Mice homozygous for this G protein-coupled receptor 132 (Gpr132, G2A) targeted mutation demonstrate a normal pattern of T and B lineage differentiation through young adulthood when they are indistinguishable from wild-type littermates. As they age, they develop secondary lymphoid organ enlargement associated with abnormal expansion of both T and B lymphocytes. Homozygotes greater than one year old develop a slowly progressive wasting syndrome, characterized by lymphocytic infiltration into various tissues, glomerular immune complex deposition, and anti-nuclear autoantibodies. This strain represents a novel model of late-onset autoimmune syndrome.
Owen Witte, University of California, Los Angeles
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Gpr132 | G protein-coupled receptor 132 |
Mice homozygous for this targeted mutation demonstrate a normal pattern of T and B lineage differentiation through young adulthood when they are indistinguishable from wildtype littermates. As they age, they develop secondary lymphoid organ enlargement associated with abnormal expansion of both T and B lymphocytes. Homozygotes greater than one year old develop a slowly progressive wasting syndrome, characterized by lymphocytic infiltration into various tissues, glomerular immune complex deposition, and anti-nuclear autoantibodies. T cells are hyperresponsive to TCR stimulation, exhibiting enhanced proliferation and a lower threshold of activation. RT-PCR analysis of RNA from homozygous pre-B and T cells does not detect mRNA. The lacZ marker incorporated in the targeted mutation is expressed in lymphoid tissues like bone marrow, spleen and thymus. Expression of lacZ in the testis (primarily mature spermatids) and the cortical-medullary junction of the kidney have also been observed. Low levels of lacZ expression are seen in lymphoid organs of heterozygotes. This strain represents a novel model of late-onset autoimmune syndrome.
A targeting vector was constructed to replace the second exon with an IRES beta galactosidase (LacZ) ane PGK-neomycin resistance cassette. The vector was electroporated into 129X1/SvJ-derived embryonic stem cells. Appropriately-targeted cells were injected into C57BL/6 blastocysts. Chimeric males were crossed with BALB/c females, then backcrossed to C57BL/6 for 12 generations.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression |
Allele Name | targeted mutation 1, Owen N Witte |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | G2A- |
Gene Symbol and Name | Gpr132, G protein-coupled receptor 132 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129X1/SvJ |
Chromosome | 12 |
Molecular Note | The gene was disrupted by replacement of exon 2 (98% of the total coding sequence) with an IRES-lacZ-PGK-neo cassette. Absence of gene expression was confirmed by RT-PCR analysis of pre-B and -T cells from homozygous mutant animals. |
Mutations Made By | Owen Witte, University of California, Los Angeles |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129X1(C)-Gpr132tm1Witt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008576 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gpr132<tm1Witt> |
Frozen Mouse Embryo | B6.129X1(C)-Gpr132<tm1Witt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1(C)-Gpr132<tm1Witt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1(C)-Gpr132<tm1Witt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1(C)-Gpr132<tm1Witt>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.