B6(A)-Tg(OPN1LW-DT)1Mame/J

Stock number: 008617
Research areas: Neurobiology Research, Sensorineural Research

Description

Cone photoreceptors in these mice express a modified (DT176) diptheria toxin A chain (DTA) driven by the human red cone specific opsin promoter. This results in the ablation of the cone cells. This strain may be useful in studies of vision and signal processing within the central nervous system.

Detailed description

Cone photoreceptors in these mice express a modified (DT176) diptheria toxin A chain (DTA) driven by the human red cone specific opsin promoter. This results in the ablation of the cone cells. These mice show no physical, behavioral, reproductive, or growth abnormalities, and the histologic appearance of their retinae remains unchanged over at least 8 months. This strain may be useful in studies of vision and signal processing within the central nervous system.

Development

The transgenic construct was made by joining a 6.5 kb human red pigment gene (opsin 1 (cone pigments), long-wave-sensitive) 5' region with the coding cassette of a modified (DT176) DTA (diphtheria toxin, A chain). The vector was introduced to F2 embryos from a cross of C57BL/6 x BAF1 animals and maintained on that mixed background by the donating investigator.

Allele

Symbol: Tg(OPN1LW-DT)1Mame
Name: transgene insertion 1, Markus Meister
MGI accession ID: MGI:3767977
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: cl; coneless; h.red DT-A; Red-DT(A)
Marker symbol: Tg(OPN1LW-DT)1Mame
Marker name: transgene insertion 1, Markus Meister
Marker synonyms: cl; coneless; h.red DT-A; Red-DT(A)
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: Dta,Diphtheria toxin A chain,
Molecular note: The human red cone specific opsin (OPN1LW) promoter region was used to drive expression of diphtheria toxin A in cone cells.