These mice express tTA (tetracycline regulated transactivator) from the endogenous Pparg locus. Heterozygotes exhibit mild lipodystrophy with pale, unilocular brown adipocytes, hypertrophy of brown fat, reduced subcutaneous fat, reduced gonadal fat pads, mild hepatomegaly and develop insulin resistance and dyslipidemia. Treatment with doxycycline prevents this phenotype. This mutant mouse strain may be useful in studies of lipodystrophy.
Yaacov Barak, University of Pittsburgh
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Transactivator) | Pparg | peroxisome proliferator activated receptor gamma |
These mice express tTA (tetracycline regulated transactivator) from the endogenous Pparg locus. A fusion transcript (including the 5' portion of Pparg, IRES and tTA (AF1-IRES-tTA)) and a wild-type Pparg transcript are detected by Northem blot analysis of epididymal fat pads from heterozygotes. Mice that are heterozygous for this targeted mutation exhibit a similar but milder phenotype than that observed in Pparg-Ldi mice (129-Ppargtm2Yba/J, Stock No. 008079). Heterozygotes exhibit mild lipodystrophy with pale, unilocular brown adipocytes, hypertrophy of brown fat, reduced subcutaneous fat, gonadal fat pads smaller than wild-type and mild hepatomegaly. Mutant mice develop insulin resistance and dyslipidemia. Treatment with doxycycline prevents this phenotype. Mice that are homozygous for the targeted mutation are not viable. This mutant mouse strain may be useful in studies of lipodystrophy.
A targeting vector containing an IRES sequence, tTA (tetracycline regulated transactivator) gene, PGK-neo cassette, and synthetic polyA, was used to replace exon 2. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ mice, and then backcrossed to C57BL/6J for 7 generations. The Donating Investigator maintained the strain by crossing heterozygote males to wildtype females.
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
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Site of Expression | Expression of tTA from the endogenous Pparg locus is detected in adipose tissue. |
Allele Name | targeted mutation 3, Yaacob Barak |
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Allele Type | Targeted (Transactivator) |
Allele Synonym(s) | Ppargtm3Yba; PpargtTA |
Gene Symbol and Name | Pparg, peroxisome proliferator activated receptor gamma |
Gene Synonym(s) | |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | Expression of tTA from the endogenous Pparg locus is detected in adipose tissue. |
Strain of Origin | Not Specified |
Chromosome | 6 |
Molecular Note | Exon 2 was replaced with an IRES, tTA and neo cassette. While expression is modest in adipose tissue, it is poor in the liver and placenta. |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are not viable. The Donating Investigator maintained the strain by crossing heterozygous males to wildtype C57BL/6J females. Heterozygous females produce sickly pups that often do not reproduce.
When using the B6.129S4-Ppargtm3(tTA)Yba/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008227 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Pparg<tm3Yba> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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