JAX Mouse Strain Datasheet - 008222

B6;FVB-Tg(IGFBP2)1Miel/J

Stock No:: 008222

Cryo Recovery

Overview

These IGFBP-2 overexpressing transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).

Donating Investigator

Ajay Shah, King's College London

Genetic overview

Genetic Background	Generation

Tg(IGFBP2)1Miel

Allele Type
Transgenic (Humanized sequence, Inserted expressed sequence)

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Research Applications

Cardiovascular Research
Diabetes and Obesity Research
Metabolism Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice hemizygous for the IGFBP-2 transgene are viable and fertile with no reported gross morphological or developmental changes. Transgenic mice overexpress human IGFBP-2 (hIGFBP-2), with hIGFBP-2 mRNA detected in a variety of organs and tissues, including adipose tissue. Overexpression of hIGFBP-2 is associated with reduced susceptibility to obesity and improved insulin sensitivity; transgenic mice are protected from glucose intolerance and increased blood pressure with age, and are also resistant to obesity and insulin resistance on a high fat diet. The phenotype of hIGFBP-2 overexpressing mice may vary between male and female mice. These IGFBP-2 transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As these mice were originally characterized on the FVB/N genetic background, it should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Founder mice (line tg1) were bred to FVB/N mice. After this, the Donating Investigator reports that the IGFBP-2 transgenic mice were backcrossed to C57BL/6J mice for at least 9 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation. SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed these mice are approximately 94% congenic on a C57BL/6 genetic background, which is less than the expected incipient congenic background.

Expression Data

Expressed Gene	IGFBP2, insulin like growth factor binding protein 2, human
Site of Expression

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wheatcroft SB; Kearney MT; Shah AM; Ezzat VA; Miell JR; Modo M; Williams SC; Cawthorn WP; Medina-Gomez G; Vidal-Puig A; Sethi JK; Crossey PA. 2007. IGF-binding protein-2 protects against the development of obesity and insulin resistance. Diabetes 56(2):285-94. PubMed: 17259371 MGI: J:121961

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Genetics

Tg(IGFBP2)1Miel

Allele Symbol: Tg(IGFBP2)1Miel

Allele Name	transgene insertion 1, John Miell
Allele Type	Transgenic (Humanized sequence, Inserted expressed sequence)
Allele Synonym(s)	line tg1
Gene Symbol and Name	Tg(IGFBP2)1Miel, transgene insertion 1, John Miell
Gene Synonym(s)	line tg1
Promoter	IGFBP2, insulin like growth factor binding protein 2, human
Expressed Gene	IGFBP2, insulin like growth factor binding protein 2, human
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Two lines tg1 and tg2 were established and line tg1 was analyzed in detail.
Mutations Made By	John Miell, University Hospital Lewisham

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cardiovascular Research
- Other
Diabetes and Obesity Research
- Insulin Receptors and Growth Factors
- Obesity With Diabetes
- Obesity Without Diabetes
- Type 2 Diabetes (NIDDM)
Metabolism Research
Research Tools
- Cardiovascular Research
- Diabetes and Obesity Research
- Metabolism Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(IGFBP2)1Miel/0
FVB/N-Tg(IGFBP2)1Miel

growth/size/body region phenotype

decreased susceptibility to diet-induced obesity
- on high-fat diet for 32 weeks, mutants remain lean with no significant increase in size of fat depots, whereas wild-type mice have markedly increased size of fat depots when examined at 40 weeks

slow postnatal weight gain
- on a high-fat/high-calorie diet for 32 weeks from 8 weeks of age, mutants show attenuated weight gain relative to wild-type mice

adipose tissue phenotype

abnormal abdominal fat pad morphology
- transgenic mice on high-fat diet for 32 weeks have significant reduction intraabdominal fat than wild-type mice; similarly, regular chow-fed mutants have less abdominal fat than diet-matched controls

abnormal fat cell morphology
- adipocytes are smaller than in wild-type mice, on both regular (1390 vs. 2100 um² mean cell area) and high-fat chow diets (2800 vs. 5900 um² mean cell area)

decreased total body fat amount
- on high-fat diet for 32 weeks, mutants have smaller fat depots than wild-type mice on the same diet when examined at 40 weeks

cardiovascular system phenotype

cardiovascular system phenotype
- mutants are protected from age-related hypertension observed in aged wild-type mice

decreased systemic arterial systolic blood pressure
- wild-type mice at 40 weeks show increased systolic blood pressure compared to transgenic mice on both regular chow and high-fat diets

liver/biliary system phenotype

liver/biliary system phenotype
- on high-fat diet for 32 weeks, mutants do not exhibit steatosis, whereas wild-type livers have macroscopic appearance compatible with increased fat content
- on high-fat diet for 32 weeks, mutants exhibit lower liver weight relative to controls when examined at 40 weeks

homeostasis/metabolism phenotype

abnormal circulating insulin level
- in response to carbohydrate challenge, no siginificant increase in plasma insulin level is observed compared to elevated levels displayed by wild-type mice

abnormal glucose homeostasis
- in response to IGF-I, transgenic mice show an attenuated hypoglycemic response; plasma glucose levels decrease by ~half the value exhibited by wild-type mice

decreased circulating glucose level
- by 4-6 months of age, transgenic mice display lower nonfasted glucose levels than wild-type littermates
- increase in blood glucose of transgenic mice on high-fat diet is significantly blunted relative to wild-type

decreased circulating leptin level
- leptin level increase is blunted in transgenic mice on high-fat diet, compared to wild-type

decreased circulating triglyceride level
- on high-fat diet, plasma triglyceride levels are significantly lower than in wild-type

decreased susceptibility to diet-induced obesity
- on high-fat diet for 32 weeks, mutants remain lean with no significant increase in size of fat depots, whereas wild-type mice have markedly increased size of fat depots when examined at 40 weeks

homeostasis/metabolism phenotype
- at 8 weeks of age, blood glucose and insulin levels are similar to wild-type mice
- insulin levels are similar to wild-type at 4-6 months of age
- on high-fat diet, total energy intake is not significantly different from wild-type

improved glucose tolerance
- mice are protected against the age-related decline in glucose tolerance displayed by wild-type mice between 8 and 40 weeks
- on both regular and high-fat diets, blood glucose of transgenic mice is significantly lower after glucose tolerance test than in wild-type
- on high-fat diet, transgenic mice are protected from development of glucose intolerance compared to wild-type

increased insulin sensitivity
- hypoglycemic response to exogenous insulin is greater in 40-week old mice compared to controls

References

Wheatcroft SB; Kearney MT; Shah AM; Ezzat VA; Miell JR; Modo M; Williams SC; Cawthorn WP; Medina-Gomez G; Vidal-Puig A; Sethi JK; Crossey PA. 2007. IGF-binding protein-2 protects against the development of obesity and insulin resistance. Diabetes 56(2):285-94. PubMed: 17259371 MGI: J:121961

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(IGFBP2)1Miel

Allele Symbol: Tg(IGFBP2)1Miel

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(IGFBP2)1Miel/0FVB/N-Tg(IGFBP2)1Miel

growth/size/body region phenotype

adipose tissue phenotype

cardiovascular system phenotype

liver/biliary system phenotype

homeostasis/metabolism phenotype

References

Genotyping Protocols

Breeding Considerations

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Genotype: Tg(IGFBP2)1Miel/0
FVB/N-Tg(IGFBP2)1Miel