JAX Mouse Strain Datasheet - 008352

NOD.129P2(B6)-Ctsl^tm1Cptr/RclJ

Stock No:: 008352 |; NOD.CatL

Cryo Recovery

Overview

Also Known As: NOD.CatL, NOD.Catl

This congenic NOD strain contains a neomycin cassette disruption in exon 3 of cathepsin L (Ctsl^tm1Cptr). NOD Ctsl deficient mice exhibit defective CD4⁺T cell development and express Foxp3 on CD4⁺ T cells at a 2 fold higher frequency than controls. Homozygous mice are insulitis and diabetes resistant. This model provides a tool for detailed studies to identify the molecular pathways of major lysosomal cysteine proteases, specifically cathepsin L, in immune modulation.

Donating Investigator

Dr. Renee C LeBoeuf, Universtiy of Washington

Genetic overview

Genetic Background	Generation
NOD

Ctsl^tm1Cptr

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ctsl	cathepsin L

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Research Applications

Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research

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Base Price

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$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for this Ctsl targeted mutation are viable, normal in size, and do not display any behavioral abnormalities. Western blot analysis failed to detect any signal in kidneys of homozygous mutant mice. No Ctsl enzymatic activity was detected in the kidney using a synthetic substrate. Donating investigator reports that homozygous females are infertile; while homozygous males breed well. As reported in the B6-Ctsl^tm1Cptr by Roth et al. 2000, the vibrissae of NOD congenic mutant mice have not penetrated the epidermis at birth and the first emergence of fur is delayed by approximately two days. Beginning around three weeks of age NOD mutant mice begin to lose their fur beginning at the head and progressing toward the tail region. Mature mutant mice are always partially devoid of fur. Unlike the observations of Roth et al. there appears to be no distortion of the the expected rates of homozygous NOD congenic mice weaned. Similar to Maehr et al. 2005, no throiditis, sialadenitis, diabetes or insulitis was detected in NOD.Ctsl deficient females aged to 10 months (Hsing et al, 2009). Ctsl deficient mice exhibit defective CD4⁺T cell development leading to a reduced CD4:CD8 ratio. CD4⁺T cells if naive NOD.Ctsl deficient mice exhibit Foxp3 expression at 2 fold higher frequency than wildtype controls (Hsing et al, 2009).

This model provides a tool for detailed studies to identify the molecular pathways of major lysosomal cysteine proteases, specifically cathepsin L in immune modulation.

Development

The Cathepsin L (Ctsl) gene was disrupted via homologous recombination in 129P2/OlaHsd-derived, E14.1 ES cells. A neomycin cassette was inserted into a NcoI site in exon 3 using a SalI linker containing stop codons in all reading frames of the Ctsl gene. The ES cell clone was injected into C57BL/6 blastocysts. The resulting chimeric founder's offspring were bred to C57BL/6J prior to 10 generations of crossing to NOD and intercrossing. Marker assisted analysis indicates 19 known Idd loci are of NOD origin. In 2008, the T1DR received this strain at generation N11F2.

Control Suggestions

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Genetics

Ctsl^tm1Cptr

Allele Symbol: Ctsl^tm1Cptr

Allele Name	targeted mutation 1, Christoph Peters
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	CL-; Cat L^-; L^-; catL-; ctsl-
Gene Symbol and Name	Ctsl, cathepsin L
Gene Synonym(s)	1190035F06Rik; 1190035F06Rik; CATHL; CATL2; CTSL2; CTSU; Cat L; CatL; Ctsl1; MEP; RIKEN cDNA 1190035F06 gene; fs; furless; major excreted protein; nackt; nkt
Strain of Origin	129P2/OlaHsd
Chromosome	13
General Note	In combination with Ctsb^tm1Jde, double homozygous mutant have some similarities but distinct phenotypic characteristics compared to the human syndrome: Neuronal Ceroid Lipofuscinoses (NLCs)
Molecular Note	Insertion of a neomycin resistance cassette into exon 3. Northern and Western analysis failed to detect any signal in homozygous mutant mice. No Ctsl enzymatic activity was detected using a synthetic substrate.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
- Type 1 Diabetes (IDDM)
  - resistant
Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - Type 1 Diabetes

