Congenic NOD mice hemizygous for the MIP-GFP transgene have EGFP fluorescence in tissues where insulin I is normally detected, specifically in pancreatic beta-cells. Transgenic mice are reported to develop diabetes at a similar or higher rate and kinetics as wildtype controls. Transgenic mice may be useful in studies of diabetes and pancreatic beta islet cell biology.
Qizhi Tang, U of California
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for this "MIP-GFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Green Fluorescent Protein under the control of mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. The human growth hormone sequence in the transgenic insert enhances expression of the EGFP but is not expressed.
The donating investigator reports NOD transgenic female mice develop insulitis and autoimmune diabetes at a similar rate and kinetics as female wildtype controls, 62% and 58% respectively, while surprisingly, transgenic males develop insulitis and diabetes at a higher rate than male wildtype controls, 65% and 10% respectively. This transgenic MIP-GFP mouse strain is useful in studies of diabetes and pancreatic beta islet cell biology.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the coding region of the Enhanced Green Fluorescent Protein (EGFP) gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter was injected into fertilized CD1 mouse eggs. Founder line 1 was established on a C57BL/6 background prior to mating to NOD. In 2008, the Type 1 Diabetes Resource received this transgenic NOD strain at generation N14 and mated to NOD/ShiLtJ (Stock No. 001976) for one generation prior to sibling mating.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. |
Allele Name | transgene insertion 1, Manami Hara |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | MIP:GFP; MIP-EGFP; MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); Tg(Ins1-GFP)1Hara |
Gene Symbol and Name | Tg(Ins1-EGFP)1Hara, transgene insertion 1, Manami Hara |
Gene Synonym(s) | |
Promoter | Ins1, insulin I, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter (a 8.5 kb region including -8.5 to +12 bp relative to the transcriptional start site) was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. |
Mutations Made By | Manami Hara, University of Chicago |
When maintaining a live colony, hemizygous mice may be bred with wildtype (noncarrier) mice or NOD/ShiLtJ inbred mice (Stock No. 001976).
When using the NOD.B6-MIP-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #008173 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Ins1-EGFP)1Hara |
Frozen Mouse Embryo | NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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