These RAG-2fl mice harbor loxP sites flanking the entire coding region of the Rag2 (recombination activating gene 2) locus. When bred to mice that express Cre recombinase, the resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These RAG-2fl may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Rag2 | recombination activating gene 2 |
Mice homozygous for the RAG-2fl allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the entire RAG-2 protein) deleted in the cre-expressing tissue(s). These RAG-2fl mice may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.
For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studies of B and T cell development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to insert a loxP-flanked neomycin resistance gene on one side, and a third loxP site on the other side of exon 3 of the targeted gene. This construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing vector to remove the selection cassette. ES cells in which the neomycin resistance cassette was deleted (leaving a single loxP site upstream, and a single loxP downstream of exon 3) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to generate RAG-2fl mice. These RAG-2fl mice were bred with transgenic mice on mixed genetic background, and then bred with mutant mice on a C57BL/6 genetic background for 8 generations. The donating investigator reports that both the transgene and other mutant gene were bred out of these RAG-2fl mice prior to arrival at The Jackson Laboratory (as Stock No. 008309). Upon arrival, some mice were backcrossed to BALB/cByJ (Stock No. 001026) using a marker-assisted approach to generate this congenic strain (Stock No. 008338).
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | RAG-2fl |
Gene Symbol and Name | Rag2, recombination activating gene 2 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 2 |
Molecular Note | LoxP sites were inserted to flank the coding region of the gene. The inserted sites had no effect on the normal function of the gene. |
Mutations Made By | Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine |
Mutant mice were bred to BALB/cByJ (Stock No. 001026) using a marker-assisted approach to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
When using the CByJ.B6(Cg)-Rag2tm1Cgn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008338 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rag2<tm1Cgn> |
Frozen Mouse Embryo | CByJ.B6(Cg)-Rag2<tm1Cgn>/J | $2595.00 |
Frozen Mouse Embryo | CByJ.B6(Cg)-Rag2<tm1Cgn>/J | $2595.00 |
Frozen Mouse Embryo | CByJ.B6(Cg)-Rag2<tm1Cgn>/J | $3373.50 |
Frozen Mouse Embryo | CByJ.B6(Cg)-Rag2<tm1Cgn>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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