Mammalian Phenotype Terms by Genotype

Genotype: Ctsl^tm1Cptr/Ctsl⁺
NOD.129P2-Ctsl^tm1Cptr

immune system phenotype

decreased susceptibility to autoimmune diabetes
- 55% of female mice exhibit blood glucose levels of greater than 250 mg/dl between 4-6 months of age as compared to 69% of NOD controls
- adoptive transfer of GITR^hi-depleted Treg cells into NOD-Prkdc^scid host results in a diabetes incidence of 41.7%

insulitis
- insulitis scores for diabetic animals are similar to diabetic NOD controls

homeostasis/metabolism phenotype

hyperglycemia
- 55% of female mice exhibit blood glucose levels of greater than 250 mg/dl between 4-6 months of age as compared to 69% of NOD controls

endocrine/exocrine gland phenotype

insulitis
- insulitis scores for diabetic animals are similar to diabetic NOD controls

Genotype: Ctsl^tm1Cptr/Ctsl^tm1Cptr
NOD.129P2-Ctsl^tm1Cptr

immune system phenotype

decreased CD4-positive, alpha beta T cell number
- number of CD4+ T cells is 3-fold reduced in thymus and lymph nodes as compared to NOD control

decreased susceptibility to autoimmune diabetes
- adoptive transfer of GITR^hi-depleted Treg cells into NOD-Prkdc^scid host results in a diabetes incidence of 45.5%
- mice exhibit a complete resistance to diabetes

increased regulatory T cell number
- homozygotes exhibit a 2-fold increase in Foxp3-expressing T cells in comparison to heterozygotes

salivary gland inflammation
- sialadenitis is observed in both diabetic and nondiabetic mice at 6 months of age, however, number of lesions observed is less than number found in NOD mice (6.5 vs. 12-20)

endocrine/exocrine gland phenotype

salivary gland inflammation
- sialadenitis is observed in both diabetic and nondiabetic mice at 6 months of age, however, number of lesions observed is less than number found in NOD mice (6.5 vs. 12-20)

digestive/alimentary phenotype

salivary gland inflammation
- sialadenitis is observed in both diabetic and nondiabetic mice at 6 months of age, however, number of lesions observed is less than number found in NOD mice (6.5 vs. 12-20)

hematopoietic system phenotype

decreased CD4-positive, alpha beta T cell number
- number of CD4+ T cells is 3-fold reduced in thymus and lymph nodes as compared to NOD control

increased regulatory T cell number
- homozygotes exhibit a 2-fold increase in Foxp3-expressing T cells in comparison to heterozygotes

