These transgenic mice express the human interleukin 6 (IL6) gene under the direction of the mouse histocompatibility 2, D region (H-2LD) promoter. Homozygotes begin to develop up to 10-fold enlarged lymph nodes (due to plasma cell accumulation), splenomegaly, glomerulonephritis, and plasma cell tumors as early as 3 months of age.
Siegfried Janz, University of Iowa
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express the human interleukin 6 (IL6) gene under the direction of the mouse histocompatibility 2, D region (H-2LD) promoter. The transgenic insert is located on Chromosome 9. Human IL6 protein is detected in serum by ELISA and mRNA is detected in the spleen, lymph nodes, thymus, heart, lung, liver, kidney, and intestine, but not in brain tissue. As early as three to four months of age, transgenic mice develop up to 10-fold enlarged lymph nodes (due to plasma cell accumulation), plasma cell tumors, splenomegaly, and glomerulonephritis. Although homozygotes for this transgenic insert are viable and fertile, due to the early onset of plasma cell tumors, this strain is maintained as a hemizygote.
A transgenic construct containing the human IL6 gene and rabbit beta-globin sequence under the control of the mouse H-2LD promoter was injected into fertilized C57BL/6 mouse eggs. Founder animals were bred to BALB/cAnPt for more than 20 generations.
Expressed Gene | IL6, interleukin 6, human |
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Site of Expression |
Allele Name | transgene insertion 1, Tadamitsu Kishimoto |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | H-2Ld-hIL6 tg; IL-6 tgm; Ld-IL-6 |
Gene Symbol and Name | Tg(H2-L-IL6)1Kish, transgene insertion 1, Tadamitsu Kishimoto |
Gene Synonym(s) | |
Promoter | H2-L, histocompatibility 2, D region locus L, mouse, laboratory |
Expressed Gene | IL6, interleukin 6, human |
Strain of Origin | C57BL/6 |
Chromosome | 9 |
General Note | Phenotypic Similarity to Human Syndrome: Multicentric Castleman Disease J:128016 |
Molecular Note | A transgenic construct containing the human IL6 gene and rabbit beta-globin sequence under the control of the mouse H-2Ld promoter was injected into fertilized C57BL/6 mouse eggs. Human IL6 protein is detected in serum by ELISA. mRNA is detected in the spleen, lymph nodes, thymus, heart, lung, liver, kidney, and intestine, but not in brain tissue. |
Mutations Made By | Siegfried Janz, University of Iowa |
When maintaining a live colony, these mice are bred as hemizygotes. Homozygotes begin to develop plasma cell tumors as early as 3 months of age.
When using the C.B6-Tg(H2-L-IL6)1Kish/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008372 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(H2-L-IL6)1Kish |
Frozen Mouse Embryo | C.B6-Tg(H2-L-IL6)1Kish/J | $2595.00 |
Frozen Mouse Embryo | C.B6-Tg(H2-L-IL6)1Kish/J | $2595.00 |
Frozen Mouse Embryo | C.B6-Tg(H2-L-IL6)1Kish/J | $3373.50 |
Frozen Mouse Embryo | C.B6-Tg(H2-L-IL6)1Kish/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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