These RAG-2fl mice harbor loxP sites flanking the entire coding region of the Rag2 (recombination activating gene 2) locus. When bred to mice that express Cre recombinase, the resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These RAG-2fl may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.
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Mice homozygous for the RAG-2fl allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the entire RAG-2 protein) deleted in the cre-expressing tissue(s). These RAG-2fl mice may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.
For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studies of B and T cell development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to insert a loxP-flanked neomycin resistance gene on one side, and a third loxP site on the other side of exon 3 of the targeted gene. This construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing vector to remove the selection cassette. ES cells in which the neomycin resistance cassette was deleted (leaving a single loxP site upstream, and a single loxP downstream of exon 3) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to generate RAG-2fl mice. These RAG-2fl mice were bred with transgenic mice on mixed genetic background, and then bred with mutant mice on a C57BL/6 genetic background for 8 generations. The donating investigator reports that both the transgene and other mutant gene were bred out of these RAG-2fl mice prior to arrival at The Jackson Laboratory (as Stock No. 008309). Upon arrival, some mice were backcrossed to BALB/cByJ (Stock No. 001026) using a marker-assisted approach to generate this congenic strain (Stock No. 008338).
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Rag2, recombination activating gene 2|
|Gene Synonym(s)||RAG-2; Rag-2; Rag-2|
|Strain of Origin||B6.Cg-Thy1|
|Molecular Note||LoxP sites were inserted to flank the coding region of the gene. The inserted sites had no effect on the normal function of the gene.|
|Mutations Made By|| |
Klaus Rajewsky, Max-Delbruck-Ctr. for Molecular Medicine
Mutant mice were bred to BALB/cByJ (Stock No. 001026) using a marker-assisted approach to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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