JAX
Mouse Strain Datasheet - 008599
B6.Cg-Del(9Cyp1a2-Cyp1a1)1Dwn Ahrd Tg(CYP1A1,CYP1A2)1Dwn/DwnJ
Also Known As: humanized hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahr<d> mutant mice
These "humanized" hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mice carry the human CYP1A1 and CYP1A2 genes in the absence of functional mouse Cyp1a1 and Cyp1a2 orthologs, and also mimic the human poor-affinity aryl hydrocarbon receptor (AHR) by carrying the poor-affinity Ahrd allele derived from DBA/2J mice. These may be useful in drug or carcinogen metabolism research; specifically as a model for human risk assessment studies involving drug or environmental toxicants that may be substrates for the aryl hydrocarbon receptor (AHR) or cytochrome P450 family 1 members.
Donating Investigator
Daniel W Nebert, University of Cincinnati Medical Center
Genetic overview
| Genetic Background | Generation |
|---|---|
|
|
Ahrd
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Not Applicable | Ahr | aryl-hydrocarbon receptor |
Del(9Cyp1a2-Cyp1a1)1Dwn
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Null/Knockout) | Del(9Cyp1a2-Cyp1a1)1Dwn | deletion, Chr 9, Daniel W Nebert 1 |
Tg(CYP1A1,CYP1A2)1Dwn
| Allele Type |
|---|
| Transgenic (Humanized sequence, Inserted expressed sequence) |
Research Applications
- Cancer Research
- Cell Biology Research
- Research Tools
- Metabolism Research
Detailed Description
These "humanized" hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mice carry the human CYP1A1 and CYP1A2 genes in the absence of functional mouse Cyp1a1 and Cyp1a2 orthologs, and also mimic the human poor-affinity aryl hydrocarbon receptor (AHR) by carrying the poor-affinity Ahrd allele derived from DBA/2J mice (rather than the high-affinity Ahrb1 allele normally present on a C57BL/6J genetic background); all on a C57BL/6J (reported >99.8%) genetic background.
Mice homozygous for the Cyp1a2/Cyp1a1 targeted allele [Cyp1a1/1a2(-/-)], homozygous for the Ahrd allele, and carrying the hCYP1A1_1A2 transgene are viable and fertile with normal lifespan. As the Cyp1a2/Cyp1a1(-) targeted allele lacks the coding regions of both Cyp1a1 and Cyp1a2 genes, no mouse CYP1A1 or CYP1A2 mRNA expression is observed in liver, lung or kidney. Transgene expression of the orthologous human genes is observed in the same tissues. Because expression of the hCYP1A1_1A2 transgene is controlled by the mouse AHR, the low-affinity Ahrd allele results in diminished CYP1A responses following exposure to AHR inducers (such as dioxin) when compared to hCYP1A1_1A2_Cyp1a1/1a2(-/-) mice harboring a high-affinity Ahr allele. These humanized hCYP1A1_1A2_Cyp1a2/Cyp1a1(-/-)_Ahrd mice may be useful in drug or carcinogen metabolism research; specifically as a model for human risk assessment studies involving drug or environmental toxicants that may be substrates for the aryl hydrocarbon receptor (AHR) or cytochrome P450 family 1 members.
Development
These "humanized" hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mutant mice were generated in the laboratory of Dr. Daniel W. Nebert (University of Cincinnati Medical Center). These mice were generated by breeding hCYP1A1_1A2_Cyp1a1/1a2(-/-) mice (see Stock No. 007580, but reportedly maintained on a >99.8% C57BL/6J genetic background) to B6.D2-Ahrd mice (Stock No. 002921). The resulting mice were subsequently bred with B6.D2-Ahrd mice for at least 2 generations to fix the Ahrd allele to homozygosity. Mice homozygous for the Cyp1a1/1a2 mutation, homozygous for the Ahrd allele, and carrying the hCYP1A1_1A2 transgene were sent to The Jackson Laboratory. Upon arrival, mice were bred with B6.D2-Ahrd mice (Stock No. 002921) for at least one generation to establish this colony.
Upon rederivation of these mice at The Jackson Laboratory, we will perform a genetic background analysis using a single nucleotide polymorphism (SNP) analysis. The results of this analysis have not yet been completed (December 2008).
