Overview

Also Known As: Gli1^lz

These lacZ reporter mutant mice harbor a β-galactosidase "knock-in" mutation that also abolishes endogenous GLI-Kruppel family member GLI1 (Gli1) gene function, and may be useful for studying Hedgehog/Sonic Hedgehog signaling in axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.

Donating Investigator

Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center

Genetic overview

Genetic Background	Generation

Gli1^tm2Alj

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Gli1	GLI-Kruppel family member GLI1

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Research Applications

Research Tools
Developmental Biology Research
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the Gli1^lz (or Gli1^lacZ) allele are viable and semi-fertile, with a "knock-in" of β-galactosidase (lacZ) inserted into the first coding exon (exon 2) and replacing the genomic fragment encoding the entire N-terminal and zinc-finger domains of the targeted locus (exons 2-7); abolishing endogenous gene function even if alternative splicing occurs. Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern indistinguishable from wildtype gene mRNA expression. As Gli1 transcription is a readout of high level Hedgehog signaling, these Gli1^lz (or Gli1^lacZ) mice may be useful for studying Hedgehog/Sonic Hedgehog signaling in axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.

Development

A targeting vector was designed to insert a nuclear localized β-galactosidase (lacZ) cDNA and loxP-flanked, reverse-oriented neo cassette into the first coding exon (exon 2) and delete the coding sequences in exons two to seven of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129SvEv (Taconic) mice. Next, mutant mice were bred to TK-Cre transgenic mice (129SvEv genetic background) to remove the floxed neo cassette. The resulting Gli1^lz (or Gli1^lacZ) offspring were selectively bred to remove the TK-Cre transgene and then subsequently maintained by breeding to Swiss Webster mice and also interbreeding for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	LacZ expression replaces that of the targeted gene in a pattern indistinguishable from that of the wildtype.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Bai CB; Auerbach W; Lee JS; Stephen D; Joyner AL. 2002. Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathway. Development 129(20):4753-61PubMed: 12361967 MGI: J:79392

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Genetics

Gli1^tm2Alj

Allele Symbol: Gli1^tm2Alj

Allele Name	targeted mutation 2, Alexandra L Joyner
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 2, Alexandra L Joyner; Gli1^tm2Alj
Gene Symbol and Name	Gli1, GLI-Kruppel family member GLI1
Gene Synonym(s)	zinc finger protein 5; Zfp-5; Zfp5; Zfp5; GLI; expressed sequence AV235269; Zfp-5; AV235269; Gli; PAPA8; PPD1
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	LacZ expression replaces that of the targeted gene in a pattern indistinguishable from that of the wildtype.
Strain of Origin	129S6/SvEvTac
Chromosome	10
Molecular Note	The gene was disrupted by replacement of a genomic region encoding the Gli N-terminal and zinc finger domains with a floxed-neo cassette and a lacZ having a nuclear localizing signal. The floxed-neo cassette was subsequently removed by crossing mutant animals to a cre deleter strain. The lacZ expression pattern in homozygous mutant embryos is identical to that of wild-type Gli as determined by immunohistochemical analysis.
Mutations Made By	Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- lacZ Expression
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers
- Neurobiology Research
  - cell marker
Developmental Biology Research
- Limb Patterning Defects
Neurobiology Research
- lacZ expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Gli1^tm2Alj/Gli1⁺
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster

mammalian phenotype

reproductive system phenotype
- Normal - embryos show normal mammary gland development

Genotype: Gli1^tm2Alj/Gli1^tm2Alj
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster

mammalian phenotype

reproductive system phenotype
- Normal - embryos show normal mammary gland development

References

Bai CB; Auerbach W; Lee JS; Stephen D; Joyner AL. 2002. Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathway. Development 129(20):4753-61PubMed: 12361967 MGI: J:79392

Additional - Gli1^tm2Alj related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Gli1^tm2AlJ
- MELT: Gli1 ^{tm2 Alj} MCA
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred with wildtype siblings. The donating investigator reports that homozygous mice are viable but semi-fertile.
- Additional Breeding and Husbandry Support
Mating System
- +/+ sibling x Heterozygote
Citation
When using the Gli1^lz mouse strain in a publication, please cite the originating article(s) and include JAX stock #008211 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Gli1<tm2Alj>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Gli1lz

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Gli1tm2Alj

Allele Symbol: Gli1tm2Alj

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gli1tm2Alj/Gli1+involves: 129S6/SvEvTac * Black Swiss * Swiss Webster

mammalian phenotype

Genotype: Gli1tm2Alj/Gli1tm2Aljinvolves: 129S6/SvEvTac * Black Swiss * Swiss Webster

mammalian phenotype

References

Additional - Gli1tm2Alj related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Gli1^lz

Gli1^tm2Alj

Allele Symbol: Gli1^tm2Alj

Genotype: Gli1^tm2Alj/Gli1⁺
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster

Genotype: Gli1^tm2Alj/Gli1^tm2Alj
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster

Additional - Gli1^tm2Alj related