B6;129-Ncstn^tm1Sud/J

Stock number: 008476
Product name: NCF
Research areas: Neurobiology Research, Research Tools

Description

Floxed homozygous mice express intact nicastrin gene. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These mice are useful to study the role of g-secretase in various tissues in combination with tissue specific cre lines.

Detailed description

Floxed homozygous mice express intact nicastrin gene. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). General deletion in all tissues results in E9.0 embryonic lethality. g-secretase activity is abolished in cells derived from homozygous cre-recombined mice. These mice are useful to study the role of g-secretase in various tissues in combination with tissue specific cre lines.

Development

A neomycin resistance cassette, flanked by loxP and Frt sites was introduced to intron 2 and an additional loxP site was placed in intron 3 using (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 3 or more generations by the donating laboratory.

Allele

Symbol: Ncstn^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:4943563
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ncstn
Marker name: nicastrin
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin resistance cassette, flanked by loxP and Frt sites was introduced to intron 2 and an additional loxP site was placed in intron 3. Floxed homozygous mice express intact nicastrin gene.