The transgene in these mice is composed of a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Hemizygous mice exhibit fluorescence in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord.
Dr. Lawrence S.B. Goldstein, UCSD
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
The transgene in these mice is composed of a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Hemizygous mice exhibit fluorescence in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord.
A vector carrying a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein was created. The transgenic vector was probably injected into the C57BL/6 x BALB/c(?) F1 blastocysts. Offspring showing germline transmission were crossed to C57BL/6NCrl for four generations by the donating laboratory.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
---|---|
Site of Expression | axonal fibers of sciatic nerves, optical nerves, and spinal cord |
Allele Name | transgene insertion 40, Lawrence SB Goldstein |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | EYFP-NFH |
Gene Symbol and Name | Tg(NEFL-EYFP/Nefh)40Gsn, transgene insertion 40, Lawrence SB Goldstein |
Gene Synonym(s) | |
Promoter | NEFL, neurofilament light, human |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | axonal fibers of sciatic nerves, optical nerves, and spinal cord |
Strain of Origin | (C57BL/6 x BALB/c)F1 |
Chromosome | UN |
Molecular Note | The transgene construct was created from a vector carrying a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Fluorescence was detected in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord. |
When maintained as live colonies, hemizygotes may be bred to wildtype mice. It is not known if homozygotes are viable or fertile.
When using the B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008299 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(NEFL-EYFP/Nefh)40Gsn |
Frozen Mouse Embryo | B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.