Floxed homozygous mice express intact nicastrin gene. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These mice are useful to study the role of g-secretase in various tissues in combination with tissue specific cre lines.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ncstn | nicastrin |
Floxed homozygous mice express intact nicastrin gene. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). General deletion in all tissues results in E9.0 embryonic lethality. g-secretase activity is abolished in cells derived from homozygous cre-recombined mice. These mice are useful to study the role of g-secretase in various tissues in combination with tissue specific cre lines.
A neomycin resistance cassette, flanked by loxP and Frt sites was introduced to intron 2 and an additional loxP site was placed in intron 3 using (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 3 or more generations by the donating laboratory.
Allele Name | targeted mutation 1, Thomas C Sudhof |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Ncstn, nicastrin |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 1 |
Molecular Note | A neomycin resistance cassette, flanked by loxP and Frt sites was introduced to intron 2 and an additional loxP site was placed in intron 3. Floxed homozygous mice express intact nicastrin gene. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When using the NCF mouse strain in a publication, please cite the originating article(s) and include JAX stock #008476 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ncstn<tm1Sud> |
Frozen Mouse Embryo | B6;129-Ncstn<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Ncstn<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Ncstn<tm1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Ncstn<tm1Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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