The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. This strain provides a tool that allows the inducible stable labeling of activated neurons.
Dr. Mark Mayford, The Scripps Research Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Transactivator) |
Allele Type |
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Transgenic (Reporter, Inducible, Transactivator) |
The TetTag mouse is a bi-transgenic mutant that has tetracycline (or a tetracycline analog such as doxycycline) inducible expression of beta-galactosidase in activated neurons. Two independently generated transgenic strains were crossed to produce this bi-transgenic TetTag strain. In the first transgenic construct, the tetracycline-controlled transactivator (tTA) protein and a two hour half-life Green Fluorescent Protein (shEGFP) are expressed under the direction of the fos, FBJ osteosarcoma oncogene, minimal promoter. The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element.
Doxycycline administration prevents expression beta-galactosidase in neurons. In the absence of doxycycline, tau-LacZ is induced in neurons of the dentate gyrus (DG) and CA1 neuron, with most of the LacZ-positive neurons displaying typical morphologies of granule cells and pyramidal neurons in the DG and CA1, respectively. Neurons that were induced to express beta-galactosidase during the absence of doxycycline administration will continue to express beta-galactosidase via the doxycycline insensitive mutant tTAH100Y. Mice hemizygous for each transgene are viable and fertile. The strain is maintained with doxycycline administration to prevent neuronal beta-galactosidase expression. The donating investigator has not attempted to make these mice homozygous for the transgenes. This strain provides a tool that allows the inducible stable labeling of activated neurons.
Of note, cfos-htTA mice (cfos-tTA/cfos-shEGFP) harboring only the co-injected cfos-tTA/cfos-shEGFP transgenes are available at The Jackson Laboratory as Stock No. 018306.
To produce this bi-transgenic line, two independently generated single transgenic lines were crossed.
The first transgenic line was generated by co-injection of a construct containing the tetracycline regulated transactivator (tTA), SV40 polyadenylation signal sequence and the fos, FBJ osteosarcoma oncogene, minimal promoter and a second construct containing a two hour half-life Green Fluorescent Protein under the control of the fos, FBJ osteosarcoma oncogene, minimal promoter, into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregated as a single transgene.
The second transgenic line was generated using a construct including sequence encoding tau-LacZ and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. This construct was injected into fertilized B6D2F2 mouse eggs.
The two independent transgenic lines were then crossed to produce this bi-transgenic strain. The mice were then backcrossed to C57BL/6J for 3 generations.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | activated neurons in the basolateral amygdala |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | activated neurons in the basolateral amygdala |
Allele Name | transgene insertion 1, Mark Mayford |
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Allele Type | Transgenic (Reporter, Transactivator) |
Allele Synonym(s) | cfos-htTA mice |
Gene Symbol and Name | Tg(Fos-tTA,Fos-EGFP*)1Mmay, transgene insertion 1, Mark Mayford |
Gene Synonym(s) | |
Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | activated neurons in the basolateral amygdala |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | A construct containing the Fos (FBJ osteosarcoma oncogene, minimal promoter) driving the tetracycline regulated transactivator (tTA) sequence, the SV40 polyadenylation signal sequence and a second construct containing a two hour half-life Green Fluorescent Protein (shEGFP) under the control of the Fos minimal promoter, were co-injected into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregate as a single transgene. |
Allele Name | transgene insertion 1, Mark Mayford |
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Allele Type | Transgenic (Reporter, Inducible, Transactivator) |
Allele Synonym(s) | tetO-tTA*-tauLacZ |
Gene Symbol and Name | Tg(tetO-lacZ,tTA*)1Mmay, transgene insertion 1, Mark Mayford |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | activated neurons in the basolateral amygdala |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | A construct including the sequence encoding tau-LacZ and a doxycycline-insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of a bi-cistronic tetO promoter (tetracycline-responsive regulatory element) was injected into fertilized eggs. |
When maintaining a live colony, these mice are bred hemizygous for each transgene. The strain is maintained with doxycycline administration to prevent neuronal beta-galactosidase expression. The donating investigator notes that his lab has not been successful in their attempts to generate homozygotes from this line.
When using the TetTag mouse strain in a publication, please cite the originating article(s) and include JAX stock #008344 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Fos-tTA,Fos-EGFP*)1Mmay,Heterozygous or Wild-type for Tg(tetO-lacZ,tTA*)1Mmay |
Frozen Mouse Embryo | B6;DBA-Tg(Fos-tTA Fos-EGFP*)1Mmay Tg(tetO-lacZ tTA*)1Mmay/J | $2595.00 |
Frozen Mouse Embryo | B6;DBA-Tg(Fos-tTA Fos-EGFP*)1Mmay Tg(tetO-lacZ tTA*)1Mmay/J | $2595.00 |
Frozen Mouse Embryo | B6;DBA-Tg(Fos-tTA Fos-EGFP*)1Mmay Tg(tetO-lacZ tTA*)1Mmay/J | $3373.50 |
Frozen Mouse Embryo | B6;DBA-Tg(Fos-tTA Fos-EGFP*)1Mmay Tg(tetO-lacZ tTA*)1Mmay/J | $3373.50 |
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