References

Additional - Ctsl^tm1Cptr related

Abboud-Jarrous G; Atzmon R; Peretz T; Palermo C; Gadea BB; Joyce JA; Vlodavsky I. 2008. Cathepsin L is responsible for processing and activation of proheparanase through multiple cleavages of a linker segment. J Biol Chem 283(26):18167-76. PubMed: 18450756 MGI: J:138130
Allan ER; Yates RM. 2015. Redundancy between Cysteine Cathepsins in Murine Experimental Autoimmune Encephalomyelitis. PLoS One 10(6):e0128945. PubMed: 26075905 MGI: J:237812
Benavides F; Perez C; Blando J; Guenet JL; Conti CJ. 2006. The radiation-induced nackt (nkt) allele is a loss-of-function mutation of the mouse cathepsin L gene. J Immunol 176(2):702-3. PubMed: 16393949 MGI: J:108004
Brindle NR; Joyce JA; Rostker F; Lawlor ER; Swigart-Brown L; Evan G; Hanahan D; Shchors K. 2015. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS One 10(4):e0120348. PubMed: 25927437 MGI: J:229244
Dennemarker J; Lohmuller T; Mayerle J; Tacke M; Lerch MM; Coussens LM; Peters C; Reinheckel T. 2010. Deficiency for the cysteine protease cathepsin L promotes tumor progression in mouse epidermis. Oncogene 29(11):1611-21. PubMed: 20023699 MGI: J:160397
Dennemarker J; Lohmuller T; Muller S; Aguilar SV; Tobin DJ; Peters C; Reinheckel T. 2010. Impaired turnover of autophagolysosomes in cathepsin L deficiency. Biol Chem 391(8):913-22. PubMed: 20536383 MGI: J:186898
Duewell P; Kono H; Rayner KJ; Sirois CM; Vladimer G; Bauernfeind FG; Abela GS; Franchi L; Nunez G; Schnurr M; Espevik T; Lien E; Fitzgerald KA; Rock KL; Moore KJ; Wright SD; Hornung V; Latz E. 2010. NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals. Nature 464(7293):1357-61. PubMed: 20428172 MGI: J:159453
Everts V; Korper W; Hoeben KA; Jansen ID; Bromme D; Cleutjens KB; Heeneman S; Peters C; Reinheckel T; Saftig P; Beertsen W. 2006. Osteoclastic bone degradation and the role of different cysteine proteinases and matrix metalloproteinases: differences between calvaria and long bone. J Bone Miner Res 21(9):1399-408. PubMed: 16939398 MGI: J:128089
Felbor U; Kessler B; Mothes W; Goebel HH; Ploegh HL; Bronson RT; Olsen BR. 2002. Neuronal loss and brain atrophy in mice lacking cathepsins B and L. Proc Natl Acad Sci U S A 99(12):7883-8. PubMed: 12048238 MGI: J:77103
Friedrichs B; Tepel C; Reinheckel T; Deussing J; von Figura K; Herzog V; Peters C; Saftig P; Brix K. 2003. Thyroid functions of mouse cathepsins B, K, and L. J Clin Invest 111(11):1733-45. PubMed: 12782676 MGI: J:83877
Funkelstein L; Toneff T; Hwang SR; Reinheckel T; Peters C; Hook V. 2008. Cathepsin L participates in the production of neuropeptide Y in secretory vesicles, demonstrated by protease gene knockout and expression. J Neurochem 106(1):384-91. PubMed: 18410501 MGI: J:137265
Funkelstein L; Toneff T; Mosier C; Hwang SR; Beuschlein F; Lichtenauer UD; Reinheckel T; Peters C; Hook V. 2008. Major role of cathepsin L for producing the peptide hormones ACTH, beta-endorphin, and alpha-MSH, illustrated by protease gene knockout and expression. J Biol Chem 283(51):35652-9. PubMed: 18849346 MGI: J:144593
Gocheva V; Wang HW; Gadea BB; Shree T; Hunter KE; Garfall AL; Berman T; Joyce JA. 2010. IL-4 induces cathepsin protease activity in tumor-associated macrophages to promote cancer growth and invasion. Genes Dev 24(3):241-55. PubMed: 20080943 MGI: J:156936
Gocheva V; Zeng W; Ke D; Klimstra D; Reinheckel T; Peters C; Hanahan D; Joyce JA. 2006. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev 20(5):543-56. PubMed: 16481467 MGI: J:105935
Grimm C; Holdt LM; Chen CC; Hassan S; Muller C; Jors S; Cuny H; Kissing S; Schroder B; Butz E; Northoff B; Castonguay J; Luber CA; Moser M; Spahn S; Lullmann-Rauch R; Fendel C; Klugbauer N; Griesbeck O; Haas A; Mann M; Bracher F; Teupser D; Saftig P; Biel M; Wahl-Schott C. 2014. High susceptibility to fatty liver disease in two-pore channel 2-deficient mice. Nat Commun 5:4699. PubMed: 25144390 MGI: J:225206
