Mice that are homozygous for this Card11 (caspase recruitment domain family, member 11) targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Development of subpopulations of B and T lymphocytes and activation of B and T lymphocytes following antigen-receptor stimulation are impaired. This mutant mouse strain may be useful in studies of immune response and transplantation studies.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Card11 | caspase recruitment domain family, member 11 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA and protein) is detected by Northern and Western blot analysis of splenocytes isolated from homozygous animals. Development of subpopulations of B and T lymphocytes and activation of B and T lymphocytes following antigen-receptor stimulation are impaired. This mutant mouse strain may be useful in studies of immune response and transplantation studies.
A targeting vector was used to place a loxP site in intron 2 and a loxP-flanked neomycin resistance cassette (in opposite transcriptional orientation) in intron 4. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, then backcrossed to the same for 14 generations.
Allele Name | targeted mutation 1, Dan R Littman |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CARMA1-; carma1FN |
Gene Symbol and Name | Card11, caspase recruitment domain family, member 11 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 5 |
Molecular Note | A single loxP site was inserted into intron 2 and a loxP-flanked neomycin selection cassette was inserted into intron 4 through homologous recombination. Western blot analysis on extracts derived from splenocytes of homozygous mice confirmed that no detectable protein was expressed from this allele. |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
When maintained as a live colony, heterozygotes may be bred.
When using the B6.129P2-Card11tm1Litt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008588 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Card11<tm1Litt> |
Frozen Mouse Embryo | B6.129P2-Card11<tm1Litt>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Card11<tm1Litt>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Card11<tm1Litt>/J | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Card11<tm1Litt>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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