These Dlx5/6-Cre transgenic mice have Cre recombinase expression directed by the regulatory sequences of the zebrafish dlx5a/dlx6a genes and may be useful in generating specific deletions of floxed alleles in GABAergic in forebrain and other brain regions.
Marc Ekker, University of Ottawa
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Homozygous Dlx5/6-Cre transgenic mice are viable and fertile. Expression of Cre recombinase (Cre) is directed to differentiating and migrating forebrain GABAergic neurons during embryonic development by the I56i and I56ii enhancers from the zebrafish dlx5a/dlx6a intergenic region (with the 5' promoter region of zebrafish dlx6a in place to increase the activity of the intergenic enhancers rather than direct tissue-specific expression). When Dlx5/6-Cre transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring. These Dlx5/6-Cre transgenic mice may be useful in generating specific deletions of floxed alleles in GABAergic neurons in forebrain and other brain regions.
Luo et al. 2020 Neuron 106:37 Table 1 shows germline recombination in offspring (F2) of Cre;floxed double mutant (F1) mice bred to floxed and/or wildtype mice. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice.
This reports that Dlx5/6-Cre;floxed double mutant females bred to floxed and/or wildtype males produced some offspring with germline deletion of the floxed allele [observed from several cohorts (including up to 85.3% (29/34) of Cre negative offspring)]. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Dlx5/6-Cre males to floxed females.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. For endogenous mouse gene expression, information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.
The Dlx5/6-Cre transgene was designed with a 3.5 kb fragment from the immediate 5'-flanking region of zebrafish dlx6a gene (including part of the 5' UTR) placed immediately upstream of a Cre recombinase coding sequence, all followed by a 1.4 kb enhancer fragment from the zebrafish dlx5a/dlx6a intergenic region (containing both zebrafish I56i and I56ii enhancers). The donating investigator reports that a female founder mouse (founder #8561 on a CD1 genetic background) was bred to CD1 males and the resulting Dlx5/6-Cre mice were maintained by breeding hemizygous males with CD1 females for at least 10 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, transgenic animals were bred with C57BL/6J mice for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | GABAergic forebrain neurons |
Allele Name | transgene insertion 1, Marc Ekker |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Dlx5/6-Cre; Dlx5/6i-Cre; line 8561; Tg(dlx6a-cre)1Mars; Tg(dlx6a-cre)1Mekk |
Gene Symbol and Name | Tg(dlx5a-cre)1Mekk, transgene insertion 1, Marc Ekker |
Gene Synonym(s) | |
Promoter | dlx6a, distal-less homeobox gene 6a, Danio rerio, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | GABAergic forebrain neurons |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | A cre gene was placed under the control of the I56i and I56ii enhancer elements of zebrafish dlx5a and a 3.5kb genomic DNA fragment flanking the 5' region of the zebrafish dlx6a open reading frame and containing the 5'UTR. The dlx6a flanking region on its own cannot produce tissue specific expression but increases the activity of the enhancers to produce expression that recapitulate endogenous Dlx5 expression. Tissue specific expression was confirmed by test crosses to reporter mice strains, including Tg(ACTB-Bgeo/ALPP)1Lbe, Tg(ACTB-Bgeo/GFP)21Lbe and Gt(ROSA)26Sortm1Sor. |
Mutations Made By | Marc Ekker, University of Ottawa |
When maintaining a live colony, these mice can be bred as homozygotes.
For Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Dlx5/6-Cre males to floxed females. See Detailed Description for more details.
When using the Dlx5/6-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008199 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(dlx6a-cre)1Mekk |
Frozen Mouse Embryo | STOCK Tg(dlx5a-cre)1Mekk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(dlx5a-cre)1Mekk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(dlx5a-cre)1Mekk/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(dlx5a-cre)1Mekk/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.