Expression Data
| Expressed Gene | CYP1A1, cytochrome P450 family 1 subfamily A member 1, human |
|---|---|
| Expressed Gene | CYP1A2, cytochrome P450 family 1 subfamily A member 2, human |
| Site of Expression |
Control Suggestions
Selected References
- Shi Z; Chen Y; Dong H; Amos-Kroohs RM; Nebert DW. 2008. Generation of a 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mouse line harboring the poor-affinity aryl hydrocarbon receptor. Biochem Biophys Res Commun 376(4):775-80. PubMed: 18814841MGI: J:141523
Ahrd
Allele Symbol: Ahrd
| Allele Name | d variant |
|---|---|
| Allele Type | Not Applicable |
| Allele Synonym(s) | Ahd; Ahk; AhRd; Ahhn; ah; in |
| Gene Symbol and Name | Ahr, aryl-hydrocarbon receptor |
| Gene Synonym(s) | Ah; Ahh; Ahre; In; aromatic hydrocarbon responsiveness; aryl hydrocarbon hydroxylase; bHLHe76; dioxin receptor; inflammatory reactivity |
| Strain of Origin | Not Applicable |
| Chromosome | 12 |
| General Note | Strain of origin - this allele was found in DBA/2J, AKR/J, 129, SWR, RF, NZB strains |
| Molecular Note | This allele encodes a 104 kDa receptor that is stabilized by molybdate and has an affinity for ligand 10-100 fold lower than that of the receptor produced by the C57BL/6J allele. PCR sequencing of cDNA revealed ten nucleotide differences between the coding sequences of the DBA/2J and C57BL/6J receptors. Five of the ten differences would cause amino acid changes. One of these, an apparent T to C transition replaces the opal termination codon in the C57BL/6J allele with an arginine codon in the DBA/2J allele. This change would extend translation of the DBA/2J mRNA by 43 amino acids, accounting for the larger size of the peptide produced by this allele (104 kDa vs 95 kDa for the C57BL/6J allele). A second T to C transition changes a leucine codon in the C57BL/6J allele to a proline codon in the DBA/2J allele, and would likely change secondary structure of the peptide and thus ligand affinity. |
Del(9Cyp1a2-Cyp1a1)1Dwn
Allele Symbol: Del(9Cyp1a2-Cyp1a1)1Dwn
| Allele Name | deletion, Chr 9, Daniel W Nebert 1 |
|---|---|
| Allele Type | Targeted (Null/Knockout) |
| Allele Synonym(s) | Cyp1a1/1a2-; Cyp1a2/Cyp1a1tm2Dwn |
| Gene Symbol and Name | Del(9Cyp1a2-Cyp1a1)1Dwn, deletion, Chr 9, Daniel W Nebert 1 |
| Gene Synonym(s) | |
| Strain of Origin | 129P2/OlaHsd and 129S6/SvEvTac |
| Chromosome | 9 |
| Molecular Note | The Cyp1a2(t) targeted allele (J:122409) was generated using a targeting vector designed to insert a loxP-flanked PGK-NEO cassette 350 bp downstream of the endogenous stop codon (about 60 bp 3' of exon 7). The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells which were microinjection into C57BL/6 blastocysts. The chimeras were bred with C57BL/6 to generate Cyp1a2(t) mutant mice. To create the Cyp1a1 targeted allele (J:59398), a targeting vector was designed to insert a loxP-flanked hypoxanthine phosphoribosyltransferase (HPRT) minigene in intron 1 and a loxP site downstream of the termination codon in exon 7. Following electroporation into E14TG2a (HPRT-) ES cells and ES cell microinjection into the blastocoele cavity of C57BL/6J embryos, chimeric males were bred with C57BL/6J females. As described in J:86748, these Cyp1a1(t) mutant mice were then bred to a Cre-deleter strain (CAGGS-CRE, mixed C57BL/6J and FVB/NJ genetic background). The resulting transgenic mice found to be heterozygous for the floxed null Cyp1a1(-) allele (containing only exon 1, a portion of intron 1, and one remaining loxP site in the 3' UTR) were bred to mice heterozygous for the Cyp1a2(t) allele (as described in J:122409). Mutant mice (Cyp1a2(t), Cyp1a1(-), CAGGS-CRE) were bred to C57BL/6. Because of the close genomic position of these two genes, offspring having undergone Cre recombinase-mediated interchromosomal recombination between the loxP sites 3' beyond the stop codons of the Cyp1a2 and Cyp1a1 genes could be selected. Such mice were backcrossed to C57BL/6 for 10 generations (while selecting against the Cre-deleter transgene) to generate Cyp1a1/1a2 mutant mice. |
Tg(CYP1A1,CYP1A2)1Dwn
Allele Symbol: Tg(CYP1A1,CYP1A2)1Dwn
| Allele Name | transgene insertion 1, Daniel W Nebert |
|---|---|
| Allele Type | Transgenic (Humanized sequence, Inserted expressed sequence) |
| Allele Synonym(s) | hCYP1A1_1A2, BAC-H |
| Gene Symbol and Name | Tg(CYP1A1,CYP1A2)1Dwn, transgene insertion 1, Daniel W Nebert |
| Gene Synonym(s) | hCYP1A1_1A2, BAC-H |
| Promoter | CYP1A1, cytochrome P450 family 1 subfamily A member 1, human |
| Promoter | CYP1A2, cytochrome P450 family 1 subfamily A member 2, human |
| Expressed Gene | CYP1A1, cytochrome P450 family 1 subfamily A member 1, human |
| Expressed Gene | CYP1A2, cytochrome P450 family 1 subfamily A member 2, human |
| Strain of Origin | (C57BL/6J x DBA/2J)F1 |
| Chromosome | UN |
| Molecular Note | To create "humanized" CYP1A1 and CYP1A2 transgenic mice (hCYP1A1_1A2), a single copy of the 180-kb human CYP1A1_CYP1A2 locus-containing BAC-H (BAC Human CTB clone 31H21: including the 23.3 kb spacer region, 90 kb of CYP1A2 3'-flanking region and 53 kb of CYP1A1 3' flanking region) was microinjected into (C57BL/6J x DBA/2J)F1 oocytes. Founder mice having a single copy of BAC-H were identified and then backcrossed for 10 generations to C57BL/6 mice. |
Disease Terms
Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Research Areas By Genotype
This mouse can be used to support research in many areas including:
- Cancer Research
- Toxicology
- Cell Biology Research
- Transcriptional Regulation
- Research Tools
- Cancer Research
- Cell Biology Research
- Metabolism Research
- Toxicology Research
- drug metabolism
- drug/compound testing
Genotype: Ahrd related
- Metabolism Research
- Research Tools
- Toxicology Research
References
- Shi Z; Chen Y; Dong H; Amos-Kroohs RM; Nebert DW. 2008. Generation of a 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mouse line harboring the poor-affinity aryl hydrocarbon receptor. Biochem Biophys Res Commun 376(4):775-80. PubMed: 18814841MGI: J:141523
Additional - Ahrd related
- Benedict WF; Considine N; Nebert DW. 1973. Genetic differences in aryl hydrocarbon hydroxylase induction and benzo(a)pyrene-produced tumorigenesis in the mouse. Mol Pharmacol 9(2):266-77. PubMed: 4123113MGI: J:84312
- Boobis AR; Nebert DW. 1976. Genetic differences in the metabolism of carcinogens and in the binding of benzo (a) pyrene metabolites to DNA. Adv Enzyme Regul 15:339-62. PubMed: 1030186MGI: J:12156
- Castro DJ; Lohr CV; Fischer KA; Pereira CB; Williams DE. 2008. Lymphoma and lung cancer in offspring born to pregnant mice dosed with dibenzo[a,l]pyrene: the importance of in utero vs. lactational exposure. Toxicol Appl Pharmacol 233(3):454-8. PubMed: 18848954MGI: J:143604
- Chang C; Smith DR; Prasad VS; Sidman CL; Nebert DW; Puga A. 1993. Ten nucleotide differences, five of which cause amino acid changes, are associated with the Ah receptor locus polymorphism of C57BL/6 and DBA/2 mice. Pharmacogenetics 3(6):312-21. PubMed: 8148872MGI: J:17460
- Curran CP; Altenhofen E; Ashworth A; Brown A; Kamau-Cheggeh C; Curran M; Evans A; Floyd R; Fowler J; Garber H; Hays B; Kraemer S; Lang A; Mynhier A; Samuels A; Strohmaier C. 2012. Ahrd Cyp1a2(-/-) mice show increased susceptibility to PCB-induced developmental neurotoxicity. Neurotoxicology 33(6):1436-42. PubMed: 22935098MGI: J:225088
