B6(CAST)-Prkra^lear/GrsrJ

Stock number: 008568
Product name: little ears
Research areas: Developmental Biology Research

Description

Homozygous mutants are characterized by reduced body size and ear pinna.

Detailed description

Homozygotes have an overall reduced body size, smaller than normal ear pinnae, hearing loss, and reduced fertility.

Development

The little ears mutation arose spontaneously in the C57BL/6J sequence of a B6.CAST congenic research strain used for mapping a QTL. This spontaneous mutation was backcrossed twice onto C57BL/6J and then maintained by sibling intercross of primarily heterozygotes and homozygotes. In 2010 this strain reached generation +N2F4.

Allele

Symbol: Prkra^lear
Name: little ears
MGI accession ID: MGI:4442387
Generation method: Spontaneous
Marker symbol: Prkra
Marker name: protein kinase, interferon inducible double stranded RNA dependent activator
Chromosome: 2
Strain of origin: C57BL/6J
Molecular note: This spontaneous mutation has a T-to-A transversion at chromosome 2:76,639,197 (GRCm38) in intron 5 immediately adjacent to the conserved splice donor site.