B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J

Stock number: 008299
Research areas: Neurobiology Research, Research Tools

Description

The transgene in these mice is composed of a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Hemizygous mice exhibit fluorescence in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord.

Detailed description

The transgene in these mice is composed of a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Hemizygous mice exhibit fluorescence in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord.

Development

A vector carrying a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein was created. The transgenic vector was probably injected into the C57BL/6 x BALB/c(?) F1 blastocysts. Offspring showing germline transmission were crossed to C57BL/6NCrl for four generations by the donating laboratory.

Allele

Symbol: Tg(NEFL-EYFP/Nefh)40Gsn
Name: transgene insertion 40, Lawrence SB Goldstein
MGI accession ID: MGI:3778525
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(NEFL-EYFP/Nefh)40Gsn
Marker name: transgene insertion 40, Lawrence SB Goldstein
Chromosome: UN
Strain of origin: (C57BL/6 x BALB/c)F1
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: axonal fibers of sciatic nerves, optical nerves, and spinal cord
Molecular note: The transgene construct was created from a vector carrying a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Fluorescence was detected in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord.