These transgenic mice express the Cre recombinase under the control of the mouse chondroitin sulfate proteoglycan 4 (Cspg4) promoter/enhancer. Cre recombinase expression is detected in Cspg4 expressing glial cells and vasculature throughout the brain as well as in Cspg4-expressing cells in other tissues from late embryonic stages (~embryonic day 14) throughout adulthood. These transgenic mice may be may be crossed to various floxed mutants to delete floxed sequences specifically in Cspg4-expressing cells.
Akiko Nishiyama, University of Connecticut
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These transgenic mice express Cre recombinase under the control of the mouse chondroitin sulfate proteoglycan 4 (Cspg4) promoter/enhancer. The transgene integrated into an intron of Ifi208 (interferon activated gene 208) of chromosome 1. Founder line 1 has a copy number of 1-3. Mice hemizygous for the NG2creBAC transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre recombinase expression is detected in NG2 expressing glial cells and vasculature throughout the brain as well as in NG2-expressing cells in other tissues from late embryonic stages (~embryonic day 14) throughout adulthood. The efficiency of Cre-mediated recombination is less than 100% (86% in forebrain and 70-75% in spinal cord). Immunoreactive Cre was not detected in S100beta+ or GFAP+ astrocytes or APC+ oligodendrocytes. These NG2creBAC transgenic mice may be may be crossed to various floxed mutants to delete floxed sequences specifically in NG2-expressing cells. The donating investigator reports that there appears to be transient Cre expression in some projection neurons and interneurons scattered throughout the cerebral cortex, hippocampus, spinal cord, and other regions after postnatal day 30 and in male germ cells.
Additional Note: This strain needs to be monitored by quantitative PCR specific for cre. Breeding through the male germline leads to copy number loss and poor expression. It is maintained by breeding carrier females with noncarrier or C57BL/6J males only.
A 208 kb RPCI-23 C57BL/6J mouse bacterial artificial chromosome (BAC) containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences) was modified by inserting a cre recombinase coding sequence with a nuclear localization signal followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the Cre recombinase sequence. DNA from a correctly modified BAC clone was used as the source for the NG2creBAC transgene was microinjected into the pronucleus of fertilized oocytes from C57BL/6J mice. Founder mice were subsequently established. The transgene integrated into an intron of Ifi208 (interferon activated gene 208) of chromosome 1. Founder line 1 has a copy number of 1-3. The transgenic mice were maintained on a mixed C57BL/6 and FVB genetic background prior to arrival at The Jackson Laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | NG2 expressing glial cells and vasculature throughout the brain as well as in NG2-expressing cells in other tissues from late embryonic stages (~embryonic day 14) throughout adulthood |
Allele Name | transgene insertion 1, Akiko Nishiyama |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Cspg4(NG2)-Cre; ng2-Cre; NG2creBAC; Tg(Cspg4-cre)1Akik |
Gene Symbol and Name | Ifi208, interferon activated gene 208 |
Gene Synonym(s) | |
Promoter | Cspg4, chondroitin sulfate proteoglycan 4, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | NG2 expressing glial cells and vasculature throughout the brain as well as in NG2-expressing cells in other tissues from late embryonic stages (~embryonic day 14) throughout adulthood |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | 1 |
Molecular Note | The transgene construct consisted of the 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), which was modified by inserting an nls-cre coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the cre sequence. Cre expression is detected only in Cspg4-expressing cells at P1, P14 and P60 and not in Gfap +ve astrocytes or APC +ve oligodendrocytes. Activity is also detected in male germ cells. Line 1 inserted into an intron of the gene at 173692115 (Build GRCm38/mm10). Founder line 1 has a copy number of 1-3. |
Mutations Made By | Akiko Nishiyama, University of Connecticut |
When maintaining a live colony, transgenic females may be bred to wildtype (noncarrier) male siblings. The donating investigator reports that NG2creBAC transgene expression may be more stable when passed through the female germline (i.e. transgenic females are bred with noncarrier males). Also, Cre recombinase may be expressed in male germ cells. Breeding through the male germline leads to copy number loss and poor expression.
When using the B6;FVB-Ifi208Tg(Cspg4-cre)1Akik/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008533 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous Females and non-carrier for Tg(Cspg4-cre)1Akik minimum of 1 pair |
Frozen Mouse Embryo | B6;FVB-Ifi208<Tg(Cspg4-cre)1Akik>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Ifi208<Tg(Cspg4-cre)1Akik>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Ifi208<Tg(Cspg4-cre)1Akik>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Ifi208<Tg(Cspg4-cre)1Akik>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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