B6.129-Prdm1^tm1Clme/J

Stock number: 008100
Product name: Prdm1^F
Research areas: Research Tools

Description

These Prdm1 floxed conditional mutant mice may be useful in generating conditional mutations to study humoral immune response, plasma memory B-cells, and B-lymphocyte development and differentiation.

Detailed description

These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response.

When bred to mice carrying Tg(Itgax-cre)1-1Reiz (Stock No. 008068), Cre recombinase expression in dendritic cells results in altered adaptive immune response and lupus-like serology.

Development

A targeting vector containing a loxP site flanked neomycin selection cassette and a herpes simplex virus thymidine kinase gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted downstream of exon 5. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exons 6-8 were injected in C57BL/6J blastocysts. Resulting chimeric animals were backcrossed to C57BL/6J for 10 generations before arriving at The Jackson Laboratory.

Allele

Symbol: Prdm1^tm1Clme
Name: targeted mutation 1, Kathryn Calame
MGI accession ID: MGI:3605621
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Prdm1
Marker name: PR domain containing 1, with ZNF domain
Chromosome: 10
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: LoxP sites were inserted to flank exons 6-8, encompassing sequence that encodes the zinc finger motifs.