These Oct4-neo mutant mice may be useful for the selection of induced pluripotent stem (iPS) cells.
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Pou5f1 | POU domain, class 5, transcription factor 1 |
Mice heterozygous for this Oct4-neo mutation are viable and fertile, harboring an IRES-GFPneo fusion cassette downstream of exon 5 of the Oct4 (Pou5f1) gene. The donating investigator reports (and attempts at The Jackson Laboratory confirm) no living homozygous animals are born. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. These Oct4-neo mutant mice may be useful for the selection of iPS cells (i.e. epigenetic reprogramming of somatic cells into pluripotent embryonic stem cells).
Of note, these Oct4-neo mutant mice may also be used in conjunction with Oct4-EGFP mice (Stock No. 008214); a similar mutant strain bearing an EGFP-expressing mutation from the Oct4 locus.
The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5 of the Oct4 (Pou5f1) gene. This construct was microinjected into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells (with sufficient neomycin-resistance activity but no visible GFP fluorescence) were injected into blastocysts. Chimeric mice were bred to C57BL/6NTac mice to establish the mutant colony. The resulting Oct4-neo mutant mice were maintained on a mixed B6;129S4 genetic background prior to arrival at The Jackson Laboratory.
Expressed Gene | neo, neomycin resistance, bacterial |
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Site of Expression | Embryonic stem cells. |
Allele Name | targeted mutation 1, Rudolf Jaenisch |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Oct4-IRES-GFPneo; Oct4-neo |
Gene Symbol and Name | Pou5f1, POU domain, class 5, transcription factor 1 |
Gene Synonym(s) | |
Expressed Gene | neo, neomycin resistance, bacterial |
Site of Expression | Embryonic stem cells. |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 17 |
General Note | When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. |
Molecular Note | The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. |
Mutations Made By | Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology |
When maintaining a live colony, heterozygous mice may be bred together or to wildtype siblings.
When using the B6;129S4-Pou5f1tm1Jae/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008204 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Pou5f1<tm1Jae> |
Frozen Mouse Embryo | B6;129S4-Pou5f1<tm1Jae>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Pou5f1<tm1Jae>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Pou5f1<tm1Jae>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Pou5f1<tm1Jae>/J Frozen Embryo | $3373.50 |
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