As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and pathophysiology of Prader-Willi syndrome. Of note, mice harboring a deletion of the Snord116 cluster are also available (see Stock No. 008149).
Dr. Uta Francke, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Snord116 | small nucleolar RNA, C/D box 116 cluster |
Mice homozygous for this 2-loxP (floxed) allele are viable and fertile, with loxP sites flanking the Snord116 small nucleolar RNAs (snoRNAs) gene cluster. When bred to mice that express Cre recombinase, the resulting offspring will have this gene cluster deleted in the cre-expressing tissue(s). Because the Snord116 gene cluster is imprinted and only expressed from the paternal allele, breeding 2-loxP males with cre-expressing females may be required to generate deleted offspring with the knockout phenotype. The donating investigator reports that the distance between the two loxP sites (~140 kb) may reduce the recombination efficiency in somatic cells. As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and pathophysiology of Prader-Willi syndrome.
Of note, mice harboring a deletion of the Snord116 cluster are also available (see Stock No. 008149).
For example, when crossed to a strain expressing Cre recombinase in the female germline (see Stock No. 003651), this mutant mouse strain may be useful in studies of Prader-Willi syndrome.
Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with an FLP expressing plasmid to remove the two selection cassettes. The resulting 2-loxP ES cells (with a single loxP site just upstream, and a single loxP site just downstream of the Snord116 cluster) were injected into recipient blastocysts. Chimeric males were bred to albino B6(Cg)-Tyrc-2J/J (Stock No. 000058) females. The resulting females were then bred to C57BL/6 males (expressing cre in the female, but not male, germline (Zp3-cre; Stock No. 003651)). Offspring carrying the 2-loxP mutation (and not the Zp3-cre transgene) were selected and backcrossed to C57BL/6J inbred mice for 2 generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Uta Francke |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | 2-lox; 2-loxp |
Gene Symbol and Name | Snord116, small nucleolar RNA, C/D box 116 cluster |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 7 |
Molecular Note | Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived embryonic stem ES cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with an FLP expressing plasmid to remove the two selection cassettes. The resulting 2-loxP ES cells (with a single loxP site just upstream, and a single loxP site just downstream of the Snord116 cluster. |
Mutations Made By | Dr. Uta Francke, Stanford University School of Medicine |
When maintaining a live colony, homozygous mice may be bred together. As imprinting of the endogenous gene is determined via paternal inheritance, paternal transmission of the mutant allele may be required.
When using the 2-loxp (floxed) mouse strain in a publication, please cite the originating article(s) and include JAX stock #008118 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Snord116<tm1Uta> |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1Uta>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1Uta>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1Uta>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Snord116<tm1Uta>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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