To produce this bi-transgenic line, two independently generated single transgenic lines were crossed.
The first transgenic line was generated by co-injection of a construct containing the tetracycline regulated transactivator (tTA), SV40 polyadenylation signal sequence and the fos, FBJ osteosarcoma oncogene, minimal promoter and a second construct containing a two hour half-life Green Fluorescent Protein under the control of the fos, FBJ osteosarcoma oncogene, minimal promoter, into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregated as a single transgene.
The second transgenic line was generated using a construct including sequence encoding tau-LacZ and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. This construct was injected into fertilized B6D2F2 mouse eggs.
The two independent transgenic lines were then crossed to produce this bi-transgenic strain. The mice were then backcrossed to C57BL/6J for 3 generations.
