A portion of the X-linked mouse opsin 1 (medium-wave-sensitive) "green pigment" gene was replaced by a human opsin 1 (long-wave-sensitive) "red pigment" cDNA in these targeted mutant mice. Heterozyguous female mice are endowed with a unique ability to make chromatic discriminations at long wavelengths. Hemizygous males and homozygous females have greater sensitivity to long wavelength light than normal mice.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Inserted expressed sequence, Humanized sequence) | Opn1mw | opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan) |
A portion of the X-linked mouse opsin 1 (medium-wave-sensitive) "green pigment" gene was replaced by a human opsin 1 (long-wave-sensitive) "red pigment" cDNA in these targeted mutant mice. Heterozyguous female mice are endowed with a unique ability to make chromatic discriminations at long wavelengths. Hemizygous males and homozygous females have greater sensitivity to long wavelength light than normal mice. Heterozygotes, hemizygotes, and homozygotes are normal in size, viability, and fertility. Expression is observed in retinal cone photoreceptors as determined by immunostaining. The knocked-in coding sequences are expressed normally.
The 3' portion of exon 2, and exons 3-5 of the x-linked mouse opsin 1 (medium-wave-sensitive) "green pigment" gene were replaced by a human opsin 1 (long-wave-sensitive) "red pigment" cDNA. The targeted mutation was introduced using a 129-derived embryonic stem (ES) cell line and backcrossed 6 times to C57BL/6 (see SNP note below) by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | OPN1LW, opsin 1, long wave sensitive, human |
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Site of Expression |
Allele Name | targeted mutation 1, Jeremy Nathans |
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Allele Type | Targeted (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Opn1mwR; Opn1mwred; R |
Gene Symbol and Name | Opn1mw, opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan) |
Gene Synonym(s) | |
Expressed Gene | OPN1LW, opsin 1, long wave sensitive, human |
Strain of Origin | 129 |
Chromosome | X |
Molecular Note | Human cDNA encoding OPN1LW was inserted at the endogenous mouse Opn1mw locus, replacing a 3' portion of exon 2 and all of exons 3, 4, and 5. The allele putatively produces a hybrid protein consisting of 92 N-terminal residues encoded by mouse exon 1 and the 5' portion of exon 2, and 266 C-terminal residues encoded by the human cDNA. Nearly the entire chromophore binding pocket, as well as the residues that account for the difference in absorbance spectra between human and mouse, is derived from the human sequence. Immunostaining indicated expression of the hybrid protein and electroretinogram indicated that the spectral sensitivity of cones containing the targeted allele was similar to that of human red cones. |
The mutation in this strain is X-linked. When maintained as a live colony, heterozygous (female), hemizygous (male), or homozygous (female) mice may be bred.
When using the B6.129-Opn1mwtm1(OPN1LW)Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008619 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous females and Wild-type males for Opn1mw<tm1(OPN1LW)Nat> |
Frozen Mouse Embryo | B6.129-Opn1mw<tm1(OPN1LW)Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Opn1mw<tm1(OPN1LW)Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Opn1mw<tm1(OPN1LW)Nat>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Opn1mw<tm1(OPN1LW)Nat>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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