These hIGFBP-1 (insulin-like growth factor binding protein 1, human) transgenic mice may be useful in studying metabolic and vascular homeostasis, diabetes, and the role of insulin-like growth factor binding protein in placentation and preeclampsia.
Ajay Shah, King's College London
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for the hIGFBP-1 transgene are viable and fertile with no reported gross morphological or developmental changes. The hIGFBP-1 transgene encompasses the entire human IGFBP-1 structural gene and its regulatory sequences, allowing transgene expression of IGFBP-1 to remain responsive to normal hormonal regulation. Transgenic mice overexpress hIGFBP-1, with hIGFBP-1 mRNA expression in a tissue-specific fashion more similar to the human pattern than the murine pattern. Fasting transgenic mice have elevated total serum IGFBP-1 levels that fluctuate according to nutritional status (as they do in humans), and exhibit postprandial hyperinsulinemia with preservation of normal glucocompetence and insulin sensitivity. Transgenic mice also have significantly greater hyperinsulinemic response to glucose challenge and cardiovascular abnormalities in response to carbohydrate load and vasoconstrictors. Transgenic mice exhibit fasting hyperglycemia and hyperinsulinemia and glucose intolerance in later life. The phenotype of hIGFBP-1 overexpressing mice may vary between male and female mice. These hIGFBP-1 transgenic mice may be useful in studying metabolic and vascular homeostasis, diabetes, and the role of insulin-like growth factor binding protein in placentation and preeclampsia.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As these mice were originally characterized on both C57BL/CBA mixed and CBA congenic backgrounds, it should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The hIGFBP-1 transgene (isolated from a human cosmid clone) contains the entire structural IGFBP1 gene and all the cis acting sequences necessary to direct appropriate tissue-specific and regulated gene expression. This 35 kb transgene was microinjected into the male pronucleus of C57BL/CBA fertilized oocytes. Founder mice (line c1:2-2) were bred to CBA/Ca and the transgene copy number was found to be between 8 and 20. After this, hIGFBP-1 transgenic mice were backcrossed to C57BL/6J mice for at least 9 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation.
Expressed Gene | IGFBP1, insulin like growth factor binding protein 1, human |
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Site of Expression |
Allele Name | transgene insertion 2, John Miell |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | c1:2-2; hIGFBP-1 (line c1:2-2) |
Gene Symbol and Name | Tg(IGFBP1)2Miel, transgene insertion 2, John Miell |
Gene Synonym(s) | |
Promoter | IGFBP1, insulin like growth factor binding protein 1, human |
Expressed Gene | IGFBP1, insulin like growth factor binding protein 1, human |
Strain of Origin | C57BL and CBA |
Chromosome | UN |
Molecular Note | The hIGFBP-1 transgene (isolated from a human cosmid clone) contains the entire structural IGFBP1 gene and all the cis acting sequences necessary to direct appropriate tissue-specific and regulated gene expression. Founder mice (line c1:2-2) were bred to CBA/Ca and the transgene copy number was found to be between 8 and 20. |
Mutations Made By | John Miell, University Hospital Lewisham |
When maintaining a live colony, carrier mice may be bred to wildtype or C57BL/6J inbred mice.
When using the B6.Cg-Tg(IGFBP1)2Miel/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008221 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(IGFBP1)2Miel |
Frozen Mouse Embryo | B6.Cg-Tg(IGFBP1)2Miel/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(IGFBP1)2Miel/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(IGFBP1)2Miel/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(IGFBP1)2Miel/J Frozen Embryo | $3373.50 |
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