B6.129P2-Card11^tm1Litt/J

Stock number: 008588
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Mice that are homozygous for this Card11 (caspase recruitment domain family, member 11) targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Development of subpopulations of B and T lymphocytes and activation of B and T lymphocytes following antigen-receptor stimulation are impaired. This mutant mouse strain may be useful in studies of immune response and transplantation studies.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA and protein) is detected by Northern and Western blot analysis of splenocytes isolated from homozygous animals. Development of subpopulations of B and T lymphocytes and activation of B and T lymphocytes following antigen-receptor stimulation are impaired. This mutant mouse strain may be useful in studies of immune response and transplantation studies.

Development

A targeting vector was used to place a loxP site in intron 2 and a loxP-flanked neomycin resistance cassette (in opposite transcriptional orientation) in intron 4. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, then backcrossed to the same for 14 generations.

Allele

Symbol: Card11^tm1Litt
Name: targeted mutation 1, Dan R Littman
MGI accession ID: MGI:3039682
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Card11
Marker name: caspase recruitment domain family, member 11
Chromosome: 5
Strain of origin: 129P2/OlaHsd
Molecular note: A single loxP site was inserted into intron 2 and a loxP-flanked neomycin selection cassette was inserted into intron 4 through homologous recombination. Western blot analysis on extracts derived from splenocytes of homozygous mice confirmed that no detectable protein was expressed from this allele.