Male mice of this knock-out mutation exhibit abnormal sperm morphology and motility, and are infertile. These mice may be useful in studies of acrosomal biogenesis and spermiogenesis.
Dr. Martin Matzuk, Baylor College of Medicine
Female mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous males are infertile due to severe abnormal spermatozoa morphology and diminished sperm motility. Heterozygous males exhibit terato-asthenozoospermia (increase in the percentage of abnormal sperm) and globozoospermia, but are fertile with normal fecundity.
Electron microscopic examination of sperm reveals ultrastructural abnormalties. No gene product (mRNA or protein) is detected by Northern or Western blot analysis or testicular tissue and sperm. This mutant mouse strain may be useful in studies of acrosomal biogenesis and spermiogenesis.
A targeting vector containing a PgkHPRT cassette was used to disrupt exons 1 and 2, which encode the signal peptide region. The construct was electroporated into 129S7/SvEvBrd-Hprt1b-m2 derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. The mice were maintained on a B6;129S7 background before arriving at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Martin M Matzuk|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Zpbp1-; Zpbp1tm1Zuk|
|Gene Symbol and Name||Zpbp, zona pellucida binding protein|
|Strain of Origin||129S7/SvEvBrd-Hprtb-m2|
|Molecular Note||Exons 1 and 2 were replaced with a PgkHPRT cassette via homologous recombination. This resulted in deletion of the signal peptide encoding region. Northern and Western blot analysis confirmed the absence of RNA transcript and protein in the testes.|
|Mutations Made By|| |
Yi-Nan Lin, IBMS, Academia Sinica
When maintaining a live colony, these mice can be bred as homozygous females and heterozygous males. Homozygous females are fertile, homozygous males are infertile due to severe abnormal spermatozoa morphology and diminished sperm motility.
When using the Zpbp1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008219 in your Materials and Methods section.