This GFAP-B2m transgenic, B2m deficient strain expresses the GFAP-B2m transgene in astrocytes and peri-islet Schwann cells. This strain is resistant to spontaneous diabetes, yet when MHC class 1 is restored in adoptive transfer studies diabetes is accelerated. These mice are useful to study the role of neuronal autoantigen GFAP in autoimmunity and immunotherapy development.
Hans-Michael Dosch, University of Toronto
Genetic Background | Generation |
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001976 NOD/ShiLtJ |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | B2m | beta-2 microglobulin |
Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice carrying the GFAP-B2m transgene and homozygous for the B2mtm1Unc allele, commonly referred to as GFAP-B2m, are viable, fertile and diabetes resistant. Western blot analysis and histological analysis of brain and pancreas confirmed transgene expression in the astrocytes and peri-islet Schwann cells. FACs analysis indicates the absence of CD8+ T cells in the spleens of both NOD.GFAP-B2m and NOD.B2m deficient mice. Similar to the NOD.B2m deficient mice, NOD.GFAP-B2m mice do not develop spontaneous diabetes by forty weeks of age. In adoptive transfer experiments NOD.GFAP-B2m mice receiving splenocytes from diabetic NOD females develop diabetes at a significantly faster rate and higher incidence than non-transgenic littermates. No diabetes occurred in NOD.GFAP-B2m mice receiving splenocytes from AI4 TCR transgenic mice.
This strain is useful for studying the role of GFAP in autoimmunity and the development of immunotherapies.
The transgene consists of the human glial fibrillary acidic protein, GFAP, promoter fused to murine Beta-2 microglobulin, B2m. Six founders resulted from the microinjection of the GFAP-B2m construct into NOD/LtJ oocytes. Progeny of founder line 9 were mated with NOD.129P2(B6)-B2mtm1Unc/J (Stock No. 002309). In 2009, the Type 1 Diabetes Resource received NOD mice homozygous for the B2m allele and hemizygous for the GFAP-B2m transgene.
Expressed Gene | B2m, beta-2 microglobulin, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | b2mnull; beta2M-; beta2m0; beta2mnull; beta2mo; beta2mtm1Unc; beta2MKO; beta2-m-KO; I0; MHC-I- |
Gene Symbol and Name | B2m, beta-2 microglobulin |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | Insertion of a neomycin-resistance gene into the second exon. |
Mutations Made By | Dr. Oliver Smithies, University of North Carolina at Chapel Hill |
Allele Name | transgene insertion 9, H Michael Dosch |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | GFAP-B2m |
Gene Symbol and Name | Tg(GFAP-B2m)9Mdos, transgene insertion 9, H Michael Dosch |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | B2m, beta-2 microglobulin, mouse, laboratory |
Strain of Origin | NOD/ShiLtJ |
Chromosome | UN |
Molecular Note | The transgene consists of the human GFAP promoter fused to murine Beta-2 microglobulin, B2m. Six founders resulted from the microinjection of the GFAP-B2m construct into NOD/LtJ oocytes. Founder Western blot analysis and histological analysis of brain, specifically astrocytes and pancreas, specifically the peri-islet Schwann cells, from transgenic animals confirmed transgene expression. |
Mutations Made By | Hans-Michael Dosch, University of Toronto |
TJL breeding scheme: homozygous for the B2m mutation and Tg/? x same Mice available for distribution: homozygous for the B2m mutation and Tg/? Breeder pairs available:homozygous for the B2m mutation and Tg/? x same
When using the NOD.GFAP-B2m mouse strain in a publication, please cite the originating article(s) and include JAX stock #008542 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for B2m<tm1Unc>, Hemizygous for Tg(GFAP-B2m)9Mdos |
Frozen Mouse Embryo | NOD.Cg-B2m<tm1Unc> Tg(GFAP-B2m)9Mdos/J | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-B2m<tm1Unc> Tg(GFAP-B2m)9Mdos/J | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-B2m<tm1Unc> Tg(GFAP-B2m)9Mdos/J | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-B2m<tm1Unc> Tg(GFAP-B2m)9Mdos/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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