B6;129-Gatad2a^tm1Rnu/J

Stock number: 008105
Product name: mp66α KO
Research areas: Developmental Biology Research

Description

Homozygous mutant mice show developmental abnormalities and die prior to embryonic day 12.5.

Detailed description

Homozygous targeted mutant mice die between implantation and embryonic day 12.5. There is great variability in phenotype, with some pups showing severe malformations, while others develop almost normally but arrest in development prior to their death. Virtually every organ of mutant embryos could be affected, resulting in dramatically smaller embryos and advanced stages of necrosis. Expression of the targeted gene has been assayed by Northern blot analysis of embryonic stem (ES) cells carrying the mutation and aberrant RNA was found; this is a loss of function mutation. These animals are useful in studies of development.

Development

A targeting vector was designed to replace exons 2-4 with a loxP-flanked neomycin resistance (pgk-neo) cassette. This mutation introduced a stop codon before the coiled-coil region (CR1) region of the gene. The vector was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric pups were identified and crossed with C57BL/6 females. In order to remove the floxed pgk-neo cassette, heterozygous mutant females were crossed to a CMV-cre male on a mixed C57BL/6-129SV background. The line was then maintained in the heterozygous condition on a mixed background by the donating laboratory by breeding heterozygous males of confirmed fertility with C57BL/6 and 129S2/SvPasCrl females (alternate generations).

Allele

Symbol: Gatad2a^tm1Rnu
Name: targeted mutation 1, Roel Nusse
MGI accession ID: MGI:3762633
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gatad2a
Marker name: GATA zinc finger domain containing 2A
Chromosome: 8
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to replace exons 2-4 with a loxP-flanked neomycin resistance (pgk-neo) cassette. This mutation introduced a stop codon before the coiled-coil region (CR1) region of the gene. In order to remove the floxed pgk-neo cassette, heterozygous mutant females were crossed to a CMV-cre male on a mixed C57BL/6-129SV background.