STOCK Mirc3^tm1.1Tyj/J

Stock number: 008460

Description

The miR-106b~25 (microRNA106b-25) cluster, located in intron 13 of the Mcm (minichromosome maintenance deficient 7 (S. cerevisiae)) gene, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-106b~25^delta) are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain may be useful in characterizing this genetic region, a paralog of the miR-17~92 region.

Detailed description

The miR-106b~25 (Mir106b, Mir93, Mir25) cluster, located in intron 13 of the Mcm7 (minichromosome maintenance deficient 7 (S. cerevisiae)) gene, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-106b~25^delta) are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain may be useful in characterizing this genetic region, a paralog of the miR-17~92 region. Expression of Mcm7 is unaffected.

Development

The Mirn106b, Mirn93, and Mirn25 genes were replaced by an Frt-flanked neomycin cassette. The targeting vector was introduced to V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells. The neomycin cassette was removed through Flp-mediated excision via a C57BL/6 background strain. This strain was maintained on a mixed C57BL/6 and 129S4 background by the donating laboratory.

Allele

Symbol: Mirc3^tm1.1Tyj
Name: targeted mutation 1.1, Tyler Jacks
MGI accession ID: MGI:3790969
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Mirc3
Marker name: microRNA cluster 3, including Mir25 through Mir106b
Chromosome: 5
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Molecular note: The Frt-flanked neomycin cassette replacing the microRNA cluster was removed through Flp-mediated excision.