JAX Mouse Strain Datasheet - 008077

129S1/Sv-Bche^tm1Loc/J

Stock No:: 008077

Cryo Recovery

Overview

Also Known As: 129S1-BChE

These BChE (butyrylcholinesterase) mutant mice are a model for human butyrylcholinesterase deficiency and may be useful for studying metabolic effects of organophosphorus toxicants or nerve agents, neurotransmitter function, and anti-Alzheimer's drug therapies.

Donating Investigator

Dr. Oksana Lockridge, Eppley Inst. (Univ of Nebraska Med Cntr)

Genetic overview

Genetic Background	Generation

Bche^tm1Loc

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Bche	butyrylcholinesterase

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Research Applications

Cancer Research
Metabolism Research
Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for this BChE mutant allele are viable and fertile with no reported spontaneous abnormalities. All tissues and plasma from homozygous mice are devoid of BChE activity. As BChE (butyrylcholinesterase) is a bioscavenger molecule protecting acetylcholinesterase (AChE) activity against nerve agents and organophosphates, homozygous BChE-deficiency leads to impaired protection from toxic compounds. These BChE mutant mice are a model for human butyrylcholinesterase deficiency and may be useful for studying metabolic effects of organophosphorus toxicants or nerve agents, neurotransmitter function, and anti-Alzheimer's drug therapies.

Of note, the donating investigator has multiple strains available that may be useful for testing toxic compounds, including the AChE-deficient (Stock No. 005987), G117H BChE transgenic (Stock No. 007577), and BChE-deficient (see Stock No. 008077 and Stock No. 008087) mice.

Development

The targeting vector was designed to replace the sequence containing the splice junction between intron 1 and exon 2 through the entire signal peptide (including the translation start site and the first 102 amino acids of the encoded BChE protein) with a neomycin cassette. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ inbred mice. Mutant mice were backcrossed to 129S1/SvImJ for more than 10 generations prior to arrival at The Jackson Laboratory.

Control Suggestions

002448 129S1/SvImJ

Additional Information

Considerations for Choosing Controls

Selected References

Duysen EG; Li B; Darvesh S; Lockridge O. 2007. Sensitivity of butyrylcholinesterase knockout mice to (--)-huperzine A and donepezil suggests humans with butyrylcholinesterase deficiency may not tolerate these Alzheimer's disease drugs and indicates butyrylcholinesterase function in neurotransmission. Toxicology 233(1-3):60-9. PubMed: 17194517 MGI: J:130048
Li B; Duysen EG; Saunders TL; Lockridge O. 2006. Production of the butyrylcholinesterase knockout mouse. J Mol Neurosci 30(1-2):193-5. PubMed: 17192674 MGI: J:124975

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Genetics

Bche^tm1Loc

Allele Symbol: Bche^tm1Loc

Allele Name	targeted mutation 1, Oksana Lockridge
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	BChE KO; BChE^-
Gene Symbol and Name	Bche, butyrylcholinesterase
Gene Synonym(s)	C730038G20Rik; C730038G20Rik; CHE1; CHE2; E1; RIKEN cDNA C730038G20 gene
Strain of Origin	129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Chromosome	3
Molecular Note	891bp of the gene is replaced with a neomycin selection cassette, including 485bp from the 3' end of intron 1 and 406bp from the 5' end of exon 2. The region encoding the entire signal peptide, the translation start site, and the first 102 amino acids of the mature protein is deleted.
Mutations Made By	Dr. Oksana Lockridge, Eppley Inst. (Univ of Nebraska Med Cntr)

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cancer Research
- Toxicology
Metabolism Research
Neurobiology Research
- Neuromuscular defects
- Neurotransmitter Receptor and Synaptic Vesicle Defects
- Receptor Defects
  - cholinergic receptor
- Tremor Defects
Research Tools
- Cancer Research
- Metabolism Research
- Neurobiology Research
- Sensorineural Research
- Toxicology Research
  - drug metabolism
  - drug/compound testing

Genotype: Bche^tm1Loc related

Neurobiology Research
- Alzheimer's Disease

Mammalian Phenotype Terms by Genotype

Genotype: Bche^tm1Loc/Bche⁺
involves: 129S1/Sv

mortality/aging

increased sensitivity to xenobiotic induced morbidity/mortality
- (-)-huperzin A-treated mice die within 24 hours unlike similarly treated controls
- donepezil-treated mice die within 24 hours unlike similarly treated controls

homeostasis/metabolism phenotype

increased physiological sensitivity to xenobiotic
- (-)-huperzin A-treated mice exhibit protosis, salivation, and lacrimation with death occurring within 24 hours of treatment unlike similarly treated mice
- donepezil-treated mice fail to recover normal body temperature unlike similarly treated wild-type mice
- treatment with donepezil induces intermediate symptoms with all mice dying or moribund within 24 hours unlike similarly treated wild-type mice that recover within 24 hours

increased sensitivity to xenobiotic induced morbidity/mortality
- (-)-huperzin A-treated mice die within 24 hours unlike similarly treated controls
- donepezil-treated mice die within 24 hours unlike similarly treated controls

