Overview

Also Known As: TH-Cre 1 , TH-1 Cre

TH-Cre transgenic mice have the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to catecholaminergic cells, and may be useful for generating conditional mutations in these cells for studying dopaminergic cell function, Parkinson's disease, and other neurodegenerative disorders.

In Goodwin et al. 2017 bioRxiv, it was discovered that the transgene integrated into chromosome 9 causing a 624 kbp deletion in the 7630403G23Rik locus.

Donating Investigator

Ted M Dawson, Johns Hopkins Univ School of Medicine

Genetic overview

Genetic Background	Generation
	N6+F23 (2018-12-21 00:00:00)

7630403G23Rik^{Tg(Th-cre)1Tmd}

Allele Type
Transgenic (Recombinase-expressing)

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Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$255.00 Domestic price for female

333.51 Domestic price for breeder pair

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Details

Detailed Description

These transgenic mice express Cre recombinase under the control of the rat tyrosine hydroxylase (TH) promoter. The transgene integrated into chromosome 9 causing a 624 kbp deletion in 7630403G23Rik locus. Mice hemizygous for the TH-Cre transgene are viable and fertile, with the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to catecholaminergic cells. Using crosses to reporter strains, cre activity is confirmed in catecholaminergic cells and is present in many of the projection areas of these neuronal populations. A mosaic of cre activity is noted in TH-positive neurons. Several other areas that are not typically thought to have active TH expression, including the lateral septal nucleus, accessory olfactory bulb, suparafascicular thalamus, and pretectal area, also exhibit Cre recombinase activity (possibly as a result of TH promoter activity in precursor cell populations or ectopic expression from the exogenous TH promoter). Some TH-negative cells closely clustered around and within TH-positive nuclei demonstrate cre activity. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These TH-Cre transgenic mice are a tool for generating conditional mutations in catecholaminergic cells and may be useful for studying dopaminergic cell function, Parkinson's disease, and other neurodegenerative disorders.

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (7630403G23Rik^{Tg(Th-cre)1Tmd}). This same information would also be found searching the MGI Recombinase Activity database.

Development

The TH-Cre transgene was designed with a 9.0 kb fragment of the rat tyrosine hydroxylase (TH) promoter, synthetic 5' UTR intron, cre cDNA, and SV40 late region polyadenylation site. The transgene was microinjected into the pronuclei of fertilized B6/SJLF2 oocytes. Founder mice were bred to C57BL/6NCrl to confirm germline transmission and establish founder line 1 (TH-Cre 1). The transgene integrated into chromosome 9 causing a 624 kbp deletion in 7630403G23Rik locus. Founder line 1 has a copy number of greater than 20. Founder line 1 has a copy number of 10-15 The donating investigator reported that the TH-Cre 1 transgenic colony was subsequently maintained by backcrossing to C57BL/6 (Charles River) mice for greater than five generations prior to arrival at The Jackson Laboratory Repository in 2009.
Upon arrival, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony. Thereafter, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) mice from the colony. In 2014, SNP analysis suggests these mice are predominantly on a C57BL/6N genetic background (see SNP note below).

In 2010 and 2011, a 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony (generation N6+F2, N6+F4 and N6+F6) at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating (each mouse having at least 1 marker segregating). These data suggest the mice sent to The Jackson Laboratory Repository in 2009 were on a C57BL/6J or mixed C57BL/6J;C57BL/6N genetic background. In 2014, a cohort of mice from generation N6+F14 had 4 of the 5 markers that determine C57BL/6J from C57BL/6N fixed to C57BL/6N allele-type, with a single marker on chromosome 13 (~41.0 Mbp) fixed to C57BL/6J allele-type.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	dopaminergic neurons

Control Suggestions

Noncarrier

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Savitt JM; Jang SS; Mu W; Dawson VL; Dawson TM. 2005. Bcl-x is required for proper development of the mouse substantia nigra. J Neurosci 25(29):6721-8PubMed: 16033881 MGI: J:99848

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Genetics

7630403G23Rik^{Tg(Th-cre)1Tmd}

Allele Symbol: 7630403G23Rik^{Tg(Th-cre)1Tmd}

Allele Name	transgene insertion 1, Ted M Dawson
Allele Type	Transgenic (Recombinase-expressing)
Allele Synonym(s)	transgene insertion 1, Ted M Dawson; 7630403G23Rik^{Tg(Th-cre)1Tmd}
Gene Symbol and Name	7630403G23Rik, RIKEN cDNA 7630403G23 gene
Gene Synonym(s)
Promoter	Th, tyrosine hydroxylase, rat
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	dopaminergic neurons
Strain of Origin	(C57BL/6 x SJL)F2
Chromosome	9
Molecular Note	Cre recombinase is expressed under the control of the rat tyrosine hydroxylase promoter. Cre recombinase activity is detected in catecholaminergic cells. This is a representative record representing TH-cre1 - TH-cre5 all of which have identical activity. Line 1 inserted into the gene at 33514690-34139124 (Build GRCm38/mm10) resulting in a 624,434 bp deletion. Founder line 1 has a copy number of greater than 20.
Mutations Made By	Ted Dawson, Johns Hopkins Univ School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue
- Parkinson's Disease
  - strains expressing cre
Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers

Mammalian Phenotype Terms by Genotype

References

Savitt JM; Jang SS; Mu W; Dawson VL; Dawson TM. 2005. Bcl-x is required for proper development of the mouse substantia nigra. J Neurosci 25(29):6721-8PubMed: 16033881 MGI: J:99848

Additional References

Daher JP; Ying M; Banerjee R; McDonald RS; Dumas Hahn M; Yang L; Beal MF; Thomas B; Dawson VL; Dawson TM; Moore DJ. 2009. Conditional transgenic mice expressing C-terminally truncated human alpha-synuclein (alphaSyn119) exhibit reduced striatal dopamine without loss of nigrostriatal pathway dopaminergic neurons. Mol Neurodegener 4(1):34PubMed: 19630976 MGI: J:150777

Additional - 7630403G23Rik^{Tg(Th-cre)1Tmd} related

Technical Support

Contact Technical Support

Genotyping Protocols
- MELT: Tg(Th-cre)1Tmd
- Standard PCR: Generic Cre
- Probe: 7630403G23Rik^{Tg(Th-cre)1Tmd}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6NJ inbred mice (Stock No. 005304). The donating investigator reports that homozygous mice are viable.
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
Citation
When using the TH-Cre 1 , TH-1 Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008601 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

FGB29 (Standard)

Pricing & Availability

Available

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Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or Non carrier for Tg(Th-cre)1Tmd

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: TH-Cre 1 , TH-1 Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

7630403G23RikTg(Th-cre)1Tmd

Allele Symbol: 7630403G23RikTg(Th-cre)1Tmd

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - 7630403G23RikTg(Th-cre)1Tmd related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

7630403G23Rik^{Tg(Th-cre)1Tmd}

Allele Symbol: 7630403G23Rik^{Tg(Th-cre)1Tmd}

Additional - 7630403G23Rik^{Tg(Th-cre)1Tmd} related