Hagemann S; Gunther T; Dennemarker J; Lohmuller T; Bromme D; Schule R; Peters C; Reinheckel T. 2004. The human cysteine protease cathepsin V can compensate for murine cathepsin L in mouse epidermis and hair follicles. Eur J Cell Biol 83(11-12):775-80. PubMed: 15679121 MGI: J:102123
Henningsson F; Yamamoto K; Saftig P; Reinheckel T; Peters C; Knight SD; Pejler G. 2005. A role for cathepsin E in the processing of mast-cell carboxypeptidase A. J Cell Sci 118(Pt 9):2035-42. PubMed: 15860733 MGI: J:98827
Honey K; Benlagha K; Beers C; Forbush K; Teyton L; Kleijmeer MJ; Rudensky AY; Bendelac A. 2002. Thymocyte expression of cathepsin L is essential for NKT cell development. Nat Immunol 3(11):1069-74. PubMed: 12368909 MGI: J:109255
Honey K; Nakagawa T; Peters C; Rudensky A. 2002. Cathepsin L regulates CD4+ T cell selection independently of its effect on invariant chain: a role in the generation of positively selecting peptide ligands. J Exp Med 195(10):1349-58. PubMed: 12021314 MGI: J:112070
Hsing LC; Kirk EA; McMillen TS; Hsiao SH; Caldwell M; Houston B; Rudensky AY; Leboeuf RC. 2009. Roles for cathepsins S, L, and B in insulitis and diabetes in the NOD mouse. J Autoimmun :. PubMed: 19664906 MGI: J:153355
Kalantari P; DeOliveira RB; Chan J; Corbett Y; Rathinam V; Stutz A; Latz E; Gazzinelli RT; Golenbock DT; Fitzgerald KA. 2014. Dual engagement of the NLRP3 and AIM2 inflammasomes by plasmodium-derived hemozoin and DNA during malaria. Cell Rep 6(1):196-210. PubMed: 24388751 MGI: J:206874
Kitamoto S; Sukhova GK; Sun J; Yang M; Libby P; Love V; Duramad P; Sun C; Zhang Y; Yang X; Peters C; Shi GP. 2007. Cathepsin L deficiency reduces diet-induced atherosclerosis in low-density lipoprotein receptor-knockout mice. Circulation 115(15):2065-75. PubMed: 17404153 MGI: J:135371
Koike M; Shibata M; Ohsawa Y; Nakanishi H; Koga T; Kametaka S; Waguri S; Momoi T; Kominami E; Peters C; Figura K; Saftig P; Uchiyama Y. 2003. Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice. Mol Cell Neurosci 22(2):146-61. PubMed: 12676526 MGI: J:82765
Koike M; Shibata M; Waguri S; Yoshimura K; Tanida I; Kominami E; Gotow T; Peters C; von Figura K; Mizushima N; Saftig P; Uchiyama Y. 2005. Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (batten disease). Am J Pathol 167(6):1713-28. PubMed: 16314482 MGI: J:103645
Konjar S; Sutton VR; Hoves S; Repnik U; Yagita H; Reinheckel T; Peters C; Turk V; Turk B; Trapani JA; Kopitar-Jerala N. 2010. Human and mouse perforin are processed in part through cleavage by the lysosomal cysteine proteinase cathepsin L. Immunology 131(2):257-67. PubMed: 20497254 MGI: J:166401
Lennon-Dumenil AM; Roberts RA; Valentijn K; Driessen C; Overkleeft HS; Erickson A; Peters PJ; Bikoff E; Ploegh HL; Wolf Bryant P. 2001. The p41 isoform of invariant chain is a chaperone for cathepsin L. EMBO J 20(15):4055-64. PubMed: 11483509 MGI: J:115604
Maehr R; Hang HC; Mintern JD; Kim YM; Cuvillier A; Nishimura M; Yamada K; Shirahama-Noda K; Hara-Nishimura I; Ploegh HL. 2005. Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice. J Immunol 174(11):7066-74. PubMed: 15905550 MGI: J:99005
Maehr R; Mintern JD; Herman AE; Lennon-Dumenil AM; Mathis D; Benoist C; Ploegh HL. 2005. Cathepsin L is essential for onset of autoimmune diabetes in NOD mice. J Clin Invest 115(10):2934-43. PubMed: 16184198 MGI: J:101527
Miller G; Matthews SP; Reinheckel T; Fleming S; Watts C. 2011. Asparagine endopeptidase is required for normal kidney physiology and homeostasis. FASEB J 25(5):1606-17. PubMed: 21292981 MGI: J:172774
Minokadeh A; Funkelstein L; Toneff T; Hwang SR; Beinfeld M; Reinheckel T; Peters C; Zadina J; Hook V. 2010. Cathepsin L participates in dynorphin production in brain cortex, illustrated by protease gene knockout and expression. Mol Cell Neurosci 43(1):98-107. PubMed: 19837164 MGI: J:158324
