Mice homozygous for both Sele (selectin, endothelial cell) and Selp (selectin, platelet) mutations are viable and fertile. They are characterized by leukocytosis, spontaneous bacterial infections, dermatitis and defective leukocyte recruitment in many models of inflammation.
Dr. Richard Hynes, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Selp | selectin, platelet |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sele | selectin, endothelial cell |
Mice homozygous for both the Seletm1Hyn Selptm1Hyn mutations are viable and fertile. They are characterized by leukocytosis, spontaneous bacterial infections, dermatitis and defective leukocyte recruitment in many models of inflammation.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The Seletm1Hyn mutation was created by replacing the exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain with a PGK-hygro cassette. To create the Selptm1Hyn mutation, a portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was deleted by the insertion of PGK-neo cassette. The two mutations were introduced and selected sequentially in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This line has been backcrossed from a mixed C57BL/6 and 129P2 background to C57BL/6 for more than 10 generations.
Allele Name | targeted mutation 1, Richard Hynes |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | P-; P-selectin-; P-Selp- |
Gene Symbol and Name | Selp, selectin, platelet |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was replaced with a PGK-neo cassette. Mice were treated with lipopolysaccharide to augment the normally low levels of selectin in lung and liver tissue. A longer transcript, resulting from aberrant or cryptic splicing involving the neomycin cassette, was detected at low levels in mutant mice by Northern blot analysis. RT-PCR further confirmed the presence of low levels of transcript in homozygous mice. Immunofluorescence analysis of activated platelets and lung sections from homozygous mutant mice showed an absence of encoded protein. These results were further confirmed by flow cytometric analysis of activated platelets and metabolic labeling of lung tissue. |
Mutations Made By | Dr. Richard Hynes, Massachusetts Institute of Technology |
Allele Name | targeted mutation 1, Richard Hynes |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | E-selectin-; Sele- |
Gene Symbol and Name | Sele, selectin, endothelial cell |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | A modified D3 ES cell line heterozygous for the Selptm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal message for both genes in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in cis with the Selptm1Hyn mutation. Due to their genomic proximity, the two null alleles should propagate together through the offspring. |
Mutations Made By | Dr. Richard Hynes, Massachusetts Institute of Technology |
When maintained as a live colony, homozygotes may be bred.
When using the B6.129S2-Seletm1Hyn Selptm1Hyn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008437 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Sele<tm1Hyn> Heterozygous for Selp<tm1Hyn> |
Frozen Mouse Embryo | B6.129S2-Sele<tm1Hyn> Selp<tm1Hyn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Sele<tm1Hyn> Selp<tm1Hyn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Sele<tm1Hyn> Selp<tm1Hyn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Sele<tm1Hyn> Selp<tm1Hyn>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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