Overview

Also Known As: C.H2-Ld-hu-IL6 (Ki/Pt), C.H2-Ld-hu-IL-6

These transgenic mice express the human interleukin 6 (IL6) gene under the direction of the mouse histocompatibility 2, D region ( H-2L^D) promoter. Homozygotes begin to develop up to 10-fold enlarged lymph nodes (due to plasma cell accumulation), splenomegaly, glomerulonephritis, and plasma cell tumors as early as 3 months of age.

Donating Investigator

Siegfried Janz, University of Iowa

Genetic overview

Genetic Background	Generation

Tg(H2-L-IL6)1Kish

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

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Research Applications

Cancer Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These transgenic mice express the human interleukin 6 (IL6) gene under the direction of the mouse histocompatibility 2, D region ( H-2L^D) promoter. The transgenic insert is located on Chromosome 9. Human IL6 protein is detected in serum by ELISA and mRNA is detected in the spleen, lymph nodes, thymus, heart, lung, liver, kidney, and intestine, but not in brain tissue. As early as three to four months of age, transgenic mice develop up to 10-fold enlarged lymph nodes (due to plasma cell accumulation), plasma cell tumors, splenomegaly, and glomerulonephritis. Although homozygotes for this transgenic insert are viable and fertile, due to the early onset of plasma cell tumors, this strain is maintained as a hemizygote.

Development

A transgenic construct containing the human IL6 gene and rabbit beta-globin sequence under the control of the mouse H-2L^D promoter was injected into fertilized C57BL/6 mouse eggs. Founder animals were bred to BALB/cAnPt for more than 20 generations.

Expression Data

Expressed Gene	IL6, interleukin 6, human
Site of Expression

Control Suggestions

Noncarrier

Approximate Controls

001026 BALB/cByJ

Additional Information

Considerations for Choosing Controls

Selected References

Suematsu S; Matsusaka T; Matsuda T; Ohno S; Miyazaki J; Yamamura K; Hirano T; Kishimoto T. 1992. Generation of plasmacytomas with the chromosomal translocation t(12;15) in interleukin 6 transgenic mice. Proc Natl Acad Sci U S A 89(1):232-5PubMed: 1729694 MGI: J:40405

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Genetics

Tg(H2-L-IL6)1Kish

Allele Symbol: Tg(H2-L-IL6)1Kish

Allele Name	transgene insertion 1, Tadamitsu Kishimoto
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	transgene insertion 1, Tadamitsu Kishimoto; Tg(H2-L-IL6)1Kish
Gene Symbol and Name	Tg(H2-L-IL6)1Kish, transgene insertion 1, Tadamitsu Kishimoto
Gene Synonym(s)	L^d-IL-6
Promoter	H2-L, histocompatibility 2, D region locus L, mouse, laboratory
Expressed Gene	IL6, interleukin 6, human
Strain of Origin	C57BL/6
Chromosome	9
General Note	Phenotypic Similarity to Human Syndrome: Multicentric Castleman Disease J:128016
Molecular Note	A transgenic construct containing the human IL6 gene and rabbit beta-globin sequence under the control of the mouse H-2L^d promoter was injected into fertilized C57BL/6 mouse eggs. Human IL6 protein is detected in serum by ELISA. mRNA is detected in the spleen, lymph nodes, thymus, heart, lung, liver, kidney, and intestine, but not in brain tissue.
Mutations Made By	Siegfried Janz, University of Iowa

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cancer Research
- Increased Tumor Incidence

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(H2-L-IL6)1Kish/Tg(H2-L-IL6)1Kish
C57BL/6-Tg(H2-L-IL6)1Kish

hematopoietic system phenotype

abnormal bone marrow cell morphology/development
- bone marrow shows an increase in Gr-1 positive cells
- erythrobast islets are severely decreased in the bone marrow
- increase in neutrophils and mild increase in immature myeloid cells and megakaryocytes in the bone marrow

abnormal definitive hematopoiesis
- Normal - treatment with an anti-IL6R antibody, MR16-1, prevents the increase in serum IgG1 levels and the development of splenomegaly and suppresses hematological abnormalities, blood chemistry abnormalities and glomerulonephritis

abnormal myelopoiesis
- mild increase in immature myeloid cells in the bone marrow

