These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the Nefl, neurofilament, light polypeptide gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in large neurons.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible) | Nefl | neurofilament, light polypeptide |
These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in large neurons. Although the donating investigator reports that homozygous males may not be fertile and homozygous females may have reduced fertility, homozygous colonies at The Jackson Laboratory have no reported fertility problems.
An IRES-creER cassette followed by an Frt-PGK-Neo-Frt selectable marker was knocked in to the 3' UTR of the gene using a 129-derived JHMI-ES1 embryonic stem cell line. The PGK-Neo was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
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Site of Expression | projection neurons (after induction) |
Allele Name | targeted mutation 1, Jeremy Nathans |
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Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Nefltm1(cre/Esr1)Nat; NFL-IRES-creER; NFL-IRES-creER(T) |
Gene Symbol and Name | Nefl, neurofilament, light polypeptide |
Gene Synonym(s) | |
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
Site of Expression | projection neurons (after induction) |
Strain of Origin | 129 |
Chromosome | 14 |
General Note | ES cell line = JHMI-ES1. |
Molecular Note | An IRES-creER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in ganglion cells and horizontal cells of the retina, and in a sparse number of neurons in the brain. |
When maintained as a live colony, homozygous mice may be bred. Although the donating investigator reports that homozygous males may not be fertile and homozygous females may have reduced fertility, homozygous colonies at The Jackson Laboratory have no reported fertility problems.
When using the B6;129-Nefltm1(cre/ERT)Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008363 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nefl<tm1(Cre/Esr1)Nat> |
Frozen Mouse Embryo | B6;129-Nefl<tm1(cre/ERT)Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Nefl<tm1(cre/ERT)Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Nefl<tm1(cre/ERT)Nat>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Nefl<tm1(cre/ERT)Nat>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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