These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. This mutant mouse strain may be useful in studies of mitochondrial transport.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations including retinal cells and in all motor axons. Coronal brain sections reveal a fluorescence pattern showing somatosensory cortex barrel morphology. Neuronal, mitochondrial and neuromuscular junction morphology appears normal in transgenic mice. Axonal mitochondrial density is similar to wildtype. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of mitochondrial transport.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBA mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published line C, was subsequently established. The mice were backcrossed for at least 5 generations to C57BL/6J.
Expressed Gene | COX8A, cytochrome c oxidase subunit 8A, human |
---|---|
Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
Site of Expression | mitochondria; many neuronal populations including retinal cells and all motor axons; coronal brain sections show pattern of somatosensory cortex barrel morphology |
Allele Name | transgene insertion C1, Jeff W Lichtman |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Thy1-CFP-MitoC |
Gene Symbol and Name | Tg(Thy1-CFP/COX8A)C1Lich, transgene insertion C1, Jeff W Lichtman |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | COX8A, cytochrome c oxidase subunit 8A, human |
Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
Site of Expression | mitochondria; many neuronal populations including retinal cells and all motor axons; coronal brain sections show pattern of somatosensory cortex barrel morphology |
Strain of Origin | Not Applicable |
Chromosome | UN |
General Note | Founder line C1 corresponds to the published line C. |
Molecular Note | A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized donor mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published line C, was subsequently established. |
Mutations Made By | Robert Burgess, The Jackson Laboratory |
When maintaining a live colony, these mice are bred as hemizygotes.
When using the B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007940 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Carrier or non-carrier for Tg(Thy1-CFP/COX8A)C1Lich |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.