The miR-106b~25 (microRNA106b-25) cluster, located in intron 13 of the Mcm (minichromosome maintenance deficient 7 (S. cerevisiae)) gene, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-106b~25delta) are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain may be useful in characterizing this genetic region, a paralog of the miR-17~92 region.
Dr. Tyler Jacks, Massachusetts Institute of Technology
The miR-106b~25 (Mir106b, Mir93, Mir25) cluster, located in intron 13 of the Mcm7 (minichromosome maintenance deficient 7 (S. cerevisiae)) gene, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-106b~25delta) are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain may be useful in characterizing this genetic region, a paralog of the miR-17~92 region. Expression of Mcm7 is unaffected.
The Mirn106b, Mirn93, and Mirn25 genes were replaced by an Frt-flanked neomycin cassette. The targeting vector was introduced to V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells. The neomycin cassette was removed through Flp-mediated excision via a C57BL/6 background strain. This strain was maintained on a mixed C57BL/6 and 129S4 background by the donating laboratory.
|Allele Name||targeted mutation 1.1, Tyler Jacks|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mirc3, microRNA cluster 3, including Mir25 through Mir106b|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||The Frt-flanked neomycin cassette replacing the microRNA cluster was removed through Flp-mediated excision.|
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the STOCK Mirc3tm1.1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008460 in your Materials and Methods section.
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