129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ

Stock number: 008523
Product name: 129S6-podocin-cre
Research areas: Research Tools

Description

These 129S6 Podocin-Cre mice (harboring the p2.5P-Cre transgene) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.

Detailed description

Mice harboring the p2.5P-Cre transgene are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These 2.5P-Cre transgenic mice may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.

Development

The p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. Reports indicate that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Mice from founder line 295 were found to have podocyte-specific cre activity, were backcrossed to C57BL/6J (Stock No. 008205) prior to backcross to 129S6/SvEvTac for 7 generations. The T1DR received the 129S6-2.5P-Cre transgenic at generation N5 and used speed congenic technology to complete the 129S6 congenic backcross. SNP data indicates that no C57BL/6 or SJL contaminating genome outside of the congenic region and the data indicate that the transgene inserted into chromosome 4.

Allele

Symbol: Tg(NPHS2-cre)295Lbh
Name: transgene insertion 295, Lawrence B Holzman
MGI accession ID: MGI:2450359
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(NPHS2-cre)295Lbh
Marker name: transgene insertion 295, Lawrence B Holzman
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase expression in podocytes within kidney glomeruli.
Molecular note: The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase expression in podocytes.
General note: 24 transgenic founders were created. Phenotypic analysis was performed on founder line #295.