Cone photoreceptors in these mice express a modified (DT176) diptheria toxin A chain (DTA) driven by the human red cone specific opsin promoter. This results in the ablation of the cone cells. This strain may be useful in studies of vision and signal processing within the central nervous system.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Cone photoreceptors in these mice express a modified (DT176) diptheria toxin A chain (DTA) driven by the human red cone specific opsin promoter. This results in the ablation of the cone cells. These mice show no physical, behavioral, reproductive, or growth abnormalities, and the histologic appearance of their retinae remains unchanged over at least 8 months. This strain may be useful in studies of vision and signal processing within the central nervous system.
The transgenic construct was made by joining a 6.5 kb human red pigment gene (opsin 1 (cone pigments), long-wave-sensitive) 5' region with the coding cassette of a modified (DT176) DTA (diphtheria toxin, A chain). The vector was introduced to F2 embryos from a cross of C57BL/6 x BAF1 animals and maintained on that mixed background by the donating investigator.
Expressed Gene | Dta, Diphtheria toxin A chain, |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, Markus Meister |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | cl; coneless; h.red DT-A; Red-DT(A) |
Gene Symbol and Name | Tg(OPN1LW-DT)1Mame, transgene insertion 1, Markus Meister |
Gene Synonym(s) | |
Promoter | OPN1LW, opsin 1, long wave sensitive, human |
Expressed Gene | Dta, Diphtheria toxin A chain, |
Strain of Origin | Not Applicable |
Chromosome | UN |
Molecular Note | The human red cone specific opsin (OPN1LW) promoter region was used to drive expression of diphtheria toxin A in cone cells. |
Mutations Made By | Jeremy Nathans, Johns Hopkins University |
When maintained as a live colony, hemizygotes may be bred.
When using the B6(A)-Tg(OPN1LW-DT)1Mame/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008617 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(OPN1LW-DT)1Mame |
Frozen Mouse Embryo | B6(A)-Tg(OPN1LW-DT)1Mame/J | $2595.00 |
Frozen Mouse Embryo | B6(A)-Tg(OPN1LW-DT)1Mame/J | $2595.00 |
Frozen Mouse Embryo | B6(A)-Tg(OPN1LW-DT)1Mame/J | $3373.50 |
Frozen Mouse Embryo | B6(A)-Tg(OPN1LW-DT)1Mame/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.