These lacZ reporter mutant mice harbor a β-galactosidase "knock-in" mutation that also abolishes endogenous GLI-Kruppel family member GLI1 (Gli1) gene function, and may be useful for studying Hedgehog/Sonic Hedgehog signaling in axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.
Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Gli1 | GLI-Kruppel family member GLI1 |
Mice homozygous for the Gli1lz (or Gli1lacZ) allele are viable and semi-fertile, with a "knock-in" of β-galactosidase (lacZ) inserted into the first coding exon (exon 2) and replacing the genomic fragment encoding the entire N-terminal and zinc-finger domains of the targeted locus (exons 2-7); abolishing endogenous gene function even if alternative splicing occurs. Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern indistinguishable from wildtype gene mRNA expression. As Gli1 transcription is a readout of high level Hedgehog signaling, these Gli1lz (or Gli1lacZ) mice may be useful for studying Hedgehog/Sonic Hedgehog signaling in axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.
A targeting vector was designed to insert a nuclear localized β-galactosidase (lacZ) cDNA and loxP-flanked, reverse-oriented neo cassette into the first coding exon (exon 2) and delete the coding sequences in exons two to seven of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129SvEv (Taconic) mice. Next, mutant mice were bred to TK-Cre transgenic mice (129SvEv genetic background) to remove the floxed neo cassette. The resulting Gli1lz (or Gli1lacZ) offspring were selectively bred to remove the TK-Cre transgene and then subsequently maintained by breeding to Swiss Webster mice and also interbreeding for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | LacZ expression replaces that of the targeted gene in a pattern indistinguishable from that of the wildtype. |
Allele Name | targeted mutation 2, Alexandra L Joyner |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Gli1LacZ; Gli1lz; Gli1lzki; Gli1nlacZ; Gli1null; Gli1-LacZ |
Gene Symbol and Name | Gli1, GLI-Kruppel family member GLI1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | LacZ expression replaces that of the targeted gene in a pattern indistinguishable from that of the wildtype. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 10 |
Molecular Note | The gene was disrupted by replacement of a genomic region encoding the Gli N-terminal and zinc finger domains with a floxed-neo cassette and a lacZ having a nuclear localizing signal. The floxed-neo cassette was subsequently removed by crossing mutant animals to a cre deleter strain. The lacZ expression pattern in homozygous mutant embryos is identical to that of wild-type Gli as determined by immunohistochemical analysis. |
Mutations Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center |
When maintaining a live colony, heterozygous mice may be bred with wildtype siblings. The donating investigator reports that homozygous mice are viable but semi-fertile.
When using the Gli1lz mouse strain in a publication, please cite the originating article(s) and include JAX stock #008211 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Gli1<tm2Alj> |
Frozen Mouse Embryo | STOCK Gli1<tm2Alj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gli1<tm2Alj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gli1<tm2Alj>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gli1<tm2Alj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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