Nakagawa T; Roth W; Wong P; Nelson A; Farr A; Deussing J; Villadangos JA; Ploegh H; Peters C; Rudensky AY. 1998. Cathepsin L: critical role in Ii degradation and CD4 T cell selection in the thymus. Science 280(5362):450-3. PubMed: 9545226 MGI: J:82888
Nepal RM; Vesosky B; Turner J; Bryant P. 2008. DM, but not cathepsin L, is required to control an aerosol infection with Mycobacterium tuberculosis. J Leukoc Biol 84(4):1011-8. PubMed: 18591414 MGI: J:140231
Nishimura F; Naruishi H; Naruishi K; Yamada T; Sasaki J; Peters C; Uchiyama Y; Murayama Y. 2002. Cathepsin-L, a key molecule in the pathogenesis of drug-induced and I-cell disease-mediated gingival overgrowth: a study with cathepsin-L-deficient mice. Am J Pathol 161(6):2047-52. PubMed: 12466121 MGI: J:80536
Ohkouchi S; Shibata M; Sasaki M; Koike M; Safig P; Peters C; Nagata S; Uchiyama Y. 2013. Biogenesis and proteolytic processing of lysosomal DNase II. PLoS One 8(3):e59148. PubMed: 23516607 MGI: J:199384
Pensa S; Neoh K; Resemann HK; Kreuzaler PA; Abell K; Clarke NJ; Reinheckel T; Kahn CR; Watson CJ. 2014. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ 21(9):1442-50. PubMed: 24902901 MGI: J:229996
Petermann I; Mayer C; Stypmann J; Biniossek ML; Tobin DJ; Engelen MA; Dandekar T; Grune T; Schild L; Peters C; Reinheckel T. 2006. Lysosomal, cytoskeletal, and metabolic alterations in cardiomyopathy of cathepsin L knockout mice. FASEB J 20(8):1266-8. PubMed: 16636100 MGI: J:111345
Reinheckel T; Hagemann S; Dollwet-Mack S; Martinez E; Lohmuller T; Zlatkovic G; Tobin DJ; Maas-Szabowski N; Peters C. 2005. The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling. J Cell Sci 118(Pt 15):3387-95. PubMed: 16079282 MGI: J:100201
Rocken C; Fandrich M; Stix B; Tannert A; Hortschansky P; Reinheckel T; Saftig P; Kahne T; Menard R; Ancsin JB; Buhling F. 2006. Cathepsin protease activity modulates amyloid load in extracerebral amyloidosis. J Pathol 210(4):478-87. PubMed: 17068745 MGI: J:116135
Roth W; Deussing J; Botchkarev VA; Pauly-Evers M; Saftig P; Hafner A; Schmidt P; Schmahl W; Scherer J; Anton-Lamprecht I; Von Figura K; Paus R; Peters C. 2000. Cathepsin L deficiency as molecular defect of furless: hyperproliferation of keratinocytes and pertubation of hair follicle cycling. FASEB J 14(13):2075-86. PubMed: 11023992 MGI: J:70118
Roy KC; Maricic I; Khurana A; Smith TR; Halder RC; Kumar V. 2008. Involvement of Secretory and Endosomal Compartments in Presentation of an Exogenous Self-Glycolipid to Type II NKT Cells. J Immunol 180(5):2942-50. PubMed: 18292516 MGI: J:131541
Sargeant TJ; Lloyd-Lewis B; Resemann HK; Ramos-Montoya A; Skepper J; Watson CJ. 2014. Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization. Nat Cell Biol 16(11):1057-68. PubMed: 25283994 MGI: J:217705
Sever S; Altintas MM; Nankoe SR; Moller CC; Ko D; Wei C; Henderson J; del Re EC; Hsing L; Erickson A; Cohen CD; Kretzler M; Kerjaschki D; Rudensky A; Nikolic B; Reiser J. 2007. Proteolytic processing of dynamin by cytoplasmic cathepsin L is a mechanism for proteinuric kidney disease. J Clin Invest 117(8):2095-104. PubMed: 17671649 MGI: J:123953
Shen L; Sigal LJ; Boes M; Rock KL. 2004. Important role of cathepsin S in generating peptides for TAP-independent MHC class I crosspresentation in vivo. Immunity 21(2):155-65. PubMed: 15308097 MGI: J:93590
Shimada N; Ohno-Matsui K; Iseki S; Koike M; Uchiyama Y; Wang J; Yoshida T; Sato T; Peters C; Mochizuki M; Morita I. 2010. Cathepsin L in bone marrow-derived cells is required for retinal and choroidal neovascularization. Am J Pathol 176(5):2571-80. PubMed: 20304958 MGI: J:160772
Spira D; Stypmann J; Tobin DJ; Petermann I; Mayer C; Hagemann S; Vasiljeva O; Gunther T; Schule R; Peters C; Reinheckel T. 2007. Cell Type-specific Functions of the Lysosomal Protease Cathepsin L in the Heart. J Biol Chem 282(51):37045-37052. PubMed: 17942402 MGI: J:129020