anemia
- anemia becomes severe with age

decreased erythrocyte cell number

decreased hemoglobin content

enlarged spleen
- by 3-4 months of age

increased IgG1 level
- more than 100-fold increase in IgG1 levels with aging

increased megakaryocyte cell number
- mild increase in megakaryocytes in the bone marrow

increased monocyte cell number
- increase in peripheral blood monocytes

increased neutrophil cell number
- increase in neutrophils in the bone marrow
- increase in peripheral blood neutrophils

increased plasma cell number
- large number of plasma cells in the spleen and lymph nodes
- plasma cell infiltration in liver and kidney

thrombocytosis
- platelet counts are increased by 10 weeks of age but then subsequently undergo a decrease

homeostasis/metabolism phenotype

decreased circulating alkaline phosphatase level

decreased circulating glucose level

decreased circulating serum albumin level

decreased circulating triglyceride level

increased circulating total protein level

increased urine protein level
- 71.4% of mice develop proteinuria at 18 weeks of age

immune system phenotype

abnormal myelopoiesis
- mild increase in immature myeloid cells in the bone marrow

enlarged lymph nodes
- by 3-4 months of age, mice show a 10-fold increase in lymph node size due to accumulation of plasma cells

enlarged spleen
- by 3-4 months of age

glomerulonephritis
- by 3-4 months of age
- some mice develop mesangial proliferative glomerulonephritis

increased IgG1 level
- more than 100-fold increase in IgG1 levels with aging

increased monocyte cell number
- increase in peripheral blood monocytes

increased neutrophil cell number
- increase in neutrophils in the bone marrow
- increase in peripheral blood neutrophils

increased plasma cell number
- large number of plasma cells in the spleen and lymph nodes
- plasma cell infiltration in liver and kidney

neoplasm

increased plasmacytoma incidence

renal/urinary system phenotype

glomerulonephritis
- by 3-4 months of age
- some mice develop mesangial proliferative glomerulonephritis

increased urine protein level
- 71.4% of mice develop proteinuria at 18 weeks of age

skeleton phenotype

abnormal trabecular bone morphology
- suppression of primary spongiosa formation

decreased osteoblast cell number
- disappearance of cuboidal osteoblasts in bone

References

Suematsu S; Matsusaka T; Matsuda T; Ohno S; Miyazaki J; Yamamura K; Hirano T; Kishimoto T. 1992. Generation of plasmacytomas with the chromosomal translocation t(12;15) in interleukin 6 transgenic mice. Proc Natl Acad Sci U S A 89(1):232-5PubMed: 1729694 MGI: J:40405

Additional - Tg(H2-L-IL6)1Kish related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Tg(H2-L-IL6)1Kish
- QPCR:Tg(H2-L-IL6)1Kish
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are bred as hemizygotes. Homozygotes begin to develop plasma cell tumors as early as 3 months of age.
- Additional Breeding and Husbandry Support
Citation
When using the C.B6-Tg(H2-L-IL6)1Kish/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008372 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or Non carrier for Tg(H2-L-IL6)1Kish

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	C.B6-Tg(H2-L-IL6)1Kish/J	$2595.00
Frozen Mouse Embryo	C.B6-Tg(H2-L-IL6)1Kish/J	$2595.00
Frozen Mouse Embryo	C.B6-Tg(H2-L-IL6)1Kish/J	$3373.50
Frozen Mouse Embryo	C.B6-Tg(H2-L-IL6)1Kish/J	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: C.H2-Ld-hu-IL6 (Ki/Pt), C.H2-Ld-hu-IL-6

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tg(H2-L-IL6)1Kish

Allele Symbol: Tg(H2-L-IL6)1Kish

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(H2-L-IL6)1Kish/Tg(H2-L-IL6)1KishC57BL/6-Tg(H2-L-IL6)1Kish

hematopoietic system phenotype

homeostasis/metabolism phenotype

immune system phenotype

neoplasm

renal/urinary system phenotype

skeleton phenotype

References

Additional - Tg(H2-L-IL6)1Kish related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Genotype: Tg(H2-L-IL6)1Kish/Tg(H2-L-IL6)1Kish
C57BL/6-Tg(H2-L-IL6)1Kish