-dependent calcium channels.
The C57BL/10J background appears to be embryonic lethal as no homozygotes are produced in heterozygote x heterozygote matings. On a mixed BALB/cByJ and C57BL/10J background some homozygotes are produced, but die before 6 weeks of age.
This spontaneous mutation arose in strain B10.M-H2f/nMob Fmn1ld-2J/J (Stock No. 002225) at The Jackson Laboratory in 1995. It was backcrossed a minimum of five generations to C57BL/10J in the laboratory of Dr. Wayne Frankel and Dr. Verity Letts and donated to the Repository in 2008.
Allele Name | tottering 5 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Cacna1a, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit |
Gene Synonym(s) | |
Strain of Origin | B10.M-H2f/nMob Fmn1ld-2J/J |
Chromosome | 8 |
Molecular Note | A G to A substitution at coding cDNA nucleotide 3899 (c.3899G>A) results in a change of arginine codon 1300 to glutamine (p.R1300Q). |
When maintaining a live colony, these mice are bred as heterozygotes. On the C57BL/10J background, homozygous mice die by before birth.
When using the B10.Cg-Cacna1aTg-5J/LetJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #008623 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Cacna1a<Tg-5J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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