NOD.Cg-B2m^tm1Unc Tg(GFAP-B2m)9Mdos/J

Stock number: 008542
Product name: NOD.GFAP-B2m
Strain synonyms: NOD.GFAP-B2m
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Metabolism Research, Research Tools

Description

This GFAP-B2m transgenic, B2m deficient strain expresses the GFAP-B2m transgene in astrocytes and peri-islet Schwann cells. This strain is resistant to spontaneous diabetes, yet when MHC class 1 is restored in adoptive transfer studies diabetes is accelerated. These mice are useful to study the role of neuronal autoantigen GFAP in autoimmunity and immunotherapy development.

Detailed description

Mice carrying the GFAP-B2m transgene and homozygous for the B2m^tm1Unc allele, commonly referred to as GFAP-B2m, are viable, fertile and diabetes resistant. Western blot analysis and histological analysis of brain and pancreas confirmed transgene expression in the astrocytes and peri-islet Schwann cells. FACs analysis indicates the absence of CD8+ T cells in the spleens of both NOD.GFAP-B2m and NOD.B2m deficient mice. Similar to the NOD.B2m deficient mice, NOD.GFAP-B2m mice do not develop spontaneous diabetes by forty weeks of age. In adoptive transfer experiments NOD.GFAP-B2m mice receiving splenocytes from diabetic NOD females develop diabetes at a significantly faster rate and higher incidence than non-transgenic littermates. No diabetes occurred in NOD.GFAP-B2m mice receiving splenocytes from AI4 TCR transgenic mice.

This strain is useful for studying the role of GFAP in autoimmunity and the development of immunotherapies.

Development

The transgene consists of the human glial fibrillary acidic protein, GFAP, promoter fused to murine Beta-2 microglobulin, B2m. Six founders resulted from the microinjection of the GFAP-B2m construct into NOD/LtJ oocytes. Progeny of founder line 9 were mated with NOD.129P2(B6)-B2m^tm1Unc/J (Stock No. 002309). In 2009, the Type 1 Diabetes Resource received NOD mice homozygous for the B2m allele and hemizygous for the GFAP-B2m transgene.

Allele

Symbol: Tg(GFAP-B2m)9Mdos
Name: transgene insertion 9, H Michael Dosch
MGI accession ID: MGI:3790767
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(GFAP-B2m)9Mdos
Marker name: transgene insertion 9, H Michael Dosch
Chromosome: UN
Strain of origin: NOD/ShiLtJ
Expressed genes: B2m,beta-2 microglobulin,mouse, laboratory
Molecular note: The transgene consists of the human GFAP promoter fused to murine Beta-2 microglobulin, B2m. Six founders resulted from the microinjection of the GFAP-B2m construct into NOD/LtJ oocytes. Founder Western blot analysis and histological analysis of brain, specifically astrocytes and pancreas, specifically the peri-islet Schwann cells, from transgenic animals confirmed transgene expression.

Allele

Symbol: B2m^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857133
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: B2m
Marker name: beta-2 microglobulin
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: Insertion of a neomycin-resistance gene into the second exon.