These IGFBP-2 overexpressing transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).
Ajay Shah, King's College London
Mice hemizygous for the IGFBP-2 transgene are viable and fertile with no reported gross morphological or developmental changes. Transgenic mice overexpress human IGFBP-2 (hIGFBP-2), with hIGFBP-2 mRNA detected in a variety of organs and tissues, including adipose tissue. Overexpression of hIGFBP-2 is associated with reduced susceptibility to obesity and improved insulin sensitivity; transgenic mice are protected from glucose intolerance and increased blood pressure with age, and are also resistant to obesity and insulin resistance on a high fat diet. The phenotype of hIGFBP-2 overexpressing mice may vary between male and female mice. These IGFBP-2 transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As these mice were originally characterized on the FVB/N genetic background, it should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Founder mice (line tg1) were bred to FVB/N mice. After this, the Donating Investigator reports that the IGFBP-2 transgenic mice were backcrossed to C57BL/6J mice for at least 9 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation. SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed these mice are approximately 94% congenic on a C57BL/6 genetic background, which is less than the expected incipient congenic background.
|Expressed Gene||IGFBP2, insulin like growth factor binding protein 2, human|
|Site of Expression|
|Allele Name||transgene insertion 1, John Miell|
|Allele Type||Transgenic (Humanized sequence, Inserted expressed sequence)|
|Allele Synonym(s)||line tg1|
|Gene Symbol and Name||Tg(IGFBP2)1Miel, transgene insertion 1, John Miell|
|Gene Synonym(s)||line tg1|
|Promoter||IGFBP2, insulin like growth factor binding protein 2, human|
|Expressed Gene||IGFBP2, insulin like growth factor binding protein 2, human|
|Strain of Origin||FVB/N|
|Molecular Note||The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Two lines tg1 and tg2 were established and line tg1 was analyzed in detail.|
|Mutations Made By|| |
John Miell, University Hospital Lewisham
When maintaining a live colony, carrier mice may be bred to wildtype or C57BL/6J inbred mice.
When using the B6;FVB-Tg(IGFBP2)1Miel/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008222 in your Materials and Methods section.
|Hemizygous or Non carrier for Tg(IGFBP2)1Miel|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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