These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.
Jeffrey B Kopp, NIDDK, NIH (AMDCC)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2-M2) protein under the control of the human NPHS2 promoter, which directs expression to podocytes within kidney glomeruli. When mated to a second strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in podocytes is induced with administration of the tetracycline analog, doxycycline (dox). These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.
The podocin-rtTA (or NPHS2-rtTA) construct was designed with a 2.5 kb fragment of the podocin (or NPHS2) promoter/enhancer region upstream of an optimized reverse tetracycline-controlled transactivator variant (rtTA2-M2; modified with humanized codon usage and the FFF minimal activator domains), followed by an SV40 3' UTR and polyA region. This transgene was microinjected into the pronuclei of fertilized FVB/N oocytes. Founder mice were bred to FVB/N inbred mice. This strain was maintained on the FVB/N genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | podocytes within kidney glomeruli |
Allele Name | transgene insertion 1, Jeffrey B Kopp |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | 2.5P-rtTA; podocin-rtTA; Tg(NPHS2-rtTA)1Jbk |
Gene Symbol and Name | Tg(NPHS2-rtTA2*M2)1Jbk, transgene insertion 1, Jeffrey B Kopp |
Gene Synonym(s) | |
Promoter | NPHS2, NPHS2, podocin, human |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | podocytes within kidney glomeruli |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The rtTA gene was placed under the control of the human NPHS2 gene. The resulting transgene is expressed in the kidney when induced by doxycycline. |
Mutations Made By | Jeffrey Kopp, NIDDK, NIH (AMDCC) |
When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to FVB/NJ inbred mice.
When using the FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008202 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(NPHS2-rtTA2*M2)1Jbk |
Frozen Mouse Embryo | FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J | $3373.50 |
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