FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J

Stock number: 008202
Research areas: Research Tools

Description

These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.

Detailed description

These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2-M2) protein under the control of the human NPHS2 promoter, which directs expression to podocytes within kidney glomeruli. When mated to a second strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in podocytes is induced with administration of the tetracycline analog, doxycycline (dox). These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.

Development

The podocin-rtTA (or NPHS2-rtTA) construct was designed with a 2.5 kb fragment of the podocin (or NPHS2) promoter/enhancer region upstream of an optimized reverse tetracycline-controlled transactivator variant (rtTA2-M2; modified with humanized codon usage and the FFF minimal activator domains), followed by an SV40 3' UTR and polyA region. This transgene was microinjected into the pronuclei of fertilized FVB/N oocytes. Founder mice were bred to FVB/N inbred mice. This strain was maintained on the FVB/N genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.

Allele

Symbol: Tg(NPHS2-rtTA2*M2)1Jbk
Name: transgene insertion 1, Jeffrey B Kopp
MGI accession ID: MGI:3629962
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(NPHS2-rtTA2*M2)1Jbk
Marker name: transgene insertion 1, Jeffrey B Kopp
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: podocytes within kidney glomeruli
Molecular note: The rtTA gene was placed under the control of the human NPHS2 gene. The resulting transgene is expressed in the kidney when induced by doxycycline.