These transgenic mice express the human interleukin 6 (IL6) gene under the direction of the mouse histocompatibility 2, D region ( H-2LD) promoter. Homozygotes begin to develop up to 10-fold enlarged lymph nodes (due to plasma cell accumulation), splenomegaly, glomerulonephritis, and plasma cell tumors as early as 3 months of age.
Siegfried Janz, University of Iowa
These transgenic mice express the human interleukin 6 (IL6) gene under the direction of the mouse histocompatibility 2, D region ( H-2LD) promoter. The transgenic insert is located on Chromosome 9. Human IL6 protein is detected in serum by ELISA and mRNA is detected in the spleen, lymph nodes, thymus, heart, lung, liver, kidney, and intestine, but not in brain tissue. As early as three to four months of age, transgenic mice develop up to 10-fold enlarged lymph nodes (due to plasma cell accumulation), plasma cell tumors, splenomegaly, and glomerulonephritis. Although homozygotes for this transgenic insert are viable and fertile, due to the early onset of plasma cell tumors, this strain is maintained as a hemizygote.
A transgenic construct containing the human IL6 gene and rabbit beta-globin sequence under the control of the mouse H-2LD promoter was injected into fertilized C57BL/6 mouse eggs. Founder animals were bred to BALB/cAnPt for more than 20 generations.
|Expressed Gene||IL6, interleukin 6, human|
|Site of Expression|
|Allele Name||transgene insertion 1, Tadamitsu Kishimoto|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||transgene insertion 1, Tadamitsu Kishimoto; Tg(H2-L-IL6)1Kish|
|Gene Symbol and Name||Tg(H2-L-IL6)1Kish, transgene insertion 1, Tadamitsu Kishimoto|
|Promoter||H2-L, histocompatibility 2, D region locus L, mouse, laboratory|
|Expressed Gene||IL6, interleukin 6, human|
|Strain of Origin||C57BL/6|
|General Note||Phenotypic Similarity to Human Syndrome: Multicentric Castleman Disease J:128016|
|Molecular Note||A transgenic construct containing the human IL6 gene and rabbit beta-globin sequence under the control of the mouse H-2Ld promoter was injected into fertilized C57BL/6 mouse eggs. Human IL6 protein is detected in serum by ELISA. mRNA is detected in the spleen, lymph nodes, thymus, heart, lung, liver, kidney, and intestine, but not in brain tissue.|
|Mutations Made By|| |
Siegfried Janz, University of Iowa
When maintaining a live colony, these mice are bred as hemizygotes. Homozygotes begin to develop plasma cell tumors as early as 3 months of age.
When using the C.B6-Tg(H2-L-IL6)1Kish/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008372 in your Materials and Methods section.
|Hemizygous or Non carrier for Tg(H2-L-IL6)1Kish|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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