These 129S6 Podocin-Cre mice (harboring the p2.5P-Cre transgene) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.
Frank Brosius, University of Michigan Medical School De
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Mice harboring the p2.5P-Cre transgene are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These 2.5P-Cre transgenic mice may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.
The p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. Reports indicate that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Mice from founder line 295 were found to have podocyte-specific cre activity, were backcrossed to C57BL/6J (Stock No. 008205) prior to backcross to 129S6/SvEvTac for 7 generations. The T1DR received the 129S6-2.5P-Cre transgenic at generation N5 and used speed congenic technology to complete the 129S6 congenic backcross. SNP data indicates that no C57BL/6 or SJL contaminating genome outside of the congenic region and the data indicate that the transgene inserted into chromosome 4.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | cre recombinase expression in podocytes within kidney glomeruli. |
Allele Name | transgene insertion 295, Lawrence B Holzman |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | 2.5P-Cre; NPHS2.Cre; pod-Cre; podocin-Cre; PodoCre; Tg(NPHS2-cre)1Lbh |
Gene Symbol and Name | Tg(NPHS2-cre)295Lbh, transgene insertion 295, Lawrence B Holzman |
Gene Synonym(s) | |
Promoter | NPHS2, NPHS2, podocin, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre recombinase expression in podocytes within kidney glomeruli. |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
General Note | 24 transgenic founders were created. Phenotypic analysis was performed on founder line #295. |
Molecular Note | The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase expression in podocytes. |
Mutations Made By | Lawrence Holzman, University of Pennsylvania |
When maintaining a live colony, hemizygous mice may be bred with wildtype siblings. The donating investigator reports that homozygous mice may be bred together.
When using the 129S6-podocin-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008523 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(NPHS2-cre)295Lbh |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.