These Prdm1 floxed conditional mutant mice may be useful in generating conditional mutations to study humoral immune response, plasma memory B-cells, and B-lymphocyte development and differentiation.
Kathryn Calame, Columbia University
Genetic Background | Generation |
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?+pN1
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Prdm1 | PR domain containing 1, with ZNF domain |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
$2,854.50 Domestic price Cryo Recovery |
These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s).
When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response.
When bred to mice carrying Tg(Itgax-cre)1-1Reiz (Stock No. 008068), Cre recombinase expression in dendritic cells results in altered adaptive immune response and lupus-like serology.
A targeting vector containing a loxP site flanked neomycin selection cassette and a herpes simplex virus thymidine kinase gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted downstream of exon 5. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exons 6-8 were injected in C57BL/6J blastocysts. Resulting chimeric animals were backcrossed to C57BL/6J for 10 generations before arriving at The Jackson Laboratory.
Allele Name | targeted mutation 1, Kathryn Calame |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Blimp-1flox; Prdm1CA; Prdm1flox |
Gene Symbol and Name | Prdm1, PR domain containing 1, with ZNF domain |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 10 |
Molecular Note | LoxP sites were inserted to flank exons 6-8, encompassing sequence that encodes the zinc finger motifs. |
Mutations Made By | Kathryn Calame, Columbia University |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Prdm1F mouse strain in a publication, please cite the originating article(s) and include JAX stock #008100 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous for Prdm1<tm1Clme> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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