These D4R-mutant mice harbor a dopamine receptor 4 (Drd4; also called dopamine D4 receptor (D4R)) targeted mutation where exon 2 is replaced with a neomycin resistance cassette, and may be useful in studying dopamine/neurotransmitter function, drug addiction, Parkinson's disease, and schizophrenia or other psychoses.
Dr. Malcolm J Low, University of Michigan Medical School
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Drd4 | dopamine receptor D4 |
Mice homozygous for this targeted mutation (D4R-/-) are viable and fertile. With exon 2 of the mutant locus deleted, the truncated transcript is predicted to produce a 131 amino acid mutant polypeptide. Homozygous mice may exhibit locomotor supersensitivity to ethanol, cocaine, and methamphetamine, as well as alterations in dopamine synthesis and function, glutamate levels and metabolism, behavioral responses to novelty, and spontaneous locomotor activity in both novel and familiar environments. These D4R-mutant mice may be useful in studying dopamine/neurotransmitter function, drug addiction, Parkinson's disease, and schizophrenia or other psychoses.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to replace exon 2 of the targeted gene with a neomycin resistance cassette. The construct was electroporated into 129P2/OlaHsd-derived E14TG2A embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred to C57BL/6J females. Heterozygous D4R-mutant mice were bred to wildtype siblings or C57BL/6J mice for approximately 30 generations (including to C57BL/6J for 5 most recent generations) prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.
Allele Name | targeted mutation 1, David K Grandy |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | D4-; D4KO; D4R -; Drd4- |
Gene Symbol and Name | Drd4, dopamine receptor D4 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | A neomycin resistance cassette replaced exon 2. RT-PCR analysis demonstrated that a mutated transcript was produced from this allele that spliced exon 1 to exon 3. This mutation causes a shift in the reading frame that is predicted to result in the production of a truncated protein. |
Mutations Made By | David Grandy, Oregon Heath Sciences University |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129P2-Drd4tm1Dkg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008084 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Drd4<tm1Dkg> |
Frozen Mouse Embryo | B6.129P2-Drd4<tm1Dkg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Drd4<tm1Dkg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Drd4<tm1Dkg>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Drd4<tm1Dkg>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.