These floxed mutant mice possess loxP sites flanking exon 6 of the Cd44 gene. This strain may be useful for generating conditional mutations in studies of multiple sclerosis (MS) and inflammatory diseases.
Ursula Gunthert, University of Basel
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cd44 | CD44 antigen |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice possess loxP sites on either side of exon 6 of the targeted gene. RT-PCR analysis reveals that exon 7 is not expressed in the floxed mice, and Southern blots confirm that 1.6kb of exon 7 was deleted during homologous recombination. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 6 and exon 7 deleted in the cre-expressing tissue(s). According to the donating investigator, homozygotes exhibit reduced incidence and severity of induced experimental autoimmune encephalomyelitis (EAE) when compared to wildtype controls. This mutant mouse strain may be useful in studies of multiple sclerosis (MS) and inflammatory diseases.
NOTE: Stock No. 008008 mice are on a unique "SJL/R" genetic background; the "SJL/R" strain was maintained by the Erasmus Medical Center (Rotterdam, The Netherlands) as a unique substrain of SJL that showed more favorable responses to EAE induction.
NOTE: On a 129/Sv genetic background, homozygotes are resistant to trinitrobenzene sulfonic acid (TNBS)-induced colitis.
A targeting vector containing a loxP site-flanked neomycin resistance cassette inserted upstream and a third loxP site inserted downstream of exon 6 of the targeted gene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Approximately 1.6kb of exon 7 also was deleted from the targeted gene during homologous recombination. The resulting chimeric mice were bred to "SJL/R" mice (from Erasmus Medical Center, Rotterdam, The Netherlands) for 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, female and male mutant mice were bred together to preserve the unique genetic background of this strain.
NOTE: The "SJL/R" strain was maintained by the Erasmus Medical Center (Rotterdam, The Netherlands) as a unique substrain of SJL that showed more favorable responses to EAE induction. A 27 SNP (single nucleotide polymorphism) panel analysis performed on the Cd44-mutant mice that were rederived by The Jackson Laboratory revealed 4 loci that were homozygous for markers other than SJL/J. These loci are on chromosome 3 (approximately 16.3 cM), chromosome 9 (approximately 6.6 cM), chromosome 11 (approximately 9.8 cM), and chromosome 18 (approximately 26.2 cM). The Jackson Laboratory obtained "SJL/R" splenocyte DNA from the Erasmus Medical Center and report an identical SNP profile using the same 27 SNP panel analysis.
NOTE: Stock No. 008008 mice are on a unique "SJL/R" genetic background; the "SJL/R" strain was maintained by the Erasmus Medical Center (Rotterdam, The Netherlands) as a unique substrain of SJL that showed more favorable responses to EAE induction. )
Allele Name | targeted mutation 1, Ursula Gunthert |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD44v7- |
Gene Symbol and Name | Cd44, CD44 antigen |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
Molecular Note | A loxP site was inserted 3' of exon v6 while a neomycin resistance cassette with a 5' loxP site was inserted 5' of exon v6. During insertion, 1.6 kb of sequence was lost in the exon v7 region leading to deficient expression of this variant in homozygous mutant mice. |
Mutations Made By | Ursula Gunthert, University of Basel |
When maintaining a live colony, these mice can be bred as homozygotes.
NOTE: Stock No. 008008 mice are on a unique "SJL/R" genetic background; the "SJL/R" strain was maintained by the Erasmus Medical Center (Rotterdam, The Netherlands) as a unique substrain of SJL that showed more favorable responses to EAE induction.
When using the CD44v7 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #008008 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Cd44<tm1Ugu> |
Frozen Mouse Embryo | SJL.129-Cd44<tm1Ugu>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | SJL.129-Cd44<tm1Ugu>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | SJL.129-Cd44<tm1Ugu>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | SJL.129-Cd44<tm1Ugu>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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