These IGFBP-2 overexpressing transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).
Ajay Shah, King's College London
Mice hemizygous for the IGFBP-2 transgene are viable and fertile with no reported gross morphological or developmental changes. Transgenic mice overexpress human IGFBP-2 (hIGFBP-2), with hIGFBP-2 mRNA detected in a variety of organs and tissues, including adipose tissue. Overexpression of hIGFBP-2 is associated with reduced susceptibility to obesity and improved insulin sensitivity; transgenic mice are protected from glucose intolerance and increased blood pressure with age, and are also resistant to obesity and insulin resistance on a high fat diet. The phenotype of hIGFBP-2 overexpressing mice may vary between male and female mice. These IGFBP-2 transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As these mice were originally characterized on the FVB/N genetic background, it should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Founder mice (line tg1) were bred to FVB/N mice. After this, the Donating Investigator reports that the IGFBP-2 transgenic mice were backcrossed to C57BL/6J mice for at least 9 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation. SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed these mice are approximately 94% congenic on a C57BL/6 genetic background, which is less than the expected incipient congenic background.
|Expressed Gene||IGFBP2, insulin like growth factor binding protein 2, human|
|Site of Expression|
|Allele Name||transgene insertion 1, John Miell|
|Allele Type||Transgenic (Humanized sequence, Inserted expressed sequence)|
|Allele Synonym(s)||line tg1|
|Gene Symbol and Name||Tg(IGFBP2)1Miel, transgene insertion 1, John Miell|
|Gene Synonym(s)||line tg1|
|Promoter||IGFBP2, insulin like growth factor binding protein 2, human|
|Expressed Gene||IGFBP2, insulin like growth factor binding protein 2, human|
|Strain of Origin||FVB/N|
|Molecular Note||The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Two lines tg1 and tg2 were established and line tg1 was analyzed in detail.|
|Mutations Made By|| |
John Miell, University Hospital Lewisham
When maintaining a live colony, carrier mice may be bred to wildtype or C57BL/6J inbred mice.
When using the B6;FVB-Tg(IGFBP2)1Miel/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008222 in your Materials and Methods section.
|Hemizygous or Non carrier for Tg(IGFBP2)1Miel|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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