When these Ngn1-CreERT2 transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Neurog1 expressing cells such as neurons in the cortex, hippocampus, thalamus, hypothalamus and the cochlear-vestibular ganglion. This mutant mouse strain may be useful for studying neuron development and lineage mapping of Neurog1 expressing cells.
Lisa Goodrich, Harvard Medical School
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 1, Neurog1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the cortex, hippocampus, thalamus, hypothalamus and cochlear-vestibular ganglion. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. This mutant mouse strain may be useful for studying neuron development and lineage mapping of Neurog1 expressing cells.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting construct containing an FRT site flanked PGK-Neo cassette and sequence encoding cre/ESR1 (CreERT2) was inserted into the single coding exon of the gene in a BAC. The PGK-neo cassette was removed by transient Flp-recombinase expression. The resulting BAC transgene was microinjected into C57BL/6 donor eggs. Founder line 7 was subsequently established. The mice were then crossed to B6129PF1/J for five generations.
Expressed Gene | ESR1, estrogen receptor 1, human |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre is expressed in neurons in the cortex, hippocampus, thalamus, hypothalamus and the cochlear-vestibular ganglion. |
Allele Name | transgene insertion 1, Lisa V Goodrich |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Neurog1CreER; Ngn1CreER; Ngn1-CreERT2; Ngn-CreERT2; Tg(Neurog1-cre/ESR1)1Good |
Gene Symbol and Name | Tg(Neurog1-cre/ERT2)1Good, transgene insertion 1, Lisa V Goodrich |
Gene Synonym(s) | |
Promoter | Neurog1, neurogenin 1, mouse, laboratory |
Expressed Gene | ESR1, estrogen receptor 1, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre is expressed in neurons in the cortex, hippocampus, thalamus, hypothalamus and the cochlear-vestibular ganglion. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A transgene was constructed where cre recombinase fused to the ligand binding domain of a mutant human estrogen receptor was placed under the control of the upstream promoter and the downstream untranslated region of the Neurog1 gene. Cre activity was detected in cochlear ganglion neurons of transgenic mice when crossed with a cre-reporter strain and dosing with tamoxifen. |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes are not viable.
When using the B6;129P-Tg(Neurog1-cre/ERT2)1Good/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008529 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Neurog1-cre/ESR1)1Good |
Frozen Mouse Embryo | B6;129P-Tg(Neurog1-cre/ERT2)1Good/J | $2595.00 |
Frozen Mouse Embryo | B6;129P-Tg(Neurog1-cre/ERT2)1Good/J | $2595.00 |
Frozen Mouse Embryo | B6;129P-Tg(Neurog1-cre/ERT2)1Good/J | $3373.50 |
Frozen Mouse Embryo | B6;129P-Tg(Neurog1-cre/ERT2)1Good/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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