Adipoqtm1Chan homozygotes exhibit endothelial dysfunction and are more susceptible to myocardial ischemia/reperfusion. These mice may be useful in studying obesity, diabetes, insulin resistance, metabolism, inflammation, leukocyte-endothelium interactions, and colitis.
Lawrence Chan, Baylor College of Medicine
Genetic Background | Generation |
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N8+pN3F25
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Adipoq | adiponectin, C1Q and collagen domain containing |
Homozygous mice are viable and fertile, with absence of targeted allele expression confirmed in adipose tissue (mRNA) and plasma (adiponectin protein). While homozygous mice have normal glucose tolerance and insulin resistance, beta-oxidation activity is significantly increased in muscle and liver. Homozygotes also have endothelial dysfunction (increased leukocyte rolling and leukocyte adhesion), are protected from DSS-induced colitis, and are more susceptible to myocardial ischemia/reperfusion. When fed a high fat diet, obese homozygotes are significantly heavier with increased insulin levels and altered insulin resistance. These adiponectin-deficient (Adipoq-/- or Adipo-/-) mice may be useful in studying obesity, diabetes, insulin resistance, metabolism, inflammation, leukocyte-endothelium interactions, and colitis.
At The Jackson Laboratory, it is our experience that breeding Adipoqtm1Chan homozygous mice together frequently results in pups with significant hair loss by wean age (3 weeks). It is not determined if this phenotype is caused by the genotype of the pups, or if the phenotype is imparted upon the pups due to the genotype of the lactating/nursing female. In most cases, the hair loss phenotype is reversed after weaning, with some pups growing a full coat by 6 weeks of age.
A targeting vector was designed to replace exon 2 of the targeted gene with a PGKneo cassette. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred with C57BL/6J to generate mutant mice. The donating investigator reported that mutant mice were backcrossed to C57BL/6J (see SNP note below) for at least eight generations prior to sending to The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. This revealed 2 markers on chromosome 15 and one marker on Chromosome 19 that are segregating for 129 suggesting an incomplete backcross.
Allele Name | targeted mutation 1, Lawrence Chan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ad-; Adipo-; ADN- |
Gene Symbol and Name | Adipoq, adiponectin, C1Q and collagen domain containing |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 16 |
Molecular Note | Exon 2 was replaced by a floxed neo cassette inserted by homologous recombination. Transcript was undetected in adipose tissue by Northern blot analysis of homozygous mutant mice. The lack of immunoreactivity by Western blot analysis using a carboxy terminal antibody indicated an absence of normal and truncated protein. |
Mutations Made By | Lawrence Chan, Baylor College of Medicine |
When maintaining a live colony, homozygous mice may be bred.
At The Jackson Laboratory, it is our experience that breeding Adipoqtm1Chan homozygous mice together frequently results in pups with significant hair loss by wean age. It is not determined if this phenotype is caused by the genotype of the pups, or if the phenotype is imparted upon the pups due to the genotype of the lactating/nursing female.
When using the Adipo- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008195 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Adipoq<tm1Chan> |
Frozen Mouse Embryo | B6;129-Adipoq<tm1Chan>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Adipoq<tm1Chan>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Adipoq<tm1Chan>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Adipoq<tm1Chan>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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