B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J

Stock number: 008004
Product name: BRO; Syb 2-ECFP transgenic, UTSD#1896
Research areas: Neurobiology Research, Research Tools

Description

The mouse Thy1 gene promoter drives expression of a fusion protein made from ECFP and the bovine Vamp2 (Syb2) gene. Expression of the bovine gene is not eliminated. ECFP expression is neuron specific and signal may be found in various brain regions and neuromuscular junctions (NMJ).

Detailed description

Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical abnormalities. The mouse Thy1 gene promoter drives expression of a fusion protein made from ECFP and the bovine Vamp2 (Syb2) gene. Expression of the bovine gene is not eliminated. ECFP expression is neuron specific and signal may be found in various brain regions and neuromuscular junctions (NMJ).

Development

A transgenic vector incorporating an ECFP/bovine Vamp2 (Syb2) fusion protein directed by a mouse Thy1 promoter was created. Approximately 10 copies of the transgene were introduced to a mixed B6SJL (C57BL/6 x SJL) background.

Allele

Symbol: Tg(Thy1-ECFP/VAMP2)1Sud
Name: transgene insertion 1, Thomas C Sudhof
MGI accession ID: MGI:3773655
Generation method: Transgenic
Attributes: Reporter
Synonyms: BRO Syb2-ECFP
Marker symbol: Tg(Thy1-ECFP/VAMP2)1Sud
Marker name: transgene insertion 1, Thomas C Sudhof
Marker synonyms: BRO Syb2-ECFP
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: CFP,Cyan Fluorescent Protein,jellyfish
Site of expression: ECFP expression is neuron specific and signal may be found in various brain regions and neuromuscular junctions.
Molecular note: A transgenic vector incorporating an ECFP/bovine Vamp2 (Syb2) fusion protein directed by a mouse Thy1 promoter was created. Approximately 10 copies of the transgene were introduced to a mixed C57BL/6 x SJL background. ECFP expression is neuron specific and signal may be found in various brain regions and neuromuscular junctions (NMJ).