B6;129S4-Pkd1l3^tm1Sul/J

Stock number: 008419
Research areas: Sensorineural Research

Description

Mice homozygous for this targeted mutation of the Pkd1l3 (polycystic kidney disease 1 like 3) gene are viable and fertile and do not display any gross physical or behavioral abnormalities. No expression was detected when the mice were examined by in situ hybridization of the taste buds. No phenotype has been observed in taste behavioral tests.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. No expression was detected when the mice were examined by in situ hybridization of the taste buds. No phenotype has been observed in taste behavioral tests.

Development

A 4.1 kb PacI-PacI genomic fragment of the gene including exons 18-22 was replaced with a 2.0 kb neomycin resistance cassette. The cassette was flanked by Frt and loxP sites and expression is driven by the pGKEM7 promoter: Frt-loxP-pGKEM7neo-Frt-loxP. The vector was introduced to (C57BL/6J x 129S4/SvJae)F1-derived embryonic stem (ES) cells. This strain was backcrossed twice to C57BL/6 by the donating laboratory.

Allele

Symbol: Pkd1l3^tm1Sul
Name: targeted mutation 1, Susan Sullivan
MGI accession ID: MGI:3784517
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Pkd1l3
Marker name: polycystic kidney disease 1 like 3
Chromosome: 8
Strain of origin: (C57BL/6J x 129S4/SvJae)F1
Molecular note: A 4.1 kb PacI-PacI genomic fragment of the gene including exons 17-21 was replaced with a 2.0 kb neomycin resistance cassette. The cassette was flanked by Frt and loxP sites and expression is driven by the pGKEM7 promoter: Frt-loxP-pGKEM7neo-Frt-loxP. This eliminates transmembrane domains 2 through 5 and causes a downstream shift in reading frame with consequent early stop codons. The predicted truncated protein lacks the ion channel pore and is therefore non functional. No expression was detected when the mice were examined by in situ hybridization of the taste buds.