C3FeLe.B6-a Trl/J

Stock number: 008425
Research areas: Neurobiology Research, Reproductive Biology Research

Description

Mice heterozygous for the Trl, trembler-like mutation have an abnormal gait and tremors by approximately three weeks of age. Males are poor breeders.
This strain is extinct, but DNA is available.

Detailed description

Mice heterozygous for the trembler-like mutation have an abnormal gait and tremors by approximately three weeks of age. Males are poor breeders.

Development

The trembler-like mutation arose spontaneously on a transgenic subline of C57BL/6J at The Jackson Laboratory in 2006. The mutation bearing subline was bred to C3FeLe.B6-a/J to improve the viability of the line and has been maintained by backcrossing a female heterozygote to a C3FeLe.B6-a/J male. In 2008 this strain reached generation N5.

Allele

Symbol: Trl
Name: trembler-like
MGI accession ID: MGI:3814701
Generation method: Spontaneous
Marker symbol: Trl
Marker name: trembler-like
Chromosome: UN
Strain of origin: C57BL/6J-Tg(RP24-226L7)28Dn
Molecular note: Mice carrying this new spontaneous mutation were discovered in a research colony of C57BL/6J-Tg(RP24-226L7)28Dn mice at the Jackson Laboratory.

Allele

Symbol: a
Name: nonagouti
MGI accession ID: MGI:1855937
Generation method: Spontaneous
Marker symbol: a
Marker name: nonagouti
Marker synonyms: agouti; agouti signal protein; AGSW; AGTI; AGTIL; As; ASP; SHEP9
Chromosome: 2
Strain of origin: old mutant of the mouse fancy
Molecular note: Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.
General note: Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039