B6.129-Nrgn^tm1Kph/J

Stock number: 008233
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

These neurogranin mutant mice may be useful for neurological studies involving memory and learning, neuronal signaling pathways (including calmodulin, alpha-CaMKII, protein kinase A, protein kinase C, MAPK, and CREB), attention deficit-hyperactivity disorder (ADHD), and schizophrenia.

Detailed description

Homozygous neurogranin-deficient (Ng-/-) mice are viable and fertile (although the donating investigator reports that homozygous females do not nurse their pups as well as wildtype or heterozygous mothers). Homozygotes have no mRNA or protein from the targeted gene observed in brain tissues. Expression of lacZ is observed in a manner consistent with the endogenous gene. Ng-/- mice exhibit impaired spatial learning, altered hippocampal short- and long-term plasticity (including long-term potentiation induction), and decreased activated CaMKII. Heterozygotes show similar, yet milder, effects. These neurogranin- (Ng or RC3)-mutant mice may be useful for neurological studies involving memory and learning, neuronal signaling pathways (including calmodulin, alpha-CaMKII, protein kinase A, protein kinase C, MAPK, and CREB), attention deficit-hyperactivity disorder (ADHD), and schizophrenia.

Development

A targeting vector was designed to replace the first five amino acids in exon 1 and the adjoining 98 bp of the first intron of the targeted gene with the lacZ and neomycin resistance genes. This construct was electroporated into 129/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6 females to generate mutant mice. Next, mutant mice were backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory.

Allele

Symbol: Nrgn^tm1Kph
Name: targeted mutation 1, Kuo-Ping Huang
MGI accession ID: MGI:1927195
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Nrgn
Marker name: neurogranin
Chromosome: 9
Strain of origin: 129/Sv
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is observed in a manner consistent with the endogenous gene
Molecular note: Insertion of a LacZ gene in frame into the first coding exon, followed by a neomycin cassette. No NRGN immunoreactivity was detected in brain sections derived from homozygous mutant mice. X-gal staining in homozygous mutant mice corresponded with the NRGN positive staining in wild-type mice.