129;B6-Tff3^tm1Dkpy/J

Stock number: 008243
Research areas: Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

These intestinal trefoil factor (ITF or Tff3) mutant mice may be useful in studying gastrointestinal tract injury (including inflammatory bowel diseases), maintenance of the mucosal barrier, migration and turnover of the intestinal epithelium, and therapies for colon cancer.

Detailed description

Mice homozygous for this intestinal trefoil factor (ITF or Tff3) mutant allele are viable and fertile with no RNA or protein expression of the targeted gene in the gastrointestinal tract. Homozygous mice exhibit impaired physiological migration of intestinal epithelium to the mucosal surface, have impaired mucosal healing and increased susceptibility to dextran sulfate sodium (DSS)- induced colitis, and are more susceptible to chemotherapy and radiation-induced mucositis. These intestinal trefoil factor (ITF or Tff3) mutant mice may be useful in studying gastrointestinal tract injury (including inflammatory bowel diseases), maintenance of the mucosal barrier, migration and turnover of the intestinal epithelium, and therapies for colon cancer.

Development

A targeting vector was designed to replace exon 2 of the targeted gene with a neomycin resistance gene cassette. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were identified. These mutant mice were maintained on a "129" genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred with 129S1/SvImJ mice for at least one generation to establish the colony.

A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory on the rederived mice revealed six segregating markers (each on a different chromosome). All six loci suggest this strain is on a mixed 129;C57BL/6 genetic background (July 2009).

Allele

Symbol: Tff3^tm1Dkpy
Name: targeted mutation 1, Daniel K Podolsky
MGI accession ID: MGI:2179219
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tff3
Marker name: trefoil factor 3, intestinal
Chromosome: 17
Strain of origin: 129S4/SvJae
Molecular note: Exon 2 of this gene was replaced with a neo cassette via homologous recombination resulting in the deletion of the trefoil domain. Absence of gene expression in the gastrointestinal tract of homozygous mutant animals was confirmed by Northern blot analysis and immunohistochemistry.