These transgenic mice, expressing the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse prion protein promoter exhibit an increase in expression of ABCA1 in the cortex and reduction of apoE levels in the hippocampus and cerebrospinal fluid. These mice may be useful in studies of amyloid deposition in the brain and Alzheimers disease.
Dr. David M. Holtzman, Washington University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter.
These founder line E mice have a 6-fold increase in expression of ABCA1 in the cortex over wild-type levels. Trangene expression is high in total brain tissue, kidney, testis and muscle as detected by Western blot analysis. Transgenic mice exhibit reduced apoE levels: 40% of wild-type levels in the hippocampus, approximately half of wild-type levels in cerebrospinal fluid (CSF). The apoE protein that is overexpressed has altered biochemical properties and is not as soluble as that found in controls. Lipoprotein particles from the CSF that contain apoE protein are larger in size than wild-type, indicating that the transgenic apoE particles are more lipidated.
Male transgenic mice have atropied testes, defective spermatogenesis and are infertile. The strain can be maintained by mating hemizygous females to wild-type males.
These mice may be useful in studies of the underlying mechanism of amyloid deposition in the brain and Alzheimers disease.
A transgenic construct containing the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter was injected into fertilized B6CBAF1 eggs. Founder line E was subsequently established. The mice were backcrossed to C57BL/6 (see SNP notes below) for four generations. After arriving at The Jackson Laboratory, the mice were backcrossed to C57BL/6J for one generation.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | Abca1, ATP-binding cassette, sub-family A (ABC1), member 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion E, David M Holtzman |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | PrP-mAbca1 line E |
Gene Symbol and Name | Tg(Prnp-Abca1)EHol, transgene insertion E, David M Holtzman |
Gene Synonym(s) | |
Promoter | Prnp, prion protein, mouse, laboratory |
Expressed Gene | Abca1, ATP-binding cassette, sub-family A (ABC1), member 1, mouse, laboratory |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter was injected into fertilized B6CBAF1 eggs. Founder line E was subsequently established. These founder line E mice have a 6-fold increase in expression of ABCA1 in the cortex over wildtype levels. Transgene expression is high in total brain tissue, kidney, testis and muscle as detected by Western blot analysis. |
Mutations Made By | Dr. David Holtzman, Washington University |
When maintaining a live colony, these transgenic mice can be bred by mating female hemizygote to a wildtype male. Male transgenic mice are sterile.
When using the PrP-mAbca1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008596 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous females and wildtype males for Tg(Prnp-Abca1)EHol |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $3373.50 |
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