Genotype: Bche^tm1Loc/Bche^tm1Loc
involves: 129S1/Sv

mortality/aging

increased sensitivity to xenobiotic induced morbidity/mortality
- (-)-huperzin A-treated mice die within 10 minutes unlike similarly treated controls
- donepezil-treated mice die within 3 hours unlike similarly treated controls

reproductive system phenotype

reproductive system phenotype
- mice are fertile

homeostasis/metabolism phenotype

increased physiological sensitivity to xenobiotic
- (-)-huperzin A-treated mice exhibit protosis, salivation, and lacrimation with death occurring within 10 minutes of treatment unlike similarly treated mice
- however, mice exhibit a normal reaction to CPO and echothiophate
- treatment with donepezil induces clonic, jerking convulsions and death within 3 hours unlike similarly treated wild-type mice that recover within 24 hours

increased sensitivity to xenobiotic induced morbidity/mortality
- (-)-huperzin A-treated mice die within 10 minutes unlike similarly treated controls
- donepezil-treated mice die within 3 hours unlike similarly treated controls

References

Duysen EG; Li B; Darvesh S; Lockridge O. 2007. Sensitivity of butyrylcholinesterase knockout mice to (--)-huperzine A and donepezil suggests humans with butyrylcholinesterase deficiency may not tolerate these Alzheimer's disease drugs and indicates butyrylcholinesterase function in neurotransmission. Toxicology 233(1-3):60-9. PubMed: 17194517 MGI: J:130048
Li B; Duysen EG; Saunders TL; Lockridge O. 2006. Production of the butyrylcholinesterase knockout mouse. J Mol Neurosci 30(1-2):193-5. PubMed: 17192674 MGI: J:124975

Additional - Bche^tm1Loc related

Chen VP; Gao Y; Geng L; Parks RJ; Pang YP; Brimijoin S. 2015. Plasma butyrylcholinesterase regulates ghrelin to control aggression. Proc Natl Acad Sci U S A 112(7):2251-6. PubMed: 25646463 MGI: J:220003
Chen VP; Gao Y; Geng L; Stout MB; Jensen MD; Brimijoin S. 2016. Butyrylcholinesterase Deficiency Promotes Adipose Tissue Growth and Hepatic Lipid Accumulation in Male Mice on High-Fat Diet. Endocrinology 157(8):3086-95. PubMed: 27300766 MGI: J:239969
Duysen EG; Li B; Lockridge O. 2009. The butyrylcholinesterase knockout mouse a research tool in the study of drug sensitivity, bio-distribution, obesity and Alzheimer's disease. Expert Opin Drug Metab Toxicol 5(5):523-8. PubMed: 19416087 MGI: J:148458
Duysen EG; Lockridge O. 2011. Induction of plasma acetylcholinesterase activity in mice challenged with organophosphorus poisons. Toxicol Appl Pharmacol 255(2):214-20. PubMed: 21767560 MGI: J:174614
Haupt M; Kauschke V; Sender J; Kampschulte M; Kovtun A; Durselen L; Heiss C; Lips KS. 2015. Bone status of adult female butyrylcholinesterase gene-deficient mice. Int Immunopharmacol 29(1):208-14. PubMed: 26138460 MGI: J:226933
Maurice T; Strehaiano M; Simeon N; Bertrand C; Chatonnet A. 2016. Learning performances and vulnerability to amyloid toxicity in the butyrylcholinesterase knockout mouse. Behav Brain Res 296:351-60. PubMed: 26306824 MGI: J:227529
Reid GA; Darvesh S. 2015. Butyrylcholinesterase-knockout reduces brain deposition of fibrillar beta-amyloid in an Alzheimer mouse model. Neuroscience 298:424-35. PubMed: 25931333 MGI: J:224492

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: tjlca@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

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Animal Care and Use for SERVICES

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: 129S1-BChE

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Bchetm1Loc

Allele Symbol: Bchetm1Loc

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Bchetm1Loc related

Mammalian Phenotype Terms by Genotype

Genotype: Bchetm1Loc/Bche+involves: 129S1/Sv

mortality/aging

homeostasis/metabolism phenotype

Genotype: Bchetm1Loc/Bchetm1Locinvolves: 129S1/Sv

mortality/aging

reproductive system phenotype

homeostasis/metabolism phenotype

References

Additional - Bchetm1Loc related

Genotyping Protocols

Breeding Considerations

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Bche^tm1Loc

Allele Symbol: Bche^tm1Loc

Genotype: Bche^tm1Loc related

Genotype: Bche^tm1Loc/Bche⁺
involves: 129S1/Sv

Genotype: Bche^tm1Loc/Bche^tm1Loc
involves: 129S1/Sv

Additional - Bche^tm1Loc related