The miR-106b~25 (microRNA106b-25) cluster, located in intron 13 of the Mcm (minichromosome maintenance deficient 7 (S. cerevisiae)) gene, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-106b~25delta) are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain may be useful in characterizing this genetic region, a paralog of the miR-17~92 region.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Mirc3 | microRNA cluster 3, including Mir25 through Mir106b |
The miR-106b~25 (Mir106b, Mir93, Mir25) cluster, located in intron 13 of the Mcm7 (minichromosome maintenance deficient 7 (S. cerevisiae)) gene, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-106b~25delta) are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain may be useful in characterizing this genetic region, a paralog of the miR-17~92 region. Expression of Mcm7 is unaffected.
The Mirn106b, Mirn93, and Mirn25 genes were replaced by an Frt-flanked neomycin cassette. The targeting vector was introduced to V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells. The neomycin cassette was removed through Flp-mediated excision via a C57BL/6 background strain. This strain was maintained on a mixed C57BL/6 and 129S4 background by the donating laboratory.
Allele Name | targeted mutation 1.1, Tyler Jacks |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | miR-106b~25delta |
Gene Symbol and Name | Mirc3, microRNA cluster 3, including Mir25 through Mir106b |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 5 |
Molecular Note | The Frt-flanked neomycin cassette replacing the microRNA cluster was removed through Flp-mediated excision. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the STOCK Mirc3tm1.1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008460 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mirc3<tm1.1Tyj> |
Frozen Mouse Embryo | STOCK Mirc3<tm1.1Tyj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Mirc3<tm1.1Tyj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Mirc3<tm1.1Tyj>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Mirc3<tm1.1Tyj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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