This spontaneous mutation provides a model for craniofacial developmental defects including low set ears and twisted snout.
Read More +Genetic Background | Generation |
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000355 CXB5/ByJ |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Not Specified) | malf | malformed ear |
malf homozygotes have craniofacial defects that include primarily misplaced ears and, in some instances, significant asymmetry of the snout. The ear pinnae can be smaller than normal, misshapen, and positioned lower than normal and this can affect one or both ears. The phenotype ranges from subtle to pronounced. Of 37 mutants 28 had the left pinna affected, 8 had the right pinna affected and 1 had both affected. The parental phenotype does not appear to instruct which ear is affected. Of 117 offspring from homozygous x heterozygous or heterozygous x homozygous crosses only 39 (33%) were clearly mutant, indicative of either incomplete penetrance or partial embryonic lethality. In a mapping cross to C3H/HeSnJ only nine mutants were identified in one hundred seven F2 hybrids (8.4%). No mutants were identified in twenty-eight F2 hybrids with FVB/NJ, thirty-four F2 hybrids with CAST/EiJ, or nine F2 hybrids with A/J.
The malformed ear mutation arose spontaneously in the CXB5/ByJ strain at The Jackson Laboratory in 2005 when that strain was at generation F117. It was maintained by sibling intercrossing, primarily heterozygotes with homozygotes and vice versa. Sperm was cryopreserved from homozygous males at generation F6 and cryo-recovery will use BALB/cByJ oocytes since this has provided a successful recovery.
Some homozygotes with a twisted snout require tooth trimming due to malocclusion.
When using the malformed ear mouse strain in a publication, please cite the originating article(s) and include JAX stock #008260 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for malf |
Frozen Mouse Embryo | CXB5/ByJ-malf/J | $2595.00 |
Frozen Mouse Embryo | CXB5/ByJ-malf/J | $2595.00 |
Frozen Mouse Embryo | CXB5/ByJ-malf/J | $3373.50 |
Frozen Mouse Embryo | CXB5/ByJ-malf/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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