- Curran CP; Miller KA; Dalton TP; Vorhees CV; Miller ML; Shertzer HG; Nebert DW. 2006. Genetic differences in lethality of newborn mice treated in utero with coplanar versus non-coplanar hexabromobiphenyl. Toxicol Sci 89(2):454-64. PubMed: 16291824MGI: J:113285
- Felton JS; Nebert DW. 1975. Mutagenesis of certain activated carcinogens in vitro associated with genetically mediated increases in monooxygenase activity and cytochrome P 1-450. J Biol Chem 250(17):6769-78. PubMed: 808546MGI: J:5564
- Gielen JE; Goujon FM; Nebert DW. 1972. Genetic regulation of aryl hydrocarbon hydroxylase induction. II. Simple Mendelian expression in mouse tissues in vivo. J Biol Chem 247(4):1125-37. PubMed: 4110756MGI: J:84250
- Goujon FM; Nebert DW; Gielen JE. 1972. Genetic expression of aryl hydrocarbon hydroxylase induction. IV. Interaction of various compounds with different forms of cytochrome P-450 and the effect on benzo(a)pyrene metabolism in vitro. Mol Pharmacol 8(6):667-80. PubMed: 4118365MGI: J:84252
- Harper PA; Golas CL; Okey AB. 1991. Ah receptor in mice genetically nonresponsive for cytochrome P4501A1 induction: cytosolic Ah receptor, transformation to the nuclear binding state, and induction of aryl hydrocarbon hydroxylase by halogenated and nonhalogenated aromatic hydrocarbons in embryonic tissues and cells. Mol Pharmacol 40(5):818-26. PubMed: 1658612MGI: J:2134
- Kennedy GD; Nukaya M; Moran SM; Glover E; Weinberg S; Balbo S; Hecht SS; Pitot HC; Drinkwater NR; Bradfield CA. 2014. Liver tumor promotion by 2,3,7,8-tetrachlorodibenzo-p-dioxin is dependent on the aryl hydrocarbon receptor and TNF/IL-1 receptors. Toxicol Sci 140(1):135-43. PubMed: 24718703MGI: J:215349
- Kerley-Hamilton JS; Trask HW; Ridley CJ; Dufour E; Lesseur C; Ringelberg CS; Moodie KL; Shipman SL; Korc M; Gui J; Shworak NW; Tomlinson CR. 2012. Inherent and benzo[a]pyrene-induced differential aryl hydrocarbon receptor signaling greatly affects life span, atherosclerosis, cardiac gene expression, and body and heart growth in mice. Toxicol Sci 126(2):391-404. PubMed: 22228805MGI: J:183715
- Kouri RE; Rude TH; Joglekar R; Dansette PM; Jerina DM; Atlas SA; Owens IS; Nebert DW. 1978. 2,3,7,8-tetrachlorodibenzo-p-dioxin as cocarcinogen causing 3-methylcholanthrene-initiated subcutaneous tumors in mice genetically 'nonresponsive' at Ah locus. Cancer Res 38(9):2777-83. PubMed: 679184MGI: J:84318
- Levova K; Moserova M; Nebert DW; Phillips DH; Frei E; Schmeiser HH; Arlt VM; Stiborova M. 2012. NAD(P)H:quinone oxidoreductase expression in Cyp1a-knockout and CYP1A-humanized mouse lines and its effect on bioactivation of the carcinogen aristolochic acid I. Toxicol Appl Pharmacol 265(3):360-7. PubMed: 22982977MGI: J:192865
- Lew BJ; Manickam R; Lawrence BP. 2011. Activation of the aryl hydrocarbon receptor during pregnancy in the mouse alters mammary development through direct effects on stromal and epithelial tissues. Biol Reprod 84(6):1094-102. PubMed: 21270426MGI: J:173706
- Moriguchi T; Motohashi H; Hosoya T; Nakajima O; Takahashi S; Ohsako S; Aoki Y; Nishimura N; Tohyama C; Fujii-Kuriyama Y; Yamamoto M. 2003. Distinct response to dioxin in an arylhydrocarbon receptor (AHR)-humanized mouse. Proc Natl Acad Sci U S A 100(10):5652-7. PubMed: 12730383MGI: J:132380
- Nebert DW; Atlas SA; Guenthner TM; Kouri RE. 1978. The Ah locus: genetic regulation of the enzymes which metabolize polycyclic hydrocarbons and the risk of cancer. In: Polycyclic Hydrocarbons and Cancer: Chemistry, Molecular Biology and Environment. Academic Press, New York. MGI: J:30693
- Nebert DW; Considine N; Owens IS. 1973. Genetic expression of aryl hydrocarbon hydroxylase induction. VI. Control of other aromatic hydrocarbon-inducible mono-oxygenase activities at or near the same genetic locus. Arch Biochem Biophys 157(1):148-59. PubMed: 4716952MGI: J:84313
- Nebert DW; Gelboin HV. 1969. The in vivo and in vitro induction of aryl hydrocarbon hydroxylase in mammalian cells of different species, tissues, strains, and developmental and hormonal states. Arch Biochem Biophys 134(1):76-89. PubMed: 4981257MGI: J:84248
- Nebert DW; Gielen JE. 1972. Genetic regulation of aryl hydrocarbon hydroxylase induction in the mouse. Fed Proc 31(4):1315-25. PubMed: 4114109MGI: J:5282
- Nebert DW; Gielen JE; Goujon FM. 1972. Genetic expression of aryl hydrocarbon hydroxylase induction. 3. Changes in the binding of n-octylamine to cytochrome P-450. Mol Pharmacol 8(6):651-66. PubMed: 4118364MGI: J:84251
- Nebert DW; Goujon FM; Gielen JE. 1972. Aryl hydrocarbon hydroxylase induction by polycyclic hydrocarbons: simple autosomal dominant trait in the mouse. Nat New Biol 236(65):107-10. PubMed: 4502804MGI: J:84249
- Nebert DW; Jensen NM. 1979. Benzo[a]pyrene-initiated leukemia in mice. Association with allelic differences at the Ah locus. Biochem Pharmacol 28(1):149-51. PubMed: 758905MGI: J:6074
- Nebert DW; Jensen NM; Shinozuka H; Kunz HW; Gill TJ 3rd. 1982. The Ah phenotype. Survey of forty-eight rat strains and twenty inbred mouse strains. Genetics 100(1):79-87. PubMed: 7095422MGI: J:6809
- Nebert DW; Kon H. 1973. Genetic regulation of aryl hydrocarbon hydroxylase induction. V. Specific changes in spin state of cytochrome P 450 from genetically responsive animals. J Biol Chem 248(1):169-78. PubMed: 4348203MGI: J:84311
- Nebert DW; Robinson JR; Niwa A; Kumaki K; Poland AP. 1975. Genetic expression of aryl hydrocarbon hydroxylase activity in the mouse. J Cell Physiol 85(2 Pt 2 Suppl 1):393-414. PubMed: 1091656MGI: J:84317
- Niwa A; Kumaki K; Nebert DW; Poland AP. 1975. Genetic expression of aryl hydrocarbon hydroxylase activity in the mouse. Distinction between the 'responsive' homozygote and heterozygote at the Ah locus. Arch Biochem Biophys 166(2):559-64. PubMed: 1119809MGI: J:84316
- Oesch F; Morris N; Daly JW. 1973. Genetic expression of the induction of epoxide hydrase and aryl hydrocarbon hydroxylase activities in the mouse by phenobarbital or 3-methylcholanthrene. Mol Pharmacol 9(5):629-6. PubMed: 4788156MGI: J:25852
- Okey AB; Vella LM; Harper PA. 1989. Detection and characterization of a low affinity form of cytosolic Ah receptor in livers of mice nonresponsive to induction of cytochrome P1-450 by 3-methylcholanthrene. Mol Pharmacol 35(6):823-30. PubMed: 2543914MGI: J:27899
- Poel WE; Stanton D; Peters E; Wade HO. 1958. Comparative susceptibilities of seven inbred strains of mice to qualified applications of 3, 4-benzpyrene Proc Am Assoc Cancer Res 2:335. MGI: J:84245
- Poland A; Bradfield C. 1992. A brief review of the Ah locus. Tohoku J Exp Med 168(2):83-7. PubMed: 1339107MGI: J:12546
- Poland A; Glover E. 1990. Characterization and strain distribution pattern of the murine Ah receptor specified by the Ahd and Ahb-3 alleles. Mol Pharmacol 38(3):306-12. PubMed: 2169579MGI: J:34840
- Poland A; Glover E; Kende AS. 1976. Stereospecific, high affinity binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin by hepatic cytosol. Evidence that the binding species is receptor for induction of aryl hydrocarbon hydroxylase. J Biol Chem 251(16):4936-46. PubMed: 956169MGI: J:84247
- Poland A; Glover E; Taylor BA. 1987. The murine Ah locus: a new allele and mapping to chromosome 12. Mol Pharmacol 32(4):471-8. PubMed: 2823093MGI: J:8895
