Mice homozygous for this spontaneous mutation of the calcium channel, voltage-dependent, P/Q type, alpha 1A subunit gene have an ataxic gait, paroxysomal dyskinesia and absence seizures. Tottering 4J mice may be useful for studies of epilepsy and voltage-dependent calcium channels.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Cacna1a | calcium channel, voltage-dependent, P/Q type, alpha 1A subunit |
Homozygotes for the tottering 4 Jackson allele exhibit a phenotype similar to that of mice homozygous for the tottering allele. Mice have an ataxic gait, with high stepping, waggling movements and paroxysomal dyskinesia. EEG recordings reveal 5-7 Hz spike wave discharges accompanied by behavioral arrest lasting up to 1.8 sec and ocurring an average of four times per hour. This seizure phenotype is less severe than in tottering mice. Unique among the tottering alleles, tg-4J causes altered activation and inactivation kinetics of the voltage-dependent calcium channel. This strain may be useful for studies of epilepsy and voltage-dependent calcium channels.
This spontaneous mutation arose at The Jackson Laboratory in a colony of C3H/HeJBir in the laboratory of Dr. Edward Birkenmeier in 1996. It was backcrossed to C57BL/6J for a minimum of 10 generations in the laboratory of Dr. Wayne Frankel and Dr. Verity Letts. The strain was donated to the Repository in 2008.
Allele Name | tottering 4 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Cacna1a, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit |
Gene Synonym(s) | |
Strain of Origin | C3H/HeJBir |
Chromosome | 8 |
Molecular Note | A T to C substitution at coding cDNA nucleotide 1886 (c.1886T>C) results in a change of valine codon 629 to alanine (p.V629A). |
When maintaining a live colony, these mice are bred as homozygotes.
When using the B6.C3Bir-Cacna1atg-4J/LetJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #008622 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Cacna1a<tg-4J> |
Frozen Mouse Embryo | B6.C3Bir-Cacna1a<tg-4J>/LetJ | $2595.00 |
Frozen Mouse Embryo | B6.C3Bir-Cacna1a<tg-4J>/LetJ | $2595.00 |
Frozen Mouse Embryo | B6.C3Bir-Cacna1a<tg-4J>/LetJ | $3373.50 |
Frozen Mouse Embryo | B6.C3Bir-Cacna1a<tg-4J>/LetJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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