These mutant mice carry a His6-tagged mouse myelocytomatosis oncogene (Myc) cDNA sequence inserted in the endogenous immunoglobulin heavy-chain 2 C-alpha locus (Igh-2), which mimics the human Burkitt lymphoma t(8;14)(q24;q32) translocation and the mouse plasmacytoma-typical T(12;15) translocation. This mutant mouse strain may be useful in studies of Burkitt Lymphoma and plasma cell and B-cell neoplasia.
Siegfried Janz, University of Iowa
These mutant mice carry a His6-tagged mouse Myc gene (cDNA) sequence inserted in the endogenous immunoglobulin heavy-chain 2 C-alpha locus (Igh-2). This insertion mimics the human Burkitt lymphoma t(8;14)(q24;q32) translocation and its counterpart in mice, the plasmacytoma-typical T(12;15) translocation. The His6-tagged Myc is overexpressed in B-cells throughout B-cell development. Mutant mice develop increased numbers of B220hiCD138+ plasmablasts in the bone marrow and display increased B-cell apoptosis and proliferation. 9.3% of mutant mice develop B-cell lymphomas with a mean onset of 11 months. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of B cell and plasma cell neoplasia.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing the cDNA sequence (lacking introns) and the first non-coding exon (with the P1/P2 promoter) of the mouse Myc gene, 1.5kb of Myc 5' flanking sequence with multiple transcription factor binding sites, the 3' UTR, an in-frame His6, and a floxed neo selection cassette was inserted into intron 1 of the mouse Igh-2 C alpha locus. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to transgenic Cre recombinase expressing mice to remove the neo selection cassette. The mice were then backcrossed to BALB/cAnPt for seven generations.
|Expressed Gene||Myc, myelocytomatosis oncogene, mouse, laboratory|
|Site of Expression|
|Allele Name||targeted mutation 1, Siegfried Janz|
|Allele Type||Targeted (Inserted expressed sequence)|
|Allele Synonym(s)||IgH-MycCα; IgH-MycCalpha; iMycCalpha|
|Gene Symbol and Name||Igha, immunoglobulin heavy constant alpha|
|Gene Synonym(s)||IgA; Igh-2; Igh-2; immunoglobulin heavy chain 2 (serum IgA)|
|Expressed Gene||Myc, myelocytomatosis oncogene, mouse, laboratory|
|Strain of Origin||129S1/Sv-Oca2<+> Tyr<+> Kitl<+>|
|Molecular Note||A construct consisting of a 1.5 kb of segment of genomic 5' flanking sequence from the mouse Myc gene, the first noncoding exon with the P1/P2 promoters, a mouse Myc intron-less cDNA clone with an in-frame artificial histidine tag on the 3' end, a short segment of the 3' UTR including the major polyadenylationsite, and a loxP flanked neo cassettte was inserted in opposite transcriptional orientation into intron 1 of the C alpha locus via homologous recombination. The neo cassette was subsequently removed by mating to cre transgenic mice.|
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
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The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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