These Snord116del mice may be useful in studying growth and feeding regulation, mechanisms of obesity, and pathophysiology of Prader-Willi syndrome. Of note, mice harboring a loxP-flanked Snord116 cluster are also available (see Stock No. 008118).
Dr. Uta Francke, Stanford University School of Medicine
Genetic Background | Generation |
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?+pN1
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Snord116 | small nucleolar RNA, C/D box 116 cluster |
Starting at:
$255.00 Domestic price for female 6-week |
333.51 Domestic price for breeder pair |
$2,854.50 Domestic price Cryo Recovery |
Mice homozygous for this Snord116del (1-loxP or knockout) allele are viable and fertile. As the Snord116 gene cluster is imprinted and expressed only from the paternal allele, mice with paternal inheritance of the deletion lack expression of the targeted Snord116 small nucleolar RNAs (snoRNAs) gene cluster in brain tissues. Similarly, paternal transmission of the mutant allele is required to obtain the mutant phenotype in offspring. Affected heterozygotes (paternal deleted/maternal wildtype) recapitulate a subset of Prader-Willi syndrome (PWS) characteristics, including early-onset postnatal growth retardation, delayed sexual maturation, increased anxiety, motor learning deficit and hyperphagia (but not obesity). Other reported abnormalities include altered metabolic fuel usage, prolonged meal time, and increased levels of circulating ghrelin. These Snord116del mice may be useful in studying growth and feeding regulation, mechanisms of obesity, and pathophysiology of Prader-Willi syndrome.
Of note, mice harboring a loxP-flanked Snord116 cluster are also available (see Stock No. 008118).
Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts. Chimeric males were bred to albino B6(Cg)-Tyrc-2J/J (Stock No. 000058) females. The mutant 1-loxP mice were then bred to C57BL/6J inbred mice for 5 generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1.1, Uta Francke |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | 1-lox; 1-loxp; Snord116del |
Gene Symbol and Name | Snord116, small nucleolar RNA, C/D box 116 cluster |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 7 |
Molecular Note | Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts. |
Mutations Made By | Dr. Uta Francke, Stanford University School of Medicine |
When maintaining a live colony, heterozygous males are bred with wildtype females (or C57BL/6J inbred females). As imprinting of the Snord116 gene cluster is determined via paternal inheritance, paternal transmission of the mutant allele is required to obtain the mutant phenotype.
When using the 1-loxp (KO) , Snord116del mouse strain in a publication, please cite the originating article(s) and include JAX stock #008149 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Snord116<tm1.1Uta> |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1.1Uta>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1.1Uta>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1.1Uta>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1.1Uta>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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