- Poland A; Palen D; Glover E. 1994. Analysis of the four alleles of the murine aryl hydrocarbon receptor. Mol Pharmacol 46(5):915-21. PubMed: 7969080MGI: J:22144
- Poland A; Teitelbaum P; Glover E; Kende A. 1989. Stimulation of in vivo hepatic uptake and in vitro hepatic binding of [125I]2-lodo-3,7,8-trichlorodibenzo-p-dioxin by the administration of agonist for the Ah receptor. Mol Pharmacol 36(1):121-7. PubMed: 2546046MGI: J:126377
- Poland AP; Glover E; Robinson JR; Nebert DW. 1974. Genetic expression of aryl hydrocarbon hydroxylase activity. Induction of monooxygenase activities and cytochrome P1-450 formation by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice genetically 'nonresponsive' to other aromatic hydrocarbons. J Biol Chem 249(17):5599-606. PubMed: 4370044MGI: J:84314
- Quintana FJ; Basso AS; Iglesias AH; Korn T; Farez MF; Bettelli E; Caccamo M; Oukka M; Weiner HL. 2008. Control of T(reg) and T(H)17 cell differentiation by the aryl hydrocarbon receptor. Nature 453(7191):65-71. PubMed: 18362915MGI: J:136052
- Robinson JR; Considine N; Nebert DW. 1974. Genetic expression of aryl hydrocarbon hydroxylase induction. Evidence for the involvement of other genetic loci. J Biol Chem 249(18):5851-9. PubMed: 4413562MGI: J:84315
- Schmid FA; Demetriades MS; Schabel FM 3rd; Tarnowski GS. 1967. Toxicity of several cancerigenic polycyclic hydrocarbons and other agents in AKR and C57BL-6 mice. Cancer Res 27(3):563-7. PubMed: 6021514MGI: J:84246
- Schmid FA; Elmer I; Tarnowski GS. 1969. Genetic determination of differential inflammatory reactivity and subcutaneous tumor susceptibility of AKR-J and C57BL-6J mice to 7,12-dimethylbenz- [a]anthracene. Cancer Res 29(8):1585-9. PubMed: 5807232MGI: J:34134
- Schmid FA; Pena RC; Robinson W; Tarnowski GS. 1967. Toxicity of intraperitoneal injections of 7, 12-dimethylbenz[a]anthracene in inbred mice. Cancer Res 27(3):558-62. PubMed: 6021513MGI: J:26440
- Schmidt JV; Carver LA; Bradfield CA. 1993. Molecular characterization of the murine Ahr gene. Organization, promoter analysis, and chromosomal assignment. J Biol Chem 268(29):22203-9. PubMed: 8408082MGI: J:15153
- Shivanna B; Zhang W; Jiang W; Welty SE; Couroucli XI; Wang L; Moorthy B. 2013. Functional deficiency of aryl hydrocarbon receptor augments oxygen toxicity-induced alveolar simplification in newborn mice. Toxicol Appl Pharmacol 267(3):209-17. PubMed: 23337360MGI: J:193493
- Simonian PL; Wehrmann F; Roark CL; Born WK; O'Brien RL; Fontenot AP. 2010. gammadelta T cells protect against lung fibrosis via IL-22. J Exp Med 207(10):2239-53. PubMed: 20855496MGI: J:165803
- Smith AG; Clothier B; Robinson S; Scullion MJ; Carthew P; Edwards R; Luo J; Lim CK; Toledano M. 1998. Interaction between iron metabolism and 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice with variants of the Ahr gene: a hepatic oxidative mechanism. Mol Pharmacol 53(1):52-61. PubMed: 9443932MGI: J:45850
- Stiborova M; Levova K; Barta F; Shi Z; Frei E; Schmeiser HH; Nebert DW; Phillips DH; Arlt VM. 2012. Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2. Toxicol Sci 125(2):345-58. PubMed: 22086975MGI: J:183662
- Tanos R; Murray IA; Smith PB; Patterson A; Perdew GH. 2012. Role of the ah receptor in homeostatic control of Fatty Acid synthesis in the liver. Toxicol Sci 129(2):372-9. PubMed: 22696238MGI: J:188164
- Taylor BA. 1971. Strain distribution and linkage tests of 7,12-dimethylbenzanthracene (DMBA) inflammatory response in mice. Life Sci I 10(19):1127-34. PubMed: 5132702MGI: J:5244
- Thomas PE; Hutton JJ; Taylor BA. 1973. Genetic relationship between aryl hydrocarbon hydroxylase inducibility and chemical carcinogen induced skin ulceration in mice. Genetics 74(4):655-9. PubMed: 4750810MGI: J:5387
- Thomas PE; Kouri RE; Hutton JJ. 1972. The genetics of aryl hydrocarbon hydroxylase induction in mice: a single gene difference between C57BL-6J and DBA-2J. Biochem Genet 6(2):157-68. PubMed: 4666754MGI: J:31977
- Thorgeirsson SS; Nebert DW. 1977. The Ah locus and the metabolism of chemical carcinogens and other foreign compounds. Adv Cancer Res 25:149-93. PubMed: 405846MGI: J:5822
- Walisser JA; Bunger MK; Glover E; Bradfield CA. 2004. Gestational exposure of Ahr and Arnt hypomorphs to dioxin rescues vascular development. Proc Natl Acad Sci U S A 101(47):16677-82. PubMed: 15545609MGI: J:94465
- Williams EG; Mouchiroud L; Frochaux M; Pandey A; Andreux PA; Deplancke B; Auwerx J. 2014. An evolutionarily conserved role for the aryl hydrocarbon receptor in the regulation of movement. PLoS Genet 10(9):e1004673. PubMed: 25255223MGI: J:232452
- Yeager RL; Reisman SA; Aleksunes LM; Klaassen CD. 2009. Introducing the 'TCDD-inducible AhR-Nrf2 gene battery'. Toxicol Sci 111(2):238-46. PubMed: 19474220MGI: J:154083
- Yu Z; Mahadevan B; Lohr CV; Fischer KA; Louderback MA; Krueger SK; Pereira CB; Albershardt DJ; Baird WM; Bailey GS; Williams DE. 2006. Indole-3-carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon dibenzo[a,l]pyrene. Carcinogenesis 27(10):2116-23. PubMed: 16704990MGI: J:113356
- Zhou Y; Tung HY; Tsai YM; Hsu SC; Chang HW; Kawasaki H; Tseng HC; Plunkett B; Gao P; Hung CH; Vonakis BM; Huang SK. 2013. Aryl hydrocarbon receptor controls murine mast cell homeostasis. Blood 121(16):3195-204. PubMed: 23462117MGI: J:197552
Additional - Del(9Cyp1a2-Cyp1a1)1Dwn related
- Bansal S; Leu AN; Gonzalez FJ; Guengerich FP; Chowdhury AR; Anandatheerthavarada HK; Avadhani NG. 2014. Mitochondrial targeting of cytochrome P450 (CYP) 1B1 and its role in polycyclic aromatic hydrocarbon-induced mitochondrial dysfunction. J Biol Chem 289(14):9936-51. PubMed: 24497629MGI: J:212446
- Cheung C; Loy S; Li GX; Liu AB; Yang CS. 2011. Rapid induction of colon carcinogenesis in CYP1A-humanized mice by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate. Carcinogenesis 32(2):233-9. PubMed: 21081470MGI: J:168018
- Dalton TP; Dieter MZ; Matlib RS; Childs NL; Shertzer HG; Genter MB; Nebert DW. 2000. Targeted knockout of Cyp1a1 gene does not alter hepatic constitutive expression of other genes in the mouse [Ah] battery. Biochem Biophys Res Commun 267(1):184-9. PubMed: 10623596MGI: J:59398
- Divanovic S; Dalli J; Jorge-Nebert LF; Flick LM; Galvez-Peralta M; Boespflug ND; Stankiewicz TE; Fitzgerald JM; Somarathna M; Karp CL; Serhan CN; Nebert DW. 2013. Contributions of the three CYP1 monooxygenases to pro-inflammatory and inflammation-resolution lipid mediator pathways. J Immunol 191(6):3347-57. PubMed: 23956430MGI: J:205875
- Dragin N; Shi Z; Madan R; Karp CL; Sartor MA; Chen C; Gonzalez FJ; Nebert DW. 2008. Phenotype of the Cyp1a1/1a2/1b1-/- triple-knockout mouse. Mol Pharmacol 73(6):1844-56. PubMed: 18372398MGI: J:153792
- Dragin N; Uno S; Wang B; Dalton TP; Nebert DW. 2007. Generation of 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-) mouse line. Biochem Biophys Res Commun 359(3):635-42. PubMed: 17560947MGI: J:122409
- Iqbal J; Sun L; Cao J; Yuen T; Lu P; Bab I; Leu NA; Srinivasan S; Wagage S; Hunter CA; Nebert DW; Zaidi M; Avadhani NG. 2013. Smoke carcinogens cause bone loss through the aryl hydrocarbon receptor and induction of Cyp1 enzymes. Proc Natl Acad Sci U S A 110(27):11115-20. PubMed: 23776235MGI: J:198711
- Levova K; Moserova M; Nebert DW; Phillips DH; Frei E; Schmeiser HH; Arlt VM; Stiborova M. 2012. NAD(P)H:quinone oxidoreductase expression in Cyp1a-knockout and CYP1A-humanized mouse lines and its effect on bioactivation of the carcinogen aristolochic acid I. Toxicol Appl Pharmacol 265(3):360-7. PubMed: 22982977MGI: J:192865
- Li G; Wang H; Liu AB; Cheung C; Reuhl KR; Bosland MC; Yang CS. 2012. Dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced prostate carcinogenesis in CYP1A-humanized mice. Cancer Prev Res (Phila) 5(7):963-72. PubMed: 22581815MGI: J:231719
- Stiborova M; Levova K; Barta F; Shi Z; Frei E; Schmeiser HH; Nebert DW; Phillips DH; Arlt VM. 2012. Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2. Toxicol Sci 125(2):345-58. PubMed: 22086975MGI: J:183662
- Takahashi S; Fukami T; Masuo Y; Brocker CN; Xie C; Krausz KW; Wolf CR; Henderson CJ; Gonzalez FJ. 2016. Cyp2c70 is responsible for the species difference in bile acid metabolism between mice and humans. J Lipid Res 57(12):2130-2137. PubMed: 27638959MGI: J:237369
- Uno S; Wang B; Shertzer HG; Nebert DW; Dalton TP. 2003. Balancer-Cre transgenic mouse germ cells direct the incomplete resolution of a tri-loxP-targeted Cyp1a1 allele, producing a conditional knockout allele. Biochem Biophys Res Commun 312(2):494-9. PubMed: 14637164MGI: J:86748
Additional - Tg(CYP1A1,CYP1A2)1Dwn related
- Chen JX; Li G; Wang H; Liu A; Lee MJ; Reuhl K; Suh N; Bosland MC; Yang CS. 2016. Dietary tocopherols inhibit PhIP-induced prostate carcinogenesis in CYP1A-humanized mice. Cancer Lett 371(1):71-8. PubMed: 26582657MGI: J:237564
- Cheung C; Loy S; Li GX; Liu AB; Yang CS. 2011. Rapid induction of colon carcinogenesis in CYP1A-humanized mice by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate. Carcinogenesis 32(2):233-9. PubMed: 21081470MGI: J:168018
- Dragin N; Uno S; Wang B; Dalton TP; Nebert DW. 2007. Generation of 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-) mouse line. Biochem Biophys Res Commun 359(3):635-42. PubMed: 17560947MGI: J:122409
- Jiang Z; Dalton TP; Jin L; Wang B; Tsuneoka Y; Shertzer HG; Deka R; Nebert DW. 2005. Toward the evaluation of function in genetic variability: characterizing human SNP frequencies and establishing BAC-transgenic mice carrying the human CYP1A1_CYP1A2 locus. Hum Mutat 25(2):196-206. PubMed: 15643613MGI: J:96716
- Levova K; Moserova M; Nebert DW; Phillips DH; Frei E; Schmeiser HH; Arlt VM; Stiborova M. 2012. NAD(P)H:quinone oxidoreductase expression in Cyp1a-knockout and CYP1A-humanized mouse lines and its effect on bioactivation of the carcinogen aristolochic acid I. Toxicol Appl Pharmacol 265(3):360-7. PubMed: 22982977MGI: J:192865
- Li G; Wang H; Liu AB; Cheung C; Reuhl KR; Bosland MC; Yang CS. 2012. Dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced prostate carcinogenesis in CYP1A-humanized mice. Cancer Prev Res (Phila) 5(7):963-72. PubMed: 22581815MGI: J:231719
- Stiborova M; Levova K; Barta F; Shi Z; Frei E; Schmeiser HH; Nebert DW; Phillips DH; Arlt VM. 2012. Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2. Toxicol Sci 125(2):345-58. PubMed: 22086975MGI: J:183662
- Takahashi S; Fukami T; Masuo Y; Brocker CN; Xie C; Krausz KW; Wolf CR; Henderson CJ; Gonzalez FJ. 2016. Cyp2c70 is responsible for the species difference in bile acid metabolism between mice and humans. J Lipid Res 57(12):2130-2137. PubMed: 27638959MGI: J:237369
- Wang H; Liu A; Kuo Y; Chi E; Yang X; Zhang L; Yang CS. 2016. Obesity promotes PhIP-induced small intestinal carcinogenesis in hCYP1A-db/db mice: involvement of mutations and DNA hypermethylation of Apc. Carcinogenesis 37(7):723-30. PubMed: 27207656MGI: J:232782