C57BL/6-Ackr3^tm1Litt/J

Stock number: 008591
Research areas: Cardiovascular Research, Developmental Biology Research, Research Tools

Description

This targeted mutation strain carries GFP knocked into the chemokine (C-X-C motif) receptor 7 gene. Fluorescently labeled T and B cells can be found in the lymphoid organs (spleen, small intestine, and large intestine) of heterozygous animals. Homozygous animals die during the perinatal period and have ventricular septal defects and semilunar valve malformations. This strain may be useful in immunological studies as well as studies of cardiac development.

Development

GFP (green fluorescent protein) cDNA was knocked into the second exon of the gene immediately after the endogenous start codon. A loxP-flanked neomycin resistance cassette was also inserted right after the GFP sequence. The targeting vector was introduced to an embryonic stem cell line derived from albino C57BL/6 mice. A cross with a C57BL/6 background EIIa-Cre mouse excised the neomycin cassette. This line was maintained on a C57BL/6 background by the donating investigator.

Allele

Symbol: Ackr3^tm1Litt
Name: targeted mutation 1, Dan R Littman
MGI accession ID: MGI:3801080
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Ackr3
Marker name: atypical chemokine receptor 3
Chromosome: 1
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP labels T and B cells in lymphoid organs (spleen, small intestine, and large intestine) of heterozygous animals.
Molecular note: EGFP (enhanced green fluorescent protein) cDNA was knocked into the second exon of the gene immediately after the endogenous start codon. A loxP-flanked neomycin resistance cassette was also inserted right after the EGFP sequence. The targeting vector was introduced to an embryonic stem cell line derived from albino C57BL/6 mice. A cross with a C57BL/6 background EIIa-Cre mouse excised the neomycin cassette.