Mice heterozygous for the Trl, trembler-like mutation have an abnormal gait and tremors by approximately three weeks of age. Males are poor breeders.
Mice heterozygous for the trembler-like mutation have an abnormal gait and tremors by approximately three weeks of age. Males are poor breeders.
The trembler-like mutation arose spontaneously on a transgenic subline of C57BL/6J at The Jackson Laboratory in 2006. The mutation bearing subline was bred to C3FeLe.B6-a/J to improve the viability of the line and has been maintained by backcrossing a female heterozygote to a C3FeLe.B6-a/J male. In 2008 this strain reached generation N5.
|Gene Symbol and Name||Trl, trembler-like|
|Strain of Origin||C57BL/6J-Tg(RP24-226L7)28Dn|
|Molecular Note||Mice carrying this new spontaneous mutation were discovered in a research colony of C57BL/6J-Tg(RP24-226L7)28Dn mice at the Jackson Laboratory.|
|Gene Symbol and Name||a, nonagouti|
|Gene Synonym(s)||AGSW; AGTI; AGTIL; ASP; As; As; SHEP9; agouti; agouti signal protein; agouti suppressor|
|Strain of Origin||old mutant of the mouse fancy|
|General Note||Phenotypic Similarity to Human Syndrome: Metabolic Syndrome in mice homozygous for Apoetm1Unc and heterozygous for Ay and a (J:177084) |
|Molecular Note||Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats. The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.|