Steenhuis P; Froemming J; Reinheckel T; Storch S. 2012. Proteolytic cleavage of the disease-related lysosomal membrane glycoprotein CLN7. Biochim Biophys Acta 1822(10):1617-28. PubMed: 22668694 MGI: J:188065
Stypmann J; Glaser K; Roth W; Tobin DJ; Petermann I; Matthias R; Monnig G; Haverkamp W; Breithardt G; Schmahl W; Peters C; Reinheckel T. 2002. Dilated cardiomyopathy in mice deficient for the lysosomal cysteine peptidase cathepsin L. Proc Natl Acad Sci U S A 99(9):6234-9. PubMed: 11972068 MGI: J:76333
Sugita S; Horie S; Nakamura O; Maruyama K; Takase H; Usui Y; Takeuchi M; Ishidoh K; Koike M; Uchiyama Y; Peters C; Yamamoto Y; Mochizuki M. 2009. Acquisition of T regulatory function in cathepsin L-inhibited T cells by eye-derived CTLA-2alpha during inflammatory conditions. J Immunol 183(8):5013-22. PubMed: 19801522 MGI: J:153812
Sun J; Sukhova GK; Zhang J; Chen H; Sjoberg S; Libby P; Xiang M; Wang J; Peters C; Reinheckel T; Shi GP. 2011. Cathepsin L activity is essential to elastase perfusion-induced abdominal aortic aneurysms in mice. Arterioscler Thromb Vasc Biol 31(11):2500-8. PubMed: 21868704 MGI: J:191463
Tholen M; Hillebrand LE; Tholen S; Sedelmeier O; Arnold SJ; Reinheckel T. 2014. Out-of-frame start codons prevent translation of truncated nucleo-cytosolic cathepsin L in vivo. Nat Commun 5:4931. PubMed: 25222295 MGI: J:216506
Tobin DJ; Foitzik K; Reinheckel T; Mecklenburg L; Botchkarev VA; Peters C; Paus R. 2002. The lysosomal protease cathepsin L is an important regulator of keratinocyte and melanocyte differentiation during hair follicle morphogenesis and cycling. Am J Pathol 160(5):1807-21. PubMed: 12000732 MGI: J:109246
Urbich C; Heeschen C; Aicher A; Sasaki K; Bruhl T; Farhadi MR; Vajkoczy P; Hofmann WK; Peters C; Pennacchio LA; Abolmaali ND; Chavakis E; Reinheckel T; Zeiher AM; Dimmeler S. 2005. Cathepsin L is required for endothelial progenitor cell-induced neovascularization. Nat Med 11(2):206-13. PubMed: 15665831 MGI: J:99594
Wilson SR; Peters C; Saftig P; Bromme D. 2009. Cathepsin K activity-dependent regulation of osteoclast actin ring formation and bone resorption. J Biol Chem 284(4):2584-92. PubMed: 19028686 MGI: J:146945
Xu X; Greenland J; Baluk P; Adams A; Bose O; McDonald DM; Caughey GH. 2013. Cathepsin L protects mice from mycoplasmal infection and is essential for airway lymphangiogenesis. Am J Respir Cell Mol Biol 49(3):437-44. PubMed: 23600672 MGI: J:221765
Yang M; Zhang Y; Pan J; Sun J; Liu J; Libby P; Sukhova GK; Doria A; Katunuma N; Peroni OD; Guerre-Millo M; Kahn BB; Clement K; Shi GP. 2007. Cathepsin L activity controls adipogenesis and glucose tolerance. Nat Cell Biol 9(8):970-7. PubMed: 17643114 MGI: J:129484
Zeeuwen PL; van Vlijmen-Willems IM; Cheng T; Rodijk-Olthuis D; Hitomi K; Hara-Nishimura I; John S; Smyth N; Reinheckel T; Hendriks WJ; Schalkwijk J. 2010. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J 24(10):3744-55. PubMed: 20495178 MGI: J:165312

Service	Genotype	Price
	Heterozygous or Wild-type for Cstl<tm1Cptr>

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Also Known As: NOD.CatL, NOD.Catl

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Ctsltm1Cptr

Allele Symbol: Ctsltm1Cptr

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Ctsltm1Cptr/Ctsl+NOD.129P2-Ctsltm1Cptr

immune system phenotype

homeostasis/metabolism phenotype

endocrine/exocrine gland phenotype

Genotype: Ctsltm1Cptr/Ctsltm1CptrNOD.129P2-Ctsltm1Cptr

immune system phenotype

endocrine/exocrine gland phenotype

digestive/alimentary phenotype

hematopoietic system phenotype

References

Additional - Ctsltm1Cptr related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Ctsl^tm1Cptr

Allele Symbol: Ctsl^tm1Cptr

Genotype: Ctsl^tm1Cptr/Ctsl⁺
NOD.129P2-Ctsl^tm1Cptr

Genotype: Ctsl^tm1Cptr/Ctsl^tm1Cptr
NOD.129P2-Ctsl^tm1Cptr

Additional - Ctsl^tm1Cptr related