Overview

Also Known As:RAG2 KO

RAG2 knock-out mice have impaired T and B-cell development and may be useful in the study of hematopoietic and immune system defects, cancer, toxicology, and xenograft/transplant studies.

Donating Investigator

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
	N9+N4F14 (2019-12-06 00:00:00)

Rag2^tm1.1Cgn

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Rag2	recombination activating gene 2

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Developmental Biology Research
Hematological Research
Virology Research
Internal/Organ Research

View All Research Applications

Base Price

Starting at:

$152.00 Domestic price for female 4-week

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Details

Detailed Description

These RAG-2^del mutant mice harbor a pan deletion of exon 3 of the targeted locus. Homozygotes (RAG-2^del/del) are viable and fertile, with pan deletion of the entire RAG-2 protein coding region. Homozygous mice may be expected to have the same knockout phenotype as other RAG-2 null mutants or similarly created RAG-2 exon 3 pan-deleted mutants; with hematopoietic and immune system defects including arrested B cell and T cell development at the pro-B and the pro-T cell stages, respectively. These RAG-2^del mice may be useful in studying the role of RAG-2 in B cell and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to insert a loxP-flanked neomycin resistance gene on one side, and a third loxP site on the other side of exon 3 of the recombination activating gene 2 (Rag2) locus. This construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing vector to remove the selection cassette. ES cells in which the neomycin resistance cassette was deleted (leaving a single loxP site upstream, and a single loxP downstream of exon 3) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to generate RAG-2^fl mice. The RAG-2^fl mice were bred with transgenic mice on mixed genetic background, and then bred with mutant mice on a C57BL/6 genetic background for 8 generations (see SNP note below). The donating investigator reports that both the transgene and other mutant gene were bred out of the RAG-2^fl mice prior to arrival at The Jackson Laboratory (as Stock No. 008309). Upon arrival, some RAG-2^fl males were bred to cre-deleter females (Zp3-Cre transgenic mice on a C57BL/6 genetic background; Stock No. 003651), and then F1 males were bred back to B6.Zp3-Cre females. The resulting RAG-2^del (exon 3 deleted) offspring were bred to C57BL/6J inbred mice (selecting for the RAG-2^del mutation and against the cre transgene) for at least 1 generation to generate this RAG-2 "knockout" strain (Stock No. 008449).

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Hao Z; Rajewsky K. 2001. Homeostasis of peripheral b cells in the absence of b cell influx from the bone marrow. J Exp Med 194(8):1151-64PubMed: 11602643 MGI: J:72214

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Genetics

Rag2^tm1.1Cgn

Allele Symbol: Rag2^tm1.1Cgn

Allele Name	targeted mutation 1.1, University of Cologne
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	RAG-2^del
Gene Symbol and Name	Rag2, recombination activating gene 2
Gene Synonym(s)
Strain of Origin	B6.Cg-Thy1^a
Chromosome	2
Molecular Note	This allele is a derivative of Rag2^tm1Cgn in which the sequences flanked by loxP sites were deleted in vivo by crossing mice carrying this allele to a deleter strain.
Mutations Made By	Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - B and T cell deficiency
  - B cell deficiency
- Immunodeficiency
  - B and T cell deficiency
  - B cell defects
  - B cell deficiency
  - T cell deficiency
- T Cell Receptor Signaling Defects
  - B and T cell deficiency
  - B and T cell deficiency, xenograft/transplant host
- Inflammation
  - B and T cell deficiency
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - hematopoietic development
Research Tools
- Toxicology Research
  - B and T cell deficiency, xenograft transplant host
  - xenograft/transplant host
- Immunology, Inflammation and Autoimmunity Research
  - B and T cell deficiency
  - B cell deficiency
  - T cell deficiency
  - T cell deficiency, xenograft/transplant host
  - T Cell Receptor Deficiency
- Hematological Research
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
  - B cell deficiency
  - T cell deficiency
Developmental Biology Research
- Lymphoid Tissue Defects
  - hematopoietic defects
- Internal/Organ Defects
  - hematopoietic defects
Hematological Research
- Hematopoietic Defects
- Immunological Defects
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - T cell deficiency
- Thymus Defects
  - B and T cell deficient

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Rag2^tm1.1Cgn/Rag2^tm1.1Cgn
C57BL/6-Rag2

hematopoietic system phenotype

arrested B cell differentiation
- B cell development is arrested at the pro-B cell stage

arrested T cell differentiation
- T cell development is arrested at the pro-T cell stage

immune system phenotype

arrested B cell differentiation
- B cell development is arrested at the pro-B cell stage

arrested T cell differentiation
- T cell development is arrested at the pro-T cell stage

References

Hao Z; Rajewsky K. 2001. Homeostasis of peripheral b cells in the absence of b cell influx from the bone marrow. J Exp Med 194(8):1151-64PubMed: 11602643 MGI: J:72214

Additional - Rag2^tm1.1Cgn related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Rag2 Alternate 1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous RAG2 KO mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the RAG2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #008449 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available Now

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Rag2<tm1.1Cgn>

Related Products and Services

Frozen Mouse Embryo	B6(Cg)-Rag2<tm1.1Cgn>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6(Cg)-Rag2<tm1.1Cgn>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6(Cg)-Rag2<tm1.1Cgn>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6(Cg)-Rag2<tm1.1Cgn>/J Frozen Embryo	$3373.50

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Email: TechTran@jax.org

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Also Known As:RAG2 KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Rag2tm1.1Cgn

Allele Symbol: Rag2tm1.1Cgn

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Rag2tm1.1Cgn/Rag2tm1.1CgnC57BL/6-Rag2

hematopoietic system phenotype

immune system phenotype

References

Additional - Rag2tm1.1Cgn related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Rag2^tm1.1Cgn

Allele Symbol: Rag2^tm1.1Cgn

Genotype: Rag2^tm1.1Cgn/Rag2^tm1.1Cgn
C57BL/6-Rag2

Additional - Rag2^tm1.1Cgn related