This targeted mutation strain carries GFP knocked into the chemokine (C-X-C motif) receptor 7 gene. Fluorescently labeled T and B cells can be found in the lymphoid organs (spleen, small intestine, and large intestine) of heterozygous animals. Homozygous animals die during the perinatal period and have ventricular septal defects and semilunar valve malformations. This strain may be useful in immunological studies as well as studies of cardiac development.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Ackr3 | atypical chemokine receptor 3 |
GFP (green fluorescent protein) cDNA was knocked into the second exon of the gene immediately after the endogenous start codon. A loxP-flanked neomycin resistance cassette was also inserted right after the GFP sequence. The targeting vector was introduced to an embryonic stem cell line derived from albino C57BL/6 mice. A cross with a C57BL/6 background EIIa-Cre mouse excised the neomycin cassette. This line was maintained on a C57BL/6 background by the donating investigator.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP labels T and B cells in lymphoid organs (spleen, small intestine, and large intestine) of heterozygous animals. |
Allele Name | targeted mutation 1, Dan R Littman |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | CXCR7GFP; Cxcr7tm1(GFP)Litt |
Gene Symbol and Name | Ackr3, atypical chemokine receptor 3 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP labels T and B cells in lymphoid organs (spleen, small intestine, and large intestine) of heterozygous animals. |
Strain of Origin | C57BL/6 |
Chromosome | 1 |
Molecular Note | EGFP (enhanced green fluorescent protein) cDNA was knocked into the second exon of the gene immediately after the endogenous start codon. A loxP-flanked neomycin resistance cassette was also inserted right after the EGFP sequence. The targeting vector was introduced to an embryonic stem cell line derived from albino C57BL/6 mice. A cross with a C57BL/6 background EIIa-Cre mouse excised the neomycin cassette. |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
When maintained as a live colony, heterozygotes may be bred. Homozygotes die during the perinatal period.
When using the C57BL/6-Ackr3tm1Litt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008591 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Cxcr7<tm1Litt> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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