Mice homozygous for this targeted mutation of the Pkd1l3 (polycystic kidney disease 1 like 3) gene are viable and fertile and do not display any gross physical or behavioral abnormalities. No expression was detected when the mice were examined by in situ hybridization of the taste buds. No phenotype has been observed in taste behavioral tests.
Susan Sullivan, NIH/NIDCD
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Pkd1l3 | polycystic kidney disease 1 like 3 |
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. No expression was detected when the mice were examined by in situ hybridization of the taste buds. No phenotype has been observed in taste behavioral tests.
A 4.1 kb PacI-PacI genomic fragment of the gene including exons 18-22 was replaced with a 2.0 kb neomycin resistance cassette. The cassette was flanked by Frt and loxP sites and expression is driven by the pGKEM7 promoter: Frt-loxP-pGKEM7neo-Frt-loxP. The vector was introduced to (C57BL/6J x 129S4/SvJae)F1-derived embryonic stem (ES) cells. This strain was backcrossed twice to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 1, Susan Sullivan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PKD1L3 KO |
Gene Symbol and Name | Pkd1l3, polycystic kidney disease 1 like 3 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6J x 129S4/SvJae)F1 |
Chromosome | 8 |
Molecular Note | A 4.1 kb PacI-PacI genomic fragment of the gene including exons 17-21 was replaced with a 2.0 kb neomycin resistance cassette. The cassette was flanked by Frt and loxP sites and expression is driven by the pGKEM7 promoter: Frt-loxP-pGKEM7neo-Frt-loxP. This eliminates transmembrane domains 2 through 5 and causes a downstream shift in reading frame with consequent early stop codons. The predicted truncated protein lacks the ion channel pore and is therefore non functional. No expression was detected when the mice were examined by in situ hybridization of the taste buds. |
Mutations Made By | Susan Sullivan, NIH/NIDCD |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129S4-Pkd1l3tm1Sul/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008419 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Pkd1l3<